Estimating the minimum number of SARS-CoV-2 infected cases needed to detect viral RNA in wastewater: To what extent of the outbreak can surveillance of wastewater tell us?

Environmental Research, Год журнала: 2021, Номер 195, С. 110748 - 110748

Опубликована: Янв. 17, 2021

There is increasing interest in wastewater-based epidemiology (WBE) of SARS-CoV-2 RNA to serve as an early warning system for a community. Despite successful detection wastewaters sampled from multiple locations, there still no clear idea on the minimal number cases community that are associated with positive virus wastewater. To address this knowledge gap, we septic tank (n = 57) and biological activated sludge 52) located on-site hospital. The hospital providing treatment infected patients, hospitalized patients per day known. It was observed depending which nucleocapsid gene targeted by means RT-qPCR, range 253-409 out 10,000 persons required prior detecting weak correlation between N1 N2 abundances wastewater cases. This however not N3 gene. frequency also higher than Furthermore, genes were detected at lower partially treated tank. In particular, abundance water quality parameters such total organic carbon pH. instances detection, average reduced 50 70% levels tank, suggesting degradation fragments already occurring stages process.

Язык: Английский

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Direct RT-qPCR assay for SARS-CoV-2 variants of concern (Alpha, B.1.1.7 and Beta, B.1.351) detection and quantification in wastewater

Environmental Research, Год журнала: 2021, Номер 201, С. 111653 - 111653

Опубликована: Июль 7, 2021

Язык: Английский

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A review on presence, survival, disinfection/removal methods of coronavirus in wastewater and progress of wastewater-based epidemiology

Journal of environmental chemical engineering, Год журнала: 2020, Номер 8(5), С. 104317 - 104317

Опубликована: Авг. 5, 2020

Язык: Английский

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Methods Evaluation for Rapid Concentration and Quantification of SARS-CoV-2 in Raw Wastewater Using Droplet Digital and Quantitative RT-PCR

Food and Environmental Virology, Год журнала: 2021, Номер 13(3), С. 303 - 315

Опубликована: Июль 22, 2021

Wastewater surveillance of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an emerging public health tool to understand the spread Coronavirus Disease 2019 (COVID-19) in communities. The performance different virus concentration methods and PCR needs be evaluated ascertain their suitability for use detection SARS-CoV-2 wastewater. We ultrafiltration polyethylene glycol (PEG) precipitation concentrate from sewage wastewater treatment plants upstream network (e.g., manholes, lift stations). Recovery viruses by was determined using Phi6 bacteriophage as a surrogate enveloped viruses. Additionally, presence all samples reverse transcription quantitative (RT-qPCR) droplet digital (RT-ddPCR), targeting three genetic markers (N1, N2 E). Using spiked samples, recoveries were estimated at 2.6-11.6% ultrafiltration-based 22.2-51.5% PEG precipitation. There no significant difference recovery efficiencies (p < 0.05) between procedure with without 16 h overnight incubation, demonstrating feasibility obtaining same day results. more often detected RT-ddPCR than RT-qPCR higher sensitivity precision. While RT-ddPCR, levels E gene almost below limit RT-qPCR. Collectively, our study suggested effective low-cost which allows large number processed simultaneously routine monitoring SARS-CoV-2. can implemented absolute quantification matrices.

Язык: Английский

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Making waves: Wastewater surveillance of SARS-CoV-2 in an endemic future

Water Research, Год журнала: 2022, Номер 219, С. 118535 - 118535

Опубликована: Май 3, 2022

Язык: Английский

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