Enhanced detection of mpox virus in wastewater using a pre-amplification approach: A pilot study informing population-level monitoring of low-titer pathogens

The Science of The Total Environment, Год журнала: 2023, Номер 903, С. 166230 - 166230

Опубликована: Авг. 11, 2023

Язык: Английский

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Enabling quantitative comparison of wastewater surveillance data across methods through data standardization without method standardization

The Science of The Total Environment, Год журнала: 2024, Номер 953, С. 176073 - 176073

Опубликована: Сен. 7, 2024

Язык: Английский

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Quantitative association of SARS-CoV-2 in wastewater and clinically confirmed cases in different areas of the Tokyo 2020 Olympic and Paralympic Village

The Science of The Total Environment, Год журнала: 2025, Номер 960, С. 178209 - 178209

Опубликована: Янв. 1, 2025

International mass gathering events, such as the Olympic and Paralympic Games, face risk of cross-border transmission infectious diseases. We previously reported that wastewater-based epidemiology (WBE), which has attracted attention a COVID-19 surveillance tool, was implemented in Tokyo 2020 Village to gain comprehensive understanding incidence village. In present study, we explored quantitative association wastewater viral load clinically confirmed cases various areas From July 14 through September 8, 2021, 360 passive samples 329 grab were collected from seven distinct within village manholes examined for SARS-CoV-2 RNA by Efficient Practical virus Identification System with Enhanced Sensitivity (EPISENS) methods. The detection rates showed significant (P < 0.001, φ = 0.32, chi-square test), sampling showing higher positive rate. Based on Receiver Operating Characteristic (ROC) curve analysis cases, most sensitive cutoff point judged be limit quantification (LOQ) three-day samples. Under this optimal condition, sensitivity specificity 0.78 0.40, respectively. study demonstrated effectiveness building-level based cases. Wastewater can powerful tool monitor diseases among temporary residents, tourists participants international provided proper analytical methods are employed.

Язык: Английский

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The development of COPMAN-Air: A highly sensitive method for detecting SARS-CoV-2 in air

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Фев. 18, 2025

Several studies have successfully detected SARS-CoV-2 in air samples; however, most of these, the focus was on validating collection method, and there no report development a virus-detection method. In this study, to detect viruses samples more sensitively than conventional detection methods, we applied COPMAN, highly sensitive method using wastewater samples, develop COPMAN-Air. Briefly, with extremely low amount viral RNA is efficiently via three reaction steps: RT, preamplification, qPCR, as COPMAN. We evaluated COPMAN-Air from fever clinic for COVID-19 patients. demonstrated higher rate compared methods: 22 (95.7%) vs. 14 (60.9%) out 23 samples. Additionally, positive correlation (r=0.70) found between by number confirmed cases, suggesting that could estimate SARS-CoV-2-positive individuals given space based quantitative values Surveillance systems pathogens are expected be valuable assessing infected implementation public health measures.

Язык: Английский

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Association between confirmed COVID-19 cases at hospitals and SARS-CoV-2 levels in municipal wastewater during the pandemic and endemic phases

Environment International, Год журнала: 2025, Номер 197, С. 109342 - 109342

Опубликована: Фев. 20, 2025

Язык: Английский

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Development of COPMAN-Air method for high-sensitivity detection of SARS-CoV-2 in air

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Апрель 24, 2025

Abstract Several studies have successfully detected SARS-CoV-2 in air samples. However, most of these focused on validating the collection method, and there was no report development a virus detection method. In this study, to detect viruses samples with greater sensitively than conventional methods, we utilized COPMAN, highly sensitive method originally used for wastewater We applied COPMAN samples, thereby developing COPMAN-Air. Briefly, efficiently detects extremely low levels viral RNA via three reaction steps: RT, preamplification, qPCR, as it is performed COPMAN. evaluated COPMAN-Air using from fever clinic COVID-19 patients. demonstrated higher rate compared detecting 22 out 23 (95.7%) vs. 14 (60.9%). Additionally, positive correlation ( r = 0.70) between amount by number confirmed cases, suggesting that could estimate SARS-CoV-2-positive individuals given space based quantitative values Surveillance systems airborne pathogens are expected be valuable estimating infected guiding implementation public health measures.

