Long-term moderate fertilization increases the complexity of soil microbial community and promotes regulation of phosphorus cycling genes to improve the availability of phosphorus in acid soil

Applied Soil Ecology, Год журнала: 2023, Номер 194, С. 105178 - 105178

Опубликована: Ноя. 7, 2023

Язык: Английский

---

Genome-Resolved Metagenomics and Denitrifying Strain Isolation Reveal New Insights into Microbial Denitrification in the Deep Vadose Zone

Environmental Science & Technology, Год журнала: 2024, Номер 58(5), С. 2323 - 2334

Опубликована: Янв. 24, 2024

The heavy use of nitrogen fertilizer in intensive agricultural areas often leads to nitrate accumulation subsurface soil and contamination groundwater, which poses a serious risk public health. Denitrifying microorganisms the subsoil convert gaseous forms nitrogen, thereby mitigating leaching into groundwater. Here, we investigated denitrifying deep vadose zone typical area China through microcosm enrichment, genome-resolved metagenomic analysis, bacteria isolation. A total 1000 metagenome-assembled genomes (MAGs) were reconstructed, resulting 98 high-quality, dereplicated MAGs that contained denitrification genes. Among them, 32 could not be taxonomically classified at genus or species level, indicating broader spectrum taxonomic groups is involved than previously recognized. denitrifier isolate library was constructed by using strategy combining high-throughput conventional cultivation techniques. Assessment characteristics both isolates demonstrated dominance truncated denitrification. Functional screening revealed highest activity two complete denitrifiers belonging Pseudomonas. These findings greatly expand current knowledge composition function subsoils. provided first pool subsoil-denitrifying facilitate development microbe-based technologies for attenuation

Язык: Английский

---

Comparison of DNA extraction methods on different sample matrices within the same terrestrial ecosystem

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Апрель 15, 2024

Abstract Metataxonomic studies of ecosystem microbiotas require the simultaneous processing samples with contrasting physical and biochemical traits. However, there are no published comparisons different DNA extraction kits to characterize main components terrestrial ecosystems. Here, our knowledge for first time, five were used investigate composition diversity microbiota a subset typically studied in ecosystems such as bulk soil, rhizosphere invertebrate taxa mammalian feces. kit was associated changes relative abundance hundreds ASVs, same samples, resulting significant differences alpha beta estimates their microbiotas. Importantly, impact on sample varies according type, feces soil showing most least consistent across kits, respectively. We show that MACHEREY–NAGEL NucleoSpin® Soil highest estimates, providing contribution overall diversity, indicated by computationally assembled reference communities, is recommended be any large-scale study

Язык: Английский

---

Fertilizing-induced changes in the nitrifying microbiota associated with soil nitrification and crop yield

The Science of The Total Environment, Год журнала: 2022, Номер 841, С. 156752 - 156752

Опубликована: Июнь 16, 2022

Язык: Английский

---

Metagenomic insights into genetic factors driving bacterial niche differentiation between bulk and rhizosphere soils

The Science of The Total Environment, Год журнала: 2023, Номер 891, С. 164221 - 164221

Опубликована: Май 31, 2023

Язык: Английский

---