Polylactic acid micro/nanoplastic-induced hepatotoxicity: Investigating food and air sources via multi-omics

Environmental Science and Ecotechnology, Год журнала: 2024, Номер 21, С. 100428 - 100428

Опубликована: Май 3, 2024

Micro/nanoplastics (MNPs) are detected in human liver, and pose significant risks to health. Oral exposure MNPs derived from non-biodegradable plastics can induce toxicity mouse liver. Similarly, nasal cause airway dysbiosis mice. However, the hepatotoxicity induced by foodborne airborne biodegradable remains poorly understood. Here we show hepatotoxic effects of polylactic acid (PLA) through multi-omics analysis various biological samples mice, including gut, fecal, nasal, lung, blood samples. Our results that both PLA compromise liver function, disrupt serum antioxidant activity, pathology. Specifically, lead gut microbial dysbiosis, metabolic alterations serum, transcriptomic changes. Airborne affect lung microbiota, alter metabolites, transcriptomics. The Lachnospiraceae_NK4A136_group is a potential biomarker for MNP exposure, while unclassified

Язык: Английский

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The impact of microplastics polystyrene on the microscopic structure of mouse intestine, tight junction genes and gut microbiota

PLoS ONE, Год журнала: 2024, Номер 19(6), С. e0304686 - e0304686

Опубликована: Июнь 5, 2024

Microplastics, which are tiny plastic particles less than 5 mm in diameter, widely present the environment, have become a serious threat to aquatic life and human health, potentially causing ecosystem disorders health problems. The study aimed investigate effects of microplastics, specifically microplastics-polystyrene (MPs-PS), on structural integrity, gene expression related tight junctions, gut microbiota mice. A total 24 Kunming mice aged 30 days were randomly assigned into four groups: control male (CM), female (CF), PS-exposed (PSM), (PSF)(n = 6). There significant differences villus height, width, intestinal surface area, height crypt depth ratio (V/C) between PS group group(C) (p <0.05). Gene analysis demonstrated downregulation Claudin-1 , Claudin-2 Claudin-15 Occludin both duodenum jejunum < 0.05). Analysis microbial species using 16S rRNA sequencing indicated decreased diversity PSF group, as well reduced PSM at various taxonomic levels. Beta showed difference distribution C groups (R2 0.113, p<0.01), with this being more pronounced among females exposed MPs-PS. KEGG revealed enrichment differential mainly involved seven signaling pathways, such nucleotide metabolism(p<0.05). relative abundance transcriptional pathways was significantly increased for (p<0.01), while excretory system group(p<0.05). Overall findings suggest that MPs-PS exhibit notable sex-dependent impact mouse microbiota, stronger effect observed females; junction genes may be associated dysbiosis, particularly elevated levels Prevotellaceae .

Язык: Английский

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The gut microbiota, a key to understanding the health implications of micro(nano)plastics and their biodegradation

Microbial Biotechnology, Год журнала: 2022, Номер 16(1), С. 34 - 53

Опубликована: Ноя. 22, 2022

Abstract The effects of plastic debris on the environment and plant, animal, human health are a global challenge, with micro(nano)plastics (MNPs) being main focus. MNPs found so often in food chain that they provoking an increase intake. They have been detected most categories consumed foods, drinking water, even feces. Therefore, oral ingestion becomes source exposure to MNPs, gastrointestinal tract, primarily gut, constantly interacts these small particles. consequences remain unclear. However, current vivo studies vitro tract models shown several types sizes impact gut intestinal bacteria, affecting homeostasis. typical microbiome signature MNP is associated dysbiosis loss resilience, leads frequent pathogen outbreaks, local systemic metabolic disorders. Moreover, micro‐ nano‐plastic particles animal tissues accumulated evidence microbial degradation plastics/MNPs by bacteria insect microbiota raise issue whether make key contributions bio‐transformation ingested MNPs. Here, we discuss issues unveil complex interplay between microbiome. elucidation biological this interaction both host undoubtedly challenging. It expected biotechnology research could help decipher extent which microorganisms diversify MNP‐determinant species, mechanisms, enzymatic systems, as well become important understand our response provide background information inspire future holistic studies.

Язык: Английский

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Effects of microplastics and tetracycline on intestinal injury in mice

Chemosphere, Год журнала: 2023, Номер 337, С. 139364 - 139364

Опубликована: Июнь 27, 2023

Язык: Английский

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Oral exposure to polyethylene microplastics induces inflammatory and metabolic changes and promotes fibrosis in mouse liver.