Язык: Английский

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Development of a Rapid, Cost-Effective, and Automatable Virus Concentration from Wastewater Using a Coagulant with Magnetic Materials

ACS ES&T Water, Год журнала: 2025, Номер unknown

Опубликована: Май 16, 2025

Язык: Английский

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Molecular epidemiology of human sapovirus based on the surveillance of wastewater and patients with acute gastroenteritis in Osaka, Japan

The Science of The Total Environment, Год журнала: 2025, Номер 985, С. 179622 - 179622

Опубликована: Май 28, 2025

Wastewater-based epidemiology has recently emerged as a promising tool for determining the prevalence of infectious diseases in community. In present study, human sapoviruses (HuSaVs) detected wastewater collected weekly from January 2023 to March 2024 were analyzed using qPCR and next-generation sequencing (NGS), results compared with those clinical surveillance samples obtained patients acute gastroenteritis (AGE) Osaka Prefecture, Japan. The detection trend HuSaV agreed data that genomes increased cold season. surveillance, five genotypes detected, including GI.1, GI.2, GII.3, GII.5, GV.1. GI.2 was not surveillance. phylogenetic analysis, VP1 gene sequences positioned close proximity samples. It is suggested reflect status ongoing infection Interestingly, GV.1 less frequently than GI.1 samples, but predominantly at nearly all time points, accounting up 94.5 % reads November 2023. particular, 2023, most outbreaks pediatric AGE cases attributable higher proportion GI.1. Wastewater shown complement former might be able cover asymptomatic carriers. Our demonstrate importance systematic epidemiological analyses HuSaV.

Язык: Английский

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Tracking community infection dynamics of COVID-19 by monitoring SARS-CoV-2 RNA in wastewater, counting positive reactions by qPCR

The Science of The Total Environment, Год журнала: 2023, Номер 904, С. 166420 - 166420

Опубликована: Авг. 21, 2023

Язык: Английский

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Tracking the effects of the COVID-19 pandemic on viral gastroenteritis through wastewater-based retrospective analyses

The Science of The Total Environment, Год журнала: 2023, Номер 905, С. 166557 - 166557

Опубликована: Авг. 24, 2023

The COVID-19 pandemic possibly disrupted the circulation and seasonality of gastroenteritis viruses (e.g., Norovirus (NoV), Sapovirus (SaV), group A rotavirus (ARoV), Aichivirus (AiV)). Despite growing application wastewater-based epidemiology (WBE), there remains a lack sufficient investigations into actual impact on prevalence viruses. In this study, we measured NoV GI GII, SaV, ARoV, AiV RNA concentrations in 296 influent wastewater samples collected from three treatment plants (WWTPs) Sapporo, Japan between October 28, 2018 January 12, 2023 using highly sensitive EPISENS™ method. detection ratios SaV ARoV after May 2020 (SaV: 49.8 % (134/269), ARoV: 57.4 (151/263)) were significantly lower than those before April 93.9 (31/33), 97.0 (32/33); SaV: p < 3.5×10-7, 1.5×10-6). Furthermore, despite comparable (88.5 %, 23/26) during (66.7 80/120) (p = 0.032), GII revealed significant decrease onset 1.5×10-7, Cliff's delta 0.72). sporadically detected (24.7 8/33) (6.5 17/263), whereas was consistently (100 33/33) throughout study period (95.8 252/263). WBE results demonstrated influence countermeasures viruses, with variations observed magnitude their across different types These epidemiological findings highlight that hygiene practices implemented to prevent infections may also be effective for controlling providing invaluable insights public health units development disease management guidelines.

Язык: Английский

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