Honey bee nutritional ecology: From physiology to landscapes

Advances in insect physiology, Год журнала: 2023, Номер unknown, С. 289 - 345

Опубликована: Янв. 1, 2023

Язык: Английский

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The neonicotinoid acetamiprid reduces larval and adult survival in honeybees (Apis mellifera) and interacts with a fungicide mixture

Environmental Pollution, Год журнала: 2024, Номер 360, С. 124643 - 124643

Опубликована: Авг. 2, 2024

Plant protection products (PPPs), which are frequently used in agriculture, can be major stressors for honeybees. They have been found abundantly the beehive, particularly pollen. Few studies analysed effects on honeybee larvae, and little is known about of insecticide-fungicide-mixtures, although this a highly realistic exposure scenario. We asked whether combination insecticide fungicides would affect developing bees. Honeybee larvae (Apis mellifera carnica) were reared vitro larval diets containing different PPPs at two concentrations, derived from residues neonicotinoid acetamiprid, combined boscalid/dimoxystrobin mixture all three substances. Mortality was assessed larval, pupal, adult stages, size weight newly emerged bees measured. The treatment higher concentrations significantly reduced survival. Interestingly, survival not affected by high concentrated insecticide-fungicides-mixture. However, negative synergistic caused low insecticide-fungicides-mixture, had no effect when applied alone. lower led to lighter bees, unaffected. Our results suggest that environmental relevant harmful To fully understand interaction PPPs, more combinations should studied social solitary with possibly sensitivities.

Язык: Английский

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Biochemical responses, feeding and survival in the solitary bee Osmia bicornis following exposure to an insecticide and a fungicide alone and in combination

Environmental Science and Pollution Research, Год журнала: 2022, Номер 30(10), С. 27636 - 27649

Опубликована: Ноя. 16, 2022

Abstract In agricultural ecosystems, bees are exposed to combinations of pesticides that may have been applied at different times. For example, visiting a flowering crop be chronically low concentrations systemic insecticides before bloom and then pulse fungicide, considered safe for bees, during bloom. this study, we simulate scenario under laboratory conditions with females the solitary bee, Osmia bicornis L. We studied effects chronic exposure neonicotinoid insecticide, Confidor® (imidacloprid) realistic concentration, (1 day) fungicide Folicur® SE (tebuconazole) field application rate. Syrup consumption, survival, four biomarkers: acetylcholinesterase (AChE), carboxylesterase (CaE), glutathione S-transferase (GST), alkaline phosphatase (ALP) were evaluated two time points. An integrated biological response (IBRv2) index was elaborated biomarker results. The had no impact on survival but temporarily reduced syrup consumption increased IBRv2 index, indicating potential molecular alterations. significantly neurological activity enzymes. co-exposure neonicotinoid-fungicide did not increase toxicity tested concentrations. AChE proved an efficient detection early both insecticide fungicide. Our results highlight importance assessing individual sub-individual endpoints better understand pesticide bees.

Язык: Английский

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Real-time monitoring of honeybee colony daily activity and bee loss rates can highlight the risk posed by a pesticide

The Science of The Total Environment, Год журнала: 2023, Номер 886, С. 163928 - 163928

Опубликована: Май 6, 2023

Язык: Английский

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Oral administration of phytochemicals protects honey bees against cognitive and motor impairments induced by the insecticide fipronil

PLoS ONE, Год журнала: 2024, Номер 19(3), С. e0300899 - e0300899

Опубликована: Март 25, 2024

Pollution produced by exposure to pesticides is a major concern for food security because the negative impacts on pollinators. Fipronil, an insecticide broadly used around globe has been associated with ongoing decline of bees. With characteristic neuroactive toxicodynamic, fipronil leads cognitive and motor impairments at sublethal dosages. Despite regional bans, multilevel strategies are necessary protection Recent evidence suggests that specific nutrients in diets bees may induce against insecticides. Here, we evaluated whether administration three phytochemicals, namely rutin, kaempferol p-coumaric acid provide Africanized honey bee Apis mellifera oral realistic dosages fipronil. We tested potential impairment induced phytochemicals learning, 24h memory, sucrose sensitivity control. found concentration-dependent learning control, but not memory or across window. also acid, mixture was innocuous generally offered Overall, our results indicate can be prophylactically protected insecticides via nutrition, providing alternative conflict between use As studied present nectar pollen, suggest nutritional composition, only its production, should considered when implementing conservation gardens co-cropping.

