The
widespread
use
of
engineered
nanomaterials
(ENMs)
has
expanded
faster
than
our
understanding
their
toxicity,
prompting
research
into
the
biological
responses
against
exposure
to
such
materials.
Genomics
and
transcriptomics
have
been
extensively
used
evaluate
effects
various
ENMs.
By
determining
gene
activities,
these
studies
provided
valuable
information
infer
how
cells
respond
toxicological
many
application
mass
spectrometry
(MS)-based
omics
tools,
as
proteomics,
lipidomics,
metabolomics,
offer
post-genomic
perspectives
what
cellular
processes
are
altered.
Individually,
technologies
revealed
proteome,
lipidome,
metabolome
landscape
upon
Together,
approaches
demonstrate
ENM-induced
adaptation
in
a
broad
range
at
multiple
levels
complexity
ENM–cell
interactions.
As
result,
integrating
layers
MS-based
data
is
trending
complement
genomics
data.
In
this
chapter,
we
discuss
applications
tools
for
comprehensive
systems-level
characterization
induced
by
nanomaterials.
Analytical Chemistry,
Год журнала:
2023,
Номер
unknown
Опубликована: Дек. 19, 2023
Microflow
liquid
chromatography
interfaced
with
mass
spectrometry
(μLC-MS/MS)
is
increasingly
applied
for
high-throughput
profiling
of
biological
samples
and
has
been
proven
to
have
an
acceptable
trade-off
between
sensitivity
reproducibility.
However,
lipidomics
applications
are
scarce.
We
optimized
a
μLC-MS/MS
system
utilizing
1
mm
inner
diameter
×
100
column
coupled
triple
quadrupole
spectrometer
establish
sensitive,
high-throughput,
robust
single-shot
workflow.
Compared
conventional
methods,
we
achieve
∼4-fold
increase
in
response,
facilitating
quantification
351
lipid
species
from
single
iPSC-derived
cerebral
organoid
during
15
min
LC-MS
analysis.
Consecutively,
injected
303
over
∼75
h
prove
the
robustness
reproducibility
microflow
separation.
As
proof
concept,
analysis
Alzheimer's
disease
patient-derived
iPSC
reveals
differential
metabolism
depending
on
APOE
phenotype
(E3/3
vs
E4/4).
separation
proves
be
environmentally
friendly
cost-effective
method
as
it
reduces
consumption
harmful
solvents.
Also,
data
demonstrate
robust,
in-depth,
performance
enable
routine
clinical
or
biomedical
applications.
Future Medicinal Chemistry,
Год журнала:
2023,
Номер
15(7), С. 579 - 582
Опубликована: Апрель 1, 2023
Organoids
provide
us
an
opportunity
to
understand
how
diseases
affect
cellular
physiology,
human
tissues
or
organs.
They
are
indespensible
tools
for
biomaterial
toxicity
analysis,
drug
discovery
and
regenerative
medicine.
Endocrine Regulations,
Год журнала:
2024,
Номер
58(1)
Опубликована: Янв. 1, 2024
Nanographene
oxide
(nGO)
nanoparticles
(NPs)
have
unique
properties
and
are
widely
used
in
various
fields,
including
biomedicine.
These
NPs,
however,
also
exhibit
toxic
ef-fects
therefore,
the
understanding
of
molecular
mechanism
nGO
toxicity
is
very
im-portant
mainly
for
nanomedicine,
especially
cancer
therapy.
This
study
aimed
to
examine
impact
NPs
on
expression
genes
associated
with
endoplasmic
reticulum
(ER)
stress,
proliferation,
cancerogenesis
both
normal
human
astrocytes
U87MG
glioblas-toma
cells.
The
widespread
use
of
engineered
nanomaterials
(ENMs)
has
expanded
faster
than
our
understanding
their
toxicity,
prompting
research
into
the
biological
responses
against
exposure
to
such
materials.
Genomics
and
transcriptomics
have
been
extensively
used
evaluate
effects
various
ENMs.
By
determining
gene
activities,
these
studies
provided
valuable
information
infer
how
cells
respond
toxicological
many
application
mass
spectrometry
(MS)-based
omics
tools,
as
proteomics,
lipidomics,
metabolomics,
offer
post-genomic
perspectives
what
cellular
processes
are
altered.
Individually,
technologies
revealed
proteome,
lipidome,
metabolome
landscape
upon
Together,
approaches
demonstrate
ENM-induced
adaptation
in
a
broad
range
at
multiple
levels
complexity
ENM–cell
interactions.
As
result,
integrating
layers
MS-based
data
is
trending
complement
genomics
data.
In
this
chapter,
we
discuss
applications
tools
for
comprehensive
systems-level
characterization
induced
by
nanomaterials.
