Application of eDNA Metabarcoding Technology to Monitor the Health of Aquatic Ecosystems

Water, Год журнала: 2025, Номер 17(8), С. 1109 - 1109

Опубликована: Апрель 8, 2025

Environmental DNA (eDNA) is isolated from environmental samples. It distinctly different genomic DNA, which extracted directly biological specimens. eDNA metabarcoding technology a novel surveillance tool combining and second-generation high-throughput sequencing technology. Different conventional approaches biomonitoring techniques, (eMT) has many advantages, such as promising timeliness accuracy, lower time consumption, low cost, thus widely used in ecological monitoring, including that rivers, lakes, oceans, soils, sediments. As tool, supplements the evaluation of qualities by monitoring both diversity aquatic biology communities target species. In addition, it essential to understand limitations practical applications. features high efficiency, providing indicators health allowing for indirect estimation impact extent water pollution with respect ecosystems. provides new insights environment protection.

Язык: Английский

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Sampling eDNA at outflows from artificial drainage systems: what is the potential to monitor landscape degradation?

Environmental Monitoring and Assessment, Год журнала: 2025, Номер 197(5)

Опубликована: Апрель 26, 2025

Язык: Английский

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Single metabarcoding multiplex captures community‐level freshwater biodiversity and beyond

Environmental DNA, Год журнала: 2024, Номер 6(1)

Опубликована: Янв. 1, 2024

Abstract Cost‐effective and accurate quantification of biodiversity is important for conservation, resource management, forecasting. Traditional monitoring approaches have relied on direct observations, remote sensing, mark‐recapture techniques, providing insights into species ecology the impact pollution climate change indicator species. However, these techniques are typically low throughput, expensive, can be invasive. In addition, they cannot detect cryptic diversity biased toward that leave identifiable remains. DNA‐based methods, such as metabarcoding or single marker gene assays, enabled high throughput screening a wide range taxonomic groups, including ones without well‐preserved When compared with traditional resolve diversity, do not require specialist skills, non‐invasive. although comparatively cheaper than approaches, expensive when applied at community‐level assays amplified sequenced independently. Multilocus in which multiple markers reaction desirable to deliver assessments cost‐effective manner. Yet, uncommon because technical challenges may lead biases downstream analyses, index hopping unbalanced representation groups. Here, we developed highly multiplexed protocol combines early pooling genes target broad groups taxon‐specific tube reaction. This step followed by up 384 samples per locus ( N = 15,636 samples) unique dual‐indexed sequencing adapters run. approach dramatically reduces costs lowers need input DNA compromising output quality. We optimized multiplex assay lake freshwater sediment benchmarked from other environmental matrices, demonstrating its application river marine communities.

Язык: Английский

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Advancing fish diversity monitor in degraded marine ecosystem with environmental DNA approach: Unveiling hidden riches

Ecological Indicators, Год журнала: 2024, Номер 160, С. 111893 - 111893

Опубликована: Март 1, 2024

Comprehending fish diversity through multiple dimensions is a crucial aspect in advancing our understanding of populations and promoting sustainable fisheries as well conservation. Traditional research on has been limited to non-genetic methods. The advent environmental DNA (eDNA) technology enables simultaneous examination both genetic aspects diversity. In this study, we evaluated the ability eDNA capture multidimensional northern South China Sea, with emphasis phylogenetic effect change geographical location across different seasons was also investigated. analysis species accumulation curves revealed that observed richness approached an asymptote, achieving 94.7% 89.5% predicted values during summer winter, respectively. Alpha taxonomic (species, 225 vs. species, 102), functional (FRic, 0.51 FRic, 0.19), (PD, 26.27 PD, 15.81) were found be higher compared factors significantly influencing these diversities seasons. Regarding three beta diversity, replacement made greater relative contribution total taxonomic, functional, than difference. These effects primarily influenced by water environment (e.g., NO2−, SiO32−, surface temperature) longitude latitude) winter. This study provides evidence supporting feasibility utilizing techniques uncover dimensions, specific its intrinsic characteristics, which hold substantial value for protection scientific administration fishery resources.