Язык: Английский

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The effect of pollen monodiets on fat body morphology parameters and energy substrate levels in the fat body and hemolymph of Apis mellifera L. workers

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Июль 2, 2024

Abstract Human activities associated with large-scale farms and the monocultures expose honey bees to one type of food. Moreover, there is an ongoing decline plant species producing pollen nectar in Europe. A poorly balanced diet affects a number processes occurring bee’s body. The fat body hemolymph are tissues that participate all them. Therefore, aim our study was determine effect hazel, pine, rapeseed, buckwheat, phacelia goldenrod on morphological parameters trophocytes, diameters cell nuclei oenocytes concentrations compounds involved energy metabolism (glucose, glycogen, triglycerides protein). In cage tests, were fed from first day life sugar candy (control group) or 10% addition 6 types. Hemolymph various locations collected 1-, 7- 14-day-old workers. Pollen produced by such as hazel pine increased glucose bee tissues, especially hemolymph. It can therefore be concluded they valuable sources (in form simple carbohydrates) which quickly used bees. plants blooming summer autumn proteins, glycogen body, third tergite. accumulation these length width trophocytes well enhanced metabolic activity, evidenced increasing diameter oenocyte nuclei. seems multi-pollen more for bees, but it important understand effects particular types context mono-diet. future, this will make possible produce mixtures ensure homeostasis apian

Язык: Английский

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Exposure of Larvae to Sublethal Thiacloprid Delays Bee Development and Affects Transcriptional Responses of Newly Emerged Honey Bees

Frontiers in Insect Science, Год журнала: 2022, Номер 2

Опубликована: Апрель 5, 2022

Understanding the cause of honey bee ( Apis mellifera ) population decline has attracted immense attention worldwide in recent years. Exposure to neonicotinoid pesticides is considered one most probable factors due physiological and behavioral damage they bees. However, influence thiacloprid, a relatively less toxic cyanogen-substituted form neonicotinoid, on L .) development not well studied. The toxicity sublethal thiacloprid larvae, pupae, emerging bees was assessed under laboratory conditions. We found that reduced survival rate larvae delayed which led lower bodyweight size. Furthermore, we identified differentially expressed genes involved metabolism immunity though RNA-sequencing newly-emerged adult GO enrichment analysis metabolism, catalytic activity, transporter activity. KEGG pathway indicated induced up-regulation related glutathione Toll-like receptor signaling pathway. Overall, our results suggest chronic can affect colonies by reducing delaying development.

Язык: Английский

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Nutritional resources modulate the responses of three bee species to pesticide exposure

Journal of Hazardous Materials, Год журнала: 2022, Номер 443, С. 130304 - 130304

Опубликована: Ноя. 1, 2022

Язык: Английский

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Phytochemicals, Probiotics, Recombinant Proteins: Enzymatic Remedies to Pesticide Poisonings in Bees

Environmental Science & Technology, Год журнала: 2023, Номер 58(1), С. 54 - 62

Опубликована: Дек. 21, 2023

The ongoing global decline of bees threatens biodiversity and food safety as both wild plants crops rely on bee pollination to produce viable progeny or high-quality products in high yields. Pesticide exposure is a major driving force for the decline, yet pesticide use remains unreconciled with conservation since studies demonstrate that continue be heavily exposed threatened by pesticides natural habitats. Pharmaceutical methods, including administration phytochemicals, probiotics (beneficial bacteria), recombinant proteins (enzymes) detoxification functions, show promise potential solutions mitigate poisonings. We discuss how these new methods can appropriately developed applied agriculture from biology ecotoxicology perspectives. As countless probiotics, exist, this Perspective will provide suggestive guidance accelerate development techniques directing research resources toward promising candidates. Furthermore, we practical limitations mentioned above realistic field applications propose recommendations overcome limitations. This builds framework allow researchers more efficiently order harmful impacts bees.

Язык: Английский

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Large-scale study investigating the effects of a tank mixture containing thiacloprid-prochloraz on honey bees (Apis mellifera)

Chemosphere, Год журнала: 2022, Номер 313, С. 137396 - 137396

Опубликована: Ноя. 25, 2022

Язык: Английский

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