Response of antioxidation and immunity to combined influences of pH and ammonia nitrogen in the spotted babylon (Babylonia areolata) DOI Creative Commons

Ruixia Ding,

Ruifu Yang, Zhengyi Fu

и другие.

Heliyon, Год журнала: 2024, Номер 10(8), С. e29205 - e29205

Опубликована: Апрель 1, 2024

Spotted babylon were exposed to three different pH levels (7.0, 8.0 and 9.0) four concentrations of ammonia nitrogen (0.02, 1.02, 5.10 10.20 mg/L) in seawater determine their acute toxicity physiological responses environmental fluctuation. The study evaluated antioxidant enzymes: catalase (CAT), alkaline, superoxide dismutase (SOD), peroxidase (POD) glutathione (GSH-PX), two immunoenzymes: acid phosphatase (ACP) (AKP). Over time, the immunoenzyme activity was significantly affected by concentration. After being nitrogen, spotted showed signs unresponsiveness external stimuli, reduced vitality, slow movement, an inability maintain upright position. exhibited a trend increasing then decreasing GSH-PX, CAT, SOD activities adapt changing environment enhance its immunity. On contrary, POD ACP initially, followed over time AKP gradual increase with time. combined effect found be stronger than either factor alone. interaction between increased enzymes, induced oxidative stress, ability babylon's non-specific immune system reverse it. Thus, reverse-back higher when stress dual or single-factor stresses. results will establish theoretical basis for analyzing risk multiple factors babylon, also enrich basic information about shellfish system.

Язык: Английский

Selenium-enriched Lactiplantibacillus plantarum alleviates alkalinity stress-induced selective hepatic insulin resistance in common carp DOI

Xinghua Che,

Xinchi Shang,

Weixu

и другие.

International Journal of Biological Macromolecules, Год журнала: 2025, Номер 305, С. 141204 - 141204

Опубликована: Фев. 20, 2025

Язык: Английский

Процитировано

3
Effects of saline and alkaline stresses on the survival, growth, and physiological responses in juvenile mandarin fish (Siniperca chuatsi) DOI
Yan Li,

Pengcheng Gao,

Kai Zhou

и другие.

Aquaculture, Год журнала: 2024, Номер 591, С. 741143 - 741143

Опубликована: Май 27, 2024

Язык: Английский

Процитировано

14
Acute alkalinity stress induces functional damage and alters immune metabolic pathways in the gill tissue of spotted scat (Scatophagus argus) DOI
Lei Yang,

Adili Abudu,

Kecheng Zhu

и другие.

Aquaculture, Год журнала: 2025, Номер unknown, С. 742186 - 742186

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

1
Effects of prometryn on oxidative stress, immune response and apoptosis in the hepatopancreas of Eriocheir sinensis (Crustacea: Decapoda) DOI Creative Commons
Peng Huang, Jinliang Du, Liping Cao

и другие.

Ecotoxicology and Environmental Safety, Год журнала: 2023, Номер 262, С. 115159 - 115159

Опубликована: Июнь 24, 2023

Prometryn, a triazine pesticide product used to control weed growth, poses high risk aquatic organisms in the environment. Several toxicological evaluations have been performed on bony fish and shrimp exposed prometryn. However, there no reports conducted toxic mechanism of prometryn with regard Eriocheir sinensis. In this study, our research evaluated effects via vitro vivo toxicity tests E. Firstly, we estimated exposure sinensis, then constructed 6 h transcriptional profile an enrichment analysis. To further reveal prometryn, hepatopancreas (hepatopancreatic cells) was analyzed for antioxidant, immune lipid-metabolism-related enzymes, antioxidant- apoptosis-related gene expression, histopathology TUNEL. From results, determined that 96 h-LD50 70.059 mg/kg, using RNA-seq, identified 933 differentially expressed genes (DEGs), which were mainly enriched amino fatty acid metabolism cell-fate-determination-related signaling pathway. The results biochemical assays showed could significantly decrease activities/levels CAT, SOD, GSH, AKP ACP, reduce levels T-AOC, TG, TCH, C3 C4, increase MDA content. addition, expression Nrf2, GSTs HO-1 first upregulated downregulated increasing time. Histopathology damaged structure cells induced apoptosis, suggesting PI3K-Akt pathway may be involved damage process (PI3K, PDK Akt whereas Bax upregulated), leading their apoptosis. above indicated cause injury through oxidative stress, induce cell disrupt lipid damage. This study provided useful data understanding evaluating crustacea.