Язык: Английский

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How land use affects freshwater zooplankton communities: a global overview

Hydrobiologia, Год журнала: 2024, Номер unknown

Опубликована: Июнь 14, 2024

Язык: Английский

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Ecological restoration enhanced the stability of epiphytic microbial food webs of submerged macrophytes: Insights from predation characteristics of epiphytic predators

The Science of The Total Environment, Год журнала: 2024, Номер 948, С. 174547 - 174547

Опубликована: Июль 9, 2024

Язык: Английский

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eDNA Biomonitoring of Macroinvertebrate Communities for the Bioassessment of a River’s Ecological Status

Water, Год журнала: 2023, Номер 15(2), С. 308 - 308

Опубликована: Янв. 11, 2023

Environmental DNA (eDNA) becomes a promising technology for macroinvertebrate monitoring worldwide. In recent decades, with increasing humanization processes, such as water pollution and habitat fragmentation, the richness abundance of macroinvertebrates show dramatic decline, which is particularly evident in tropical or subtropical rivers. The high-throughput rapid species’ survival ecological status their habitats are relevant to river management. Here, we used eDNA detect Dongjiang River—a typical Southern China, assess status, based on datasets. Our data showed total 640 OTUs detected by technology, belonging three phyla, five classes, 13 orders, 33 families 71 genera macroinvertebrates, these taxa had 36.6% coverage rate historical at genus level. traditional quality index (WQI) that upstream River were mainly levels I~II, middle stream II~III, downstream IV~V. eDNA-based biotic indices almost same findings, is, overall was: > reaches downstream. Overall, this study provides important datasets technical support ecosystem management

Язык: Английский

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eDNA-based diversity and multitrophic network reveal the effects of land use and pollutants on the subtropical Dongjiang River systems

Environmental Pollution, Год журнала: 2023, Номер 334, С. 122157 - 122157

Опубликована: Июль 14, 2023

Язык: Английский

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Embracing eDNA and machine learning for taxonomy-free microorganisms biomonitoring to assess the river ecological status

Ecological Indicators, Год журнала: 2023, Номер 155, С. 110948 - 110948

Опубликована: Сен. 15, 2023

Currently implemented biomonitoring and bioassessment are an indispensable part of river management worldwide. Although DNA-based is a promising tool for biodiversity detection, substantial effort still exists about how DNA-derived microorganisms' datasets can be used routine bioassessment. Here, we analyzed microorganisms (i.e., bacteria microbial eukaryotes) collected from the Dongjiang River, to develop Metabarcoding-eDNA Index (MEI) using taxonomy-free strategy based on supervised machine learning, identify comparability new MEI index with two traditional water pollution indices trophic state index, TSI, quality WQI) assessing ecological status. First, our data showed that rich diversity in rivers was detected by eDNA technology, yet 40–90% OTUs were unknown across taxa because deficiency reference databases inability annotate. Second, combining quantile regression ca. 90% reliably classified into different groups corresponding TSI WQI. Third, had significant consistent correlations (Rbacteria2 = 0.74; Reukaryotes2 0.69) WQI 0.64; 0.53). Overall, incorporating eDNA-derived this study further validates reliability robustness biomonitoring, supporting application status assessment.

Язык: Английский

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Seasonal changes of plankton community and its influencing factors in subtropical coastal marine areas revealed by eDNA-based network analysis

Frontiers in Marine Science, Год журнала: 2024, Номер 11

Опубликована: Сен. 5, 2024

Introduction Coastal marine ecosystems are among the most ecologically and socio-economically dynamic regions on Earth, study of eukaryotic planktonic communities is an important aspect aquatic ecology. It essential to monitor plankton biodiversity identify influencing factors in order measure human effects coastal waters help contribute ecosystem preservation. Environmental DNA (eDNA) metabarcoding analysis advanced toolfor detecting presence organisms, which has revolutionary significance for monitoring real-time detection wild environments. Methods In this study, eDNA was used seasonal changes subtropical northern South China Sea, explore how various trophic groups affect complexity stability species network. Results The results showed that summer interaction network looser had reduce biodiversitycompared spring As part community, abundance diversity algae reflect interactions between different influence environmental dominant species, particularly temperature nutrients. addition, producer set basis indirectly affected stability, while consumer its flexible predation strategies may directly lead differences across seasons. There were close correlations interaction, diversity. Discussion This can us understand functions throughout year complex from a multiple level perspective, provide effective methods sustainable resource utilization ecological protection future.

Язык: Английский

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