The
effects
of
long-term
ammunition
pollution
on
microecological
characteristics
were
analyzed
to
formulate
microbial
remediation
strategies.
basic
physical
and
chemical
properties
the
water/sediment
(pH,
nitrate,
nitrite,
ammonium)
changed
significantly,
significant
differences
in
stable
isotope
ratios
N
O
(nitrate
nitrogen)
confirmed
degradability
TNT
by
indigenous
microorganisms
exposed
pollution.
Heavy
metals,
such
as
Pb,
Zn,
Cu,
Cd,
Cs,
Sb,
have
synergistic
toxic
ammunition-contaminated
sites,
significantly
decreased
diversity
richness
core
area.
However,
exposure
edge
area
induced
use
a
carbon
nitrogen
sources
for
life
activities
growth
development.
Bacteroidales
group
was
inhibited
contamination.
Mg
K
involved
internal
mechanism
transport,
enrichment,
metabolism
TNT.
Nine
strains
TNT-utilizing
microbes
screened
efficient
degradation
tolerance
typical
heavy
metals
(copper,
zinc
lead)
found
contaminated
compound
bacterial
agent
prepared
effective
repair
soil
improved
ecological
environment.
Water and Environment Journal,
Год журнала:
2023,
Номер
37(3), С. 538 - 548
Опубликована: Март 15, 2023
Abstract
Biodegradation
has
been
applied
to
remediate
explosives
contaminants,
and
bacteria
have
a
high
potential
for
the
degradation
of
explosives,
such
as
hexahydro‐1,3,5‐trinitro‐1,3,5‐triazine
(RDX)
2,4,6‐trinitrotoluene
(TNT).
The
present
study
aims
screen
characterize
explosive
biodegradable
Actinomycetes
from
water,
wastewater,
sludge.
isolates
were
recovered
80
environmental
samples
diverse
resources
in
contaminated
areas
Iran
identified
genus
species
levels
using
conventional
molecular
methods.
growth
rate
presence
pollutants
chromatography
was
used
determine
their
biodegradation
capability.
Twenty‐nine
(36.25%)
characterized
cultured
that
belonged
6
24
validated
species.
most
prevalent
isolated
Mycobacterium
with
11
(37.94%),
Rhodococcus
seven
(24.13%),
Nocardia
four
(13.8%),
Streptomyces
three
(10.33%).
Moreover,
our
results
showed
these
could
degrade
consume
50–80%
RDX
TNT
sole
carbon
energy
source.
In
conclusion,
we
explosive‐contaminated
RDX.
Hence,
seeking
screening
untapped
ecosystems
possess
unexplored
will
increase
chances
discovering
resident
microorganism
capable
degrading
application
bioremediation
process.
this
can
be
useful
intact
nature
eliminate
pollution,
which
is
one
major
problems
world.
Much
attention
has
been
paid
to
the
environmental
toxicity
and
ecological
risk
caused
by
cyclic
tetramethylene
tetranitramine
(HMX)
pollution
in
military
activity
sites.
In
this
study,
response
mechanism
of
alfalfa
plants
HMX
was
analyzed
from
aspects
photosynthetic
system,
micromorphology,
antioxidant
enzyme
mineral
metabolism,
secondary
metabolism.
Exposure
5
mg·L-1
resulted
a
significant
increase
leaf
N
content
drift
Fourier
transform
infrared
protein
peak
area.
Transmission
electron
microscopy
images
revealed
damage
root
system
subcellular
morphology,
but
plant
leaves
effectively
resisted
pressure,
parameters
essentially
maintained
steady-state
levels.
The
proline
decreased
significantly
23.1–47.2%,
reactive
oxygen
species
increased
1.66–1.80
fold.
roots
regulate
transport/absorption
many
elements
that
impart
stress
resistance,
Cu,
Mn,
Na
uptake
is
associated
with
metabolism
upregulated
general
exposure,
main
differences
appearing
lipids
lipid-like
molecules,
further
confirming
biofilm
structure.
causes
an
imbalance
energy
supply
oxidative
phosphorylation
generates
important
biomarkers
form
pyrophosphate
dihydrogen
phosphate.
Interestingly,
had
no
effect
on
basic
metabolic
networks
(i.e.,
glycolysis/gluconeogenesis
tricarboxylic
acid
cycle),
good
resistance.
Alfalfa
apparently
multiple
network
systems
resist/overcome
toxicity.
These
findings
provide
scientific
basis
for
improving
tolerance
understanding
mechanism.
The
effects
of
long-term
ammunition
pollution
on
microecological
characteristics
were
analyzed
to
formulate
microbial
remediation
strategies.
basic
physical
and
chemical
properties
the
water/sediment
(pH,
nitrate,
nitrite,
ammonium)
changed
significantly,
significant
differences
in
stable
isotope
ratios
N
O
(nitrate
nitrogen)
confirmed
degradability
TNT
by
indigenous
microorganisms
exposed
pollution.
Heavy
metals,
such
as
Pb,
Zn,
Cu,
Cd,
Cs,
Sb,
have
synergistic
toxic
ammunition-contaminated
sites,
significantly
decreased
diversity
richness
core
area.
However,
exposure
edge
area
induced
use
a
carbon
nitrogen
sources
for
life
activities
growth
development.
Bacteroidales
group
was
inhibited
contamination.
Mg
K
involved
internal
mechanism
transport,
enrichment,
metabolism
TNT.
Nine
strains
TNT-utilizing
microbes
screened
efficient
degradation
tolerance
typical
heavy
metals
(copper,
zinc
lead)
found
contaminated
compound
bacterial
agent
prepared
effective
repair
soil
improved
ecological
environment.
