bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Июль 9, 2024
Abstract
Climate
change
poses
significant
risks
to
freshwater
ecosystems,
potentially
exacerbating
harmful
cyanobacterial
blooms
and
antibiotic
resistance.
We
investigated
these
dual
threats
in
Cosseys
Reservoir,
New
Zealand,
by
simulating
short-term
warming
scenarios
assessing
the
role
of
oxidative
stress.
Microcosms
were
subjected
Base
(22°C),
Normal
(24°C),
projected
Future
(27°C)
temperatures,
with
additional
treatments
including
reactive
oxygen
species
(ROS).
Metagenomic
analysis
revealed
substantial
restructuring
microbial
communities
under
conditions.
Cyanobacterial
abundance
increased
from
6.11%
(initial)
20.53%
at
24°C
10.66%
27°C.
Notably,
ROS
addition
mimicked
effects
temperature
increase
on
proliferation.
Toxin-producing
families,
Microcystaceae
Nostocaceae
,
proliferated
significantly.
The
microcystin
synthesis
gene
(
mcy
)
showed
a
strong
positive
association
(R²
=
0.88)
abundance.
Moreover,
cyanobacteria
exhibited
enhanced
nutrient
acquisition
pstS
gene,
R²
0.69)
upregulated
nitrogen
metabolism
pathways
Concurrently,
we
observed
marked
resistance
(ARG)
rising
temperatures.
relative
multidrug
genes
was
consistently
high
across
all
conditions
(50.82
%
total
ARGs).
stress
further
intensified
ARG
proliferation,
particularly
for
efflux
pump
e.g.
acrB
adeJ
ceoB
emrB
MexK
muxB
).
Co-association
network
identified
key
antibiotic-resistant
bacteria
Streptococcus
pneumoniae
Acinetobacter
baylyi
ARGs
rpoB2
bacA
central
dissemination
This
study
demonstrates
that
even
modest
increases
(2-5°C)
can
promote
both
ecosystems
over
short
time
scales.
synergistic
underscore
complex
challenges
posed
climate
water
quality
public
health.
Highlights
Short-term
promotes
toxic
freshwater.
Harmful
their
metabolic
potential
Reactive
mimic
exacerbate
effects.
Oxidative
response
strongly
correlate
networks
identify
host
pathogen
spread.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Апрель 6, 2024
ABSTRACT
Conventional
activated
sludge
(CAS)
processes
represent
significant
reservoirs
of
antibacterial
resistance
genes
(ARGs).
However,
the
mechanisms
governing
ARG
proliferation
(enrichment
and
emergence)
in
CAS
remain
poorly
understood.
We
hypothesize
that
Reactive
Oxygen
Species
(ROS),
generated
by
oxygenation
aeration
tanks,
govern
proliferation.
Shotgun-metagenomics
assessment
abundance
across
treatment
stages
two
plants
handling
hospital
effluents
revealed
a
substantial
reduction
total
ARGs
treated
effluent.
specific
conferring
to
beta-lactams,
tetracyclines,
aminoglycosides,
rifamycins,
macrolides,
other
drug
classes
showed
marked
increase
CAS,
exhibiting
correlations
with
ROS
stress
response
genes.
Notably,
many
these
emerged
were
absent
upstream
processes.
Proof-of-concept
experiments
demonstrated
increased
levels
under
high
dissolved
oxygen
(8
mg/L)
compared
low
(2
conditions.
Both
ESKAPE
non-ESKAPE
host
pathogens
harbored
multiple
shared
ARGs,
indicating
horizontal
gene
transfer
facilitated
ROS-induced
responses,
including
antioxidant
defense
activation,
cell
damage,
DNA
mutation,
metabolic
adaptation,
efflux
pump
enhanced
transformation,
could
contribute
enrichment/emergence.
This
study
highlights
inadvertently
promotes
proliferation,
underscoring
need
manage
control
environmental
dissemination.
Abstract
Figure
ACS Environmental Au,
Год журнала:
2022,
Номер
3(2), С. 105 - 120
Опубликована: Дек. 22, 2022
Spread
of
antibiotic
resistance
genes
(ARGs)
by
conjugation
poses
great
challenges
to
public
health.
Application
pyroligneous
acids
(PA)
as
soil
amendments
has
been
evidenced
a
practical
strategy
remediate
pollution
ARGs
in
soils.
However,
little
is
known
about
PA
effects
on
horizontal
gene
transfer
(HGT)
conjugation.
This
study
investigated
the
woody
waste-derived
prepared
at
450°C
and
its
three
distillation
components
(F1,
F2,
F3)
different
temperatures
(98,
130,
220°C)
conjugative
plasmid
RP4
within
Environmental Science & Technology,
Год журнала:
2022,
Номер
56(21), С. 15130 - 15140
Опубликована: Авг. 19, 2022
Anaerobic
digestion
(AD)
has
been
widely
employed
for
converting
various
biowastes
into
renewable
energy.
However,
AD
of
gentamicin
mycelial
residues
(GMRs,
a
byproduct
production)
is
limited
by
ammonia
inhibition
and
antimicrobial
resistance
risk.
Compared
to
mesophilic
(MMAD)
GMRs,
this
study
looked
three
semicontinuous
processes,
i.e.,
codigestion
with
wheat
straw,
thermophilic
(TAcoD),
at
shortened
retention
time
(RT).
Results
showed
that
stable
safe
could
be
achieved
under
suitable
operating
conditions.
Co-digestion
effectively
mitigate
the
adverse
effect
inhibition.
The
methane
production
increased
35.86%
in
TAcoD
compared
MMAD
43.99%
hazardous
waste
was
reduced
TAcoD.
Concerning
system,
degraded
efficiently
degradation
process
not
involved
expression
antibiotic
genes
(ARGs)
related
modifying
enzyme.
Effective
removal
ARGs
strategies
associated
higher
reduction
bacterial
abundance
potential
hosts.
In
addition,
changes
relevant
proteins
transformation
conjugation
as
predicted
PICRUSt
suggested
condition
shorter
RT
were
conducive
dissemination
risks
ARGs.
Foods,
Год журнала:
2024,
Номер
13(20), С. 3257 - 3257
Опубликована: Окт. 13, 2024
Antibiotic-resistant
genes
(ARGs)
pose
a
significant
threat
to
the
global
food
transformation
system.
The
increasing
prevalence
of
ARGs
in
has
elicited
apprehension
about
public
health
safety.
widespread
dissemination
products,
driven
by
inappropriate
use
antibiotics,
presents
adversity
for
safety
emerging
future
sources
such
as
edible
insects.
As
world
faces
challenges
related
security,
climate
change,
and
antibiotic
resistance,
insects
offer
sustainable
resilient
source.
intriguing
possibility
serving
less
conducive
environment
compared
livestock
warrants
further
exploration
investigation.
In
this
recent
work,
we
listed
from
detected
so
far
vitro
approaches
aimed
construct
fair
comparison
with
based
on
relevant
genes.
We
also
presented
our
argument
analyzing
factors
that
might
be
responsible
ARG
abundance
vs.
Livestock
have
diverse
gut
microbiota,
their
diets
differ
antibiotics.
Consequently,
may
vary
well.
addition,
processed
lower
levels
than
raw
ones.
hypothesize
could
potentially
contain
exhibit
diminished
ability
disseminate
relative
livestock.
A
regulatory
framework
help
intercept
ARGs.
Due
diligence
should
taken
when
marketing
consumption.
