The Pseudomonas ligninolytic catalytic network reveals the importance of auxiliary enzymes in lignin biocatalysts

Proceedings of the National Academy of Sciences, Год журнала: 2025, Номер 122(4)

Опубликована: Янв. 24, 2025

Lignin degradation by biocatalysts is a key strategy to develop plant-based sustainable carbon economy and thus alleviate global climate change. This process involves synergy between ligninases auxiliary enzymes. However, enzymes within secretomes, which are composed of thousands enzymes, remain enigmatic, although several ligninolytic have been well characterized. Moreover, it challenge understand synergistic lignin via diverse array especially in bacterial systems. In this study, the coexpression network periplasmic proteome uncovers potential accessory for B-type dye-decolorizing peroxidases (DypBs) Pseudomonas putida A514. The catalytic DypBs-based multienzyme complex DypBs couple with quinone reductases nitroreductase participate redox cycling. They work superoxide dismutase induce Fenton reaction oxidation. A synthetic enzyme cocktail (SEC), recruiting 15 was consequently designed four functions. It overcomes limitation repolymerization, exhibiting capacity comparable that native secretome. Importantly, we reveal mechanism SEC-A514 cell system, incorporates advantages vitro catalysis vivo microbial catabolism. Chemical analysis shows system significantly reduces molecular weight lignin, substantially extends spectra functional groups, efficiently metabolizes derivatives. As result, 25% utilized, its average reduced 27%. Our study advances knowledge lignin-degrading multienzymes provides viable strategy.

Язык: Английский

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Insights on microbial enzymes mediated biodegradation of azo dyes: a sustainable strategy for environment clean up

Bioremediation Journal, Год журнала: 2025, Номер unknown, С. 1 - 43

Опубликована: Май 12, 2025

Язык: Английский

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A critical review on biomass-based sustainable biorefineries using nanobiocatalysts: Opportunities, challenges, and future perspectives

Bioresource Technology, Год журнала: 2022, Номер 363, С. 127926 - 127926

Опубликована: Сен. 12, 2022

Язык: Английский

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Advances in graphene oxide based nanobiocatalytic technology for wastewater treatment

Environmental Nanotechnology Monitoring & Management, Год журнала: 2022, Номер 17, С. 100647 - 100647

Опубликована: Янв. 20, 2022

Язык: Английский

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Microbial degradation and transformation of benzo[a]pyrene by using a white-rot fungus Pleurotus eryngii F032

Chemosphere, Год журнала: 2022, Номер 307, С. 136014 - 136014

Опубликована: Авг. 12, 2022

Язык: Английский

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