Peanut genotypic stress tolerance imprints the rhizobacterial community patterns in acidic soil: Evidence from two years of field trials

Field Crops Research, Год журнала: 2025, Номер 322, С. 109771 - 109771

Опубликована: Янв. 29, 2025

Язык: Английский

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Long-Term Organic Fertilization Shields Soil Prokaryotes from Metal Stress While Mineral Fertilization Exacerbates it

Опубликована: Янв. 1, 2025

Язык: Английский

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Revealing the Bacteriome in Crop–Livestock–Forest Integration Systems in the Cerrado of MATOPIBA, Brazil

Forests, Год журнала: 2025, Номер 16(4), С. 626 - 626

Опубликована: Апрель 2, 2025

Sustainable agriculture relies on effective soil management, making it crucial to assess health, especially in areas of agricultural expansion, such as the Cerrado MATOPIBA region. strategies, integrated production systems (crop–livestock–forestry), are essential mitigate these impacts. However, little is known about effects microbial communities. The objective this study was evaluate bacterial communities associated with soils under different Soil samples from 0–10 cm depth layer were collected following land use systems: (i) native vegetation (NCV), (ii) Babassu forest (NPV), (iii) no-tillage soybean—regional standard system (NT-S), (iv) crop–forest integration (CFI), (v) crop–livestock (CLI), and (vi) livestock–forest (LFI). We measured chemical properties using next-generation sequencing (NGS) V3-V4 hypervariable region 16S rRNA gene. results revealed that (CFI, CLI, LFI) resulted changes properties, which contributed modulation most abundant taxa shows a positive correlation pH phosphorus content. Members Nitrosomonadaceae Sphingomonadaceae families more related containing forestry component (CFI LFI), while Bacillaceae evident (CLI).

Язык: Английский

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Comparison of RNA- and DNA-based 16S amplicon sequencing to find the optimal approach for the analysis of the uterine microbiome

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Май 16, 2025

Studies in humans and large animals indicate a relationship between the uterine microbiome composition endometrial receptivity. Despite many studies have been performed, analysis of remains challenging due to very low microbial biomass. other biological systems showed that RNA-based complements DNA-based results provides information about active bacteria sample. Thus, aim this study was establish highly sensitive specific 16S rRNA gene V3-V4 amplicon PCR from equine cytobrush samples compare DNA- analysis. An optimized protocol developed, which able detect less than 38 bacterial genome copies using DNA community standard. For generation starting cDNA, at least 10-fold higher sensitivity estimated compared approach. The comparison RNA isolated same as input for sequencing revealed much number sequence variants well taxonomic units This resulted significant differences alpha (Simpson, Chao1) beta diversity RNA- Differential abundance all levels. these differences, overall similar paired samples. Many were probably found Furthermore, is biased by copy numbers (1-21), ribosomes per cell, reflected approaches. In addition, suggested detecting cell-free and/or dead could be present Altogether, obtained advantages combined analysis, offering complementary valuable context fertility-related microbiome.

Язык: Английский

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Organic fertilization sustains high maize yields in acid soils through the cooperation of rhizosphere microbes and plants

Plant and Soil, Год журнала: 2025, Номер unknown

Опубликована: Май 22, 2025

Язык: Английский

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