Ecotoxicology and Environmental Safety, Год журнала: 2023, Номер 264, С. 115417 - 115417

Опубликована: Авг. 29, 2023

Accumulating evidence shows widespread contamination of water sources and food with microplastics. Although the liver is one main sites bioaccumulation within human body, it still unclear whether microplastics produce damaging effects. In particular, hepatic consequences ingesting polyethylene (PE) in mammals are unknown. this study, female mice were fed contaminated 36 116 µm diameter PE microbeads at a dosage 100 µg/g for 6 9 weeks. Mice exposed to each type microbead, or co-exposed 2 types microbeads. Mouse showed altered levels genes involved uptake, synthesis, β-oxidation fatty acids. Ingestion disturbed detoxification response, promoted oxidative imbalance, increased inflammatory foci cytokine expression, enhanced proliferation liver. Since relative expression stellate cell marker Pdgfa collagen deposition following exposure, we assessed effect ingestion mouse model CCl4-induced fibrosis that dietary exposure exacerbated fibrogenesis. These findings provide first demonstration adverse effects highlight need further health risk assessment humans.

Язык: Английский

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The hepatotoxicity assessment of micro/nanoplastics: A preliminary study to apply the adverse outcome pathways

The Science of The Total Environment, Год журнала: 2023, Номер 902, С. 165659 - 165659

Опубликована: Июль 29, 2023

Язык: Английский

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Hazardous potential assessment of airborne and foodborne polyvinyl alcohol and polyhydroxyalkanoates nanoplastics: Comparison with polyvinyl chloride nanoplastics

Chemical Engineering Journal, Год журнала: 2024, Номер 485, С. 150122 - 150122

Опубликована: Март 1, 2024

Язык: Английский

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Polystyrene microplastics promote intestinal colonization of Aeromonas veronii through inducing intestinal microbiota dysbiosis

Journal of Hazardous Materials, Год журнала: 2024, Номер 469, С. 133976 - 133976

Опубликована: Март 8, 2024

Язык: Английский

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Mongolian medicine formulae Ruda-6 alleviates indomethacin-induced gastric ulcer by regulating gut microbiome and serum metabolomics in rats

Journal of Ethnopharmacology, Год журнала: 2023, Номер 314, С. 116545 - 116545

Опубликована: Май 17, 2023

Ruda-6 (RD-6), a typical traditional Mongolian medicine formulae consisting of 6 herbs, has been traditionally used in treating gastric disorders. Even though it shown to protect against ulcers (GU) animal models, the gut microbiome and serum metabololite-related mechanisms that prevent GU are not well understood.This study was conducted evaluate gastroprotective mechanism RD-6 associated with alteration metabolic profiles rats.RD-6 (0.27, 1.35 2.7 g/kg) or ranitidine (40 mg/kg) were orally administered rats for three weeks before induction ulcer using indomethacin (30 mg/kg, single oral dose). The index, area, H&E staining, levels TNF-α, iNOS, MPO MDA quantified inhibitory effects RD-6. Then, 16S rRNA gene sequencing combined LC-MS profiling performed investigate effect on microbiota metabolites rats. Moreover, spearman analysis calculate correlation coefficient between different metabolites.RD-6 inhibited lesion damage caused by rats, decreased index 50.29% (p < 0.05), reduced tissue. Additionally, reshaped diversity microbial composition, reversed bacteria including [Eubacterium]_xylanophilum group, Sellimonas, Desulfovibrio, UCG-009, increased Aquamicrobium induction. Furthermore, regulated amino acids organic acids, these affected involved taurine hypotaurine metabolism tryptophan metabolism. Spearman revealed perturbed closely related changes differential metabolites.In view results, present suggests ameliorating via modulating intestinal their metabolites.

Язык: Английский

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Combined exposure to polyvinyl chloride and polystyrene microplastics induces liver injury and perturbs gut microbial and serum metabolic homeostasis in mice

Ecotoxicology and Environmental Safety, Год журнала: 2023, Номер 267, С. 115637 - 115637

Опубликована: Ноя. 1, 2023

A variety of microplastics (MPs) have become ubiquitous environmental pollutants, leading to inevitable human contact and health impacts. Most previous research has explored the toxic effects a single type MPs exposure. However, co-exposure both common types MPs, polyvinyl chloride (PVC) polystyrene (PS) on mammals not been explored. Here, adult mice were exposed PS-PVC (1.0 µm PS 2.0 PVC at concentration 0.5 mg/day) for 60 days. The results showed that co-exposure-induced hepatotoxicity was evidenced by liver histopathological changes, release inflammatory cytokines, activation oxidative stress. Moreover, intestinal mucosal barrier damaged after treatment. 16S rRNA gene sequencing reported there marked shift in gut microbial structure accompanied decreased relative abundances probiotics, such as Clostridium, Lachnospiraceae_UCG-006, Desulfovibrio, Clostridiales_unclassified Ruminococcaceae_unclassified increased conditional pathogen abundances, Erysipelatoclostridium. Furthermore, triglyceride (TG) total cholesterol (TCH) expression levels serum co-exposure. Serum metabolomics analysis 717 differential metabolites found positive- negative-ion modes, including 476 up-regulated 241 down-regulated, mainly enriched butyrate metabolism, thiamine phenylacetate metabolism. In addition, remarked changes microbiota metabolic profiles closely related hepatic injuries These provided new insights into through gut-liver axis risks should be paid more attention humans.

Язык: Английский

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