Analytical and Bioanalytical Chemistry, Год журнала: 2024, Номер 416(5), С. 1149 - 1164

Опубликована: Янв. 4, 2024

Abstract The conditionally essential very-long-chain polyunsaturated fatty acids (VLC-PUFAs), such as eicosapentaenoic acid (EPA, C20:5 n-3), play a vital role in human nutrition. Their biological activity is thereby greatly influenced by the distinct glycerolipid molecule that they are esterified to. Here, microalgae differ from conventional source, fish oil, both quantity and distribution of VLC-PUFAs among glycerolipidome. Therefore, aim this study was to develop fast reliable one-dimensional high-performance thin-layer chromatography (HPTLC)-based method allows separation quantification main microalgal classes (e.g., monogalactosyldiacylglycerol (MGDG), sulfoquinovosyl diacylglycerol (SQDG), phosphatidylglycerol (PG)), well subsequent analysis their respective via gas (GC) coupled mass spectrometry (MS). Following optimization, validation carried out for 13 different lipid classes, based on International Conference Harmonization (ICH) guidelines. In HPTLC, linearity effective between 100 2100 ng, with limit 62.99 90.09 ng depending class, strong correlation coefficients ( R 2 > 0.995). recovery varied 93.17 108.12%, while inter-day precision measurements showed variation less than 8.85%, close detection. Applying crude extracts four EPA producing commercial interest, content assessed together FA band elution. results described precise accurate HPTLC offers possibility be used routinely follow variations class levels throughout strain screening, cultivation, or bioprocessing. Thus, additional quantitative analytical information complex lipidome can obtained, especially n-3 n-6 enriched fractions. Graphical

Язык: Английский