Journal of environmental chemical engineering, Год журнала: 2024, Номер 12(3), С. 112953 - 112953
Опубликована: Май 6, 2024
Язык: Английский
Journal of Hazardous Materials, Год журнала: 2022, Номер 441, С. 129848 - 129848
Опубликована: Авг. 28, 2022
Язык: Английский
Процитировано
90
Water Research, Год журнала: 2022, Номер 218, С. 118451 - 118451
Опубликована: Апрель 13, 2022
Язык: Английский
Процитировано
78
The Science of The Total Environment, Год журнала: 2022, Номер 864, С. 161023 - 161023
Опубликована: Дек. 18, 2022
The early warning and tracking of COVID-19 prevalence in the community provided by wastewater surveillance has highlighted its potential for much broader viral disease surveillance. In this proof-of-concept study, 46 samples from four treatment plants (WWTPs) Queensland, Australia, were analyzed presence abundance 13 respiratory viruses, results compared with reported clinical cases. viruses concentrated using adsorption-extraction (AE) method, extracted nucleic acids qPCR RT-qPCR. Among tested, bocavirus (BoV), parechovirus (PeV), rhinovirus A (RhV A) B B) detected all samples. All tested except influenza virus (IBV) sample at least one WWTP. BoV was greatest concentration (4.96-7.22 log
Язык: Английский
Процитировано
75
Water Research, Год журнала: 2022, Номер 213, С. 118132 - 118132
Опубликована: Фев. 3, 2022
Effective wastewater surveillance of SARS-CoV-2 RNA requires the rigorous characterization limit detection resulting from entire sampling process - (PLOD). Yet to date, no studies have gone beyond quantifying assay (ALOD) for RT-qPCR or RT-dPCR assays. While ALOD is lowest number gene copies (GC) associated with a 95% probability in single PCR reaction, PLOD represents sensitivity method after considering efficiency all processing steps (e.g., sample handling, concentration, nucleic acid extraction, and assays) determine GC matrix specific detection. The primary objective this study was estimate combination concentration extraction six assays: five assays (US CDC N1 N2, China N ORF1ab (CCDC CCDC ORF1ab), E_Sarbeco RT-qPCR, one RT-dPCR) using two models (exponential survival cumulative Gaussian). An adsorption (AE) (i.e., virus on membrane membrane) used concentrate gamma-irradiated seeded into 36 samples. Overall, US had ALODs (< 10 GC/reaction) PLODs (<3,954 GC/50 mL; detection) regardless seeding level model used. Nevertheless, consistent amplification rates decreased when levels were < 2.32 × 103 mL even Consequently, concentrations are expected be low, it may necessary improve positive by analyzing additional field RT-PCR replicates. To best our knowledge, first assess provides important insights analytical limitations trace wastewater.
Язык: Английский
Процитировано
65
PLoS ONE, Год журнала: 2023, Номер 18(4), С. e0284370 - e0284370
Опубликована: Апрель 12, 2023
Wastewater-based epidemiology (WBE) has become a valuable tool for monitoring SARS-CoV-2 infection trends throughout the COVID-19 pandemic. Population biomarkers that measure relative human fecal contribution to normalize wastewater concentrations are needed improved analysis and interpretation of community trends. The Centers Disease Control Prevention National Wastewater Surveillance System (CDC NWSS) recommends using flow rate or indicators as population normalization factors. However, there is no consensus on which factor performs best. In this study, we provided first multistate assessment effects (crAssphage, F+ Coliphage, PMMoV) correlation cases CDC NWSS dataset 182 communities across six U.S. states. Flow normalized produced strongest with cases. from three were significantly lower than rate. Additionally, reverse transcription droplet digital polymerase chain reaction (RT-ddPCR) values over samples analyzed real-time quantitative (rRT-qPCR). Our shows utilizing RT-ddPCR generate between
Язык: Английский
Процитировано
29
iScience, Год журнала: 2023, Номер 26(7), С. 107019 - 107019
Опубликована: Июнь 9, 2023
Язык: Английский
Процитировано
27
The Science of The Total Environment, Год журнала: 2024, Номер 935, С. 173223 - 173223
Опубликована: Май 16, 2024
Язык: Английский
Процитировано
12
ACS ES&T Water, Год журнала: 2022, Номер 2(12), С. 2460 - 2470
Опубликована: Окт. 10, 2022
In the context of COVID-19 pandemic, wastewater-based epidemiology (WBE) emerged as a useful tool to account for prevalence SARS-CoV-2 infections on population scale. this study, we analyzed wastewater samples from three large (>300,000 people served) and four small (<25,000 communities throughout southern Germany August December 2021, capturing fourth infection wave in dominated by Delta variant (B.1.617.2). As dilution can skew biomarker concentrations wastewater, normalization parameters improve relationship between data clinical data. investigated suitability performance various parameters. Influent flow showed strong relationships precipitation data; accordingly, flow-normalization reacted distinctly events. Normalization surrogate viruses CrAssphage pepper mild mottle virus varying different sampling sites. The best was achieved with mixed fecal indicator calculated both viruses. Analyzing temporal spatial variation proved be explain performance. Overall, our findings indicate that viruses, flow, hydro-chemical is site-specific. We recommend testing individually specific sewage systems.
Язык: Английский
Процитировано
28
Epidemics, Год журнала: 2024, Номер 49, С. 100793 - 100793
Опубликована: Сен. 26, 2024
Язык: Английский
Процитировано
6
The Science of The Total Environment, Год журнала: 2022, Номер 852, С. 158448 - 158448
Опубликована: Сен. 2, 2022
Язык: Английский
Процитировано
21