Glyphosate Effects on Growth and Biofilm Formation in Bee Gut Symbionts and Diverse Associated Bacteria

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Март 21, 2024

Abstract Biofilm formation is a common adaptation enabling bacteria to thrive in various environments and withstand external pressures. In the context of host-microbe interactions, biofilms play vital roles establishing microbiomes associated with animals plants are used by opportunistic microbes facilitate proliferation within hosts. Investigating biofilm dynamics, composition, responses environmental stressors crucial for understanding microbial community assembly regulation health disease. this study, we explore independent gut colonization vitro abilities core members honey bee ( Apis mellifera ) microbiota. Additionally, assess impact glyphosate, widely herbicide antimicrobial properties, glyphosate-based formulation on growth symbionts as well other biofilm-forming diverse plants. Our results demonstrate that several strains bacterial species can independently colonize gut, which probably depends their ability form biofilms. Furthermore, glyphosate exposure has varying effects formation. These findings imply specific impacts both ecological host health-related implications. Importance Biofilms essential communities establish environments. microbiota member Snodgrassella alvi forms biofilms, potentially aiding establishment promoting interactions host. show members, including Bifidobacterium , Bombilactobacillus Gilliamella Lactobacillus also We then examine disrupts microbiota, formation, ranging from inhibition enhancement, reflecting observations beneficial or pathogenic Thus, may influence shaping surfaces impacting outcomes.

Язык: Английский

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Direct and indirect effects of land use on microbiomes of trap-nesting solitary bee larvae and nests

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Авг. 28, 2024

The global decline in biodiversity and insect populations highlights the urgent to conserve ecosystem functions, such as plant pollination by solitary bees. Human activities, particularly agricultural intensification, pose significant threats these essential services. Changes land use alter resource nest site availability, pesticide exposure other factors impacting richness, diversity, health of bee species. In this study, we investigated yet another facet currently less well context: Microbial communities associated with wild bees play crucial roles larval development, metabolism, ­immunity overall health. However, drivers dynamics healthy microbiome are still poorly understood, especially regarding direct indirect effects on diversity composition microbial communities. We examined bacterial offspring materials Megachilid trap-nesting bee, O. bicornis,along a gradient intensification 16S rRNA gene metabarcoding. Given that landscape composition, climatic conditions, food resources known influence compositions species, hypothesized changes would available for material collection thereby affecting microbiomes their environments. anticipated reduced altered increased which is decrease number resources, including pool floral soil bacteria surrounding environment. As expected, observed shifts nests across varying degrees intensity, differing management types availability flowers. Shannon (larval pollen provision, enclosure) guts decreased increasing intensity. pupae remained unaffected, indicating reorganization during metamorphosis, not significantly influenced resources. Our findings provide new insights into shaping environmental transmission microbiomes. This understanding comprehending impacts intensive developing strategies mitigate effects.

Язык: Английский

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Toxicity of glyphosate herbicides formulated for Africanized Apis mellifera Linnaeus, 1758 (Hymenoptera: Apidae)

Ecotoxicology and Environmental Safety, Год журнала: 2024, Номер 287, С. 117247 - 117247

Опубликована: Ноя. 1, 2024

Initially, products based on glyphosate (GLY) were considered non-toxic or slightly toxic to bees. Still, recent research has shown that these can cause mortality trigger sublethal effects in insects. Roundup Transorb R® (RT) is one of the GLY-based formulations sold Brazil. It used several crops, and studies are required its toxicity honey Thus, objective this work was evaluate, under laboratory conditions, lethal RT for adult workers (foragers) Africanized A. mellifera. For this, two bioassays carried out with The experimental design completely randomized, consisting five treatments (T0 - control, T25 25 % GLY, T50 50 T75 75 T100 GLY 100 recommended dose). as follows: (1) Acute oral topical exposure, evaluating mortality, flight capacity, vertical displacement, locomotion (in latter only contamination), repetitions 10 bees per repetition; (2) Chronic exposure via route spraying, assessing both contamination routes damage midgut epithelium thickness when contaminated route, composed replicates 20 replicate. results showed chronic increase honeybee their epithelium. In addition, acutely exposed orally, had reduced walking ability. did not affect other evaluated parameters. it concluded RT-formulated survival, morphology, behavior

Язык: Английский

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Effect of an insecticide, fungicide and plant growth regulator and their mixture on the survival of the springtail Folsomia candida and the potential reduction of toxicity by vitamins

Environmental Toxicology and Pharmacology, Год журнала: 2024, Номер 112, С. 104588 - 104588

Опубликована: Ноя. 13, 2024

Язык: Английский

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Nanomaterials for managing abiotic and biotic stress in the soil–plant system for sustainable agriculture

Environmental Science Nano, Год журнала: 2024, Номер unknown

Опубликована: Янв. 1, 2024

An optimum application of NPs can mitigate biotic and abiotic stresses, but overuse cause phytotoxicity. Research on the use for plant stress is demonstrating promising benefits.

Язык: Английский

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