Язык: Английский

Процитировано

22
Ginger polysaccharide alleviates the effects of acute exposure to carbonate in crucian carp (Carassius auratus) by regulating immunity, intestinal microbiota, and intestinal metabolism DOI Creative Commons
Xianwei Meng, Liang Luo, Zhigang Zhao

и другие.

Ecotoxicology and Environmental Safety, Год журнала: 2024, Номер 273, С. 116127 - 116127

Опубликована: Фев. 22, 2024

Alkaline stress poses a significant challenge to the healthy growth of fish. Ginger polysaccharide (GP) is one main active substances in ginger and has pharmacological effects, such as anti-oxidation immune regulation. However, physiological regulatory mechanism GP addition diet on alkalinity crucian carp remains unclear. This study aimed investigate potential protective effects dietary antioxidant capacity, gene expression levels, intestinal microbiome, metabolomics exposed carbonate (NaHCO3). The CK group (no supplementation) COG (NaHCO3 no were set up. GPCS 0.4% was stressed for seven days. Based these data, significantly increased activities total capacity (T-AOC), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-PX), acid phosphatase (ACP), alkaline (AKP) under (p < 0.05) decreased activity malon dialdehyde (MDA) 0.05). restored GSH-PX, ACP, AKP levels. levels tumor necrosis factor β (TGF-β), factor-alpha (TNF-α), interferon-gamma (IFN-γ), interleukin 8 (IL-8) genes decreased, determination kappa-B (NF-κB) 10 (IL-10) 16 S rRNA high-throughput sequencing, improved changes microbial diversity structural composition caused by NaHCO3 exposure. In particular, relative abundance Proteobacteria Bacteroidetes Actinobacteria. metabolic response guts studied using LC/MS. Compared group, had 64 different metabolites enriched pathways, including lipid metabolism, nucleotide carbohydrate metabolism. feed can promote galactose metabolism provide an energy supply carp, thus alleviating damage induced stress. conclusion, mitigate regulating function, flora, carp. These findings novel idea studying salt-alkali tolerance adding feed.

Язык: Английский

Процитировано

8
Integrated characterizations of intestinal bacteria and transcriptomics revealed the acute stress response to carbonate alkalinity in white shrimp Penaeus vannamei DOI

Zule Song,

Kui Li, Kejun Li

и другие.

Fish & Shellfish Immunology, Год журнала: 2024, Номер 146, С. 109420 - 109420

Опубликована: Фев. 6, 2024

Язык: Английский

Процитировано

5
H2O2-Induced Oxidative Stress Responses in Eriocheir sinensis: Antioxidant Defense and Immune Gene Expression Dynamics DOI Creative Commons

Qinghong He,

Wenrong Feng, Xue Chen

и другие.

Antioxidants, Год журнала: 2024, Номер 13(5), С. 524 - 524

Опубликована: Апрель 26, 2024

, a key species in China's freshwater aquaculture, is threatened by various diseases, which were verified to be closely associated with oxidative stress. This study aimed investigate the response of

Язык: Английский

Процитировано

5
Effects of sulfate on survival, osmoregulation and immune inflammation of mud crab(Scylla paramamosain) under low salt conditions DOI
Yun Hu, Gao Gao, Kangxiang Qin

и другие.

Aquaculture, Год журнала: 2024, Номер 590, С. 741029 - 741029

Опубликована: Май 10, 2024

Язык: Английский

Процитировано

5
Reducing Dietary Protein Content by Increasing Carbohydrates Is More Beneficial to the Growth, Antioxidative Capacity, Ion Transport, and Ammonia Excretion of Nile Tilapia (Oreochromis niloticus) under Long-Term Alkalinity Stress DOI Creative Commons
Wei Liu, Chang Xu, Li Zhao

и другие.

Aquaculture Nutrition, Год журнала: 2023, Номер 2023, С. 1 - 15

Опубликована: Ноя. 16, 2023

Alkalinity stress is the main experienced by aquatic animals in saline-alkali water, which hinders aquaculture development and utilization of water resources. The two-factor (2 × 3) test was adopted to study influence dietary protein carbohydrate ratios on energy metabolism Nile tilapia (Oreochromis niloticus) under different alkalinity levels. Three diets with protein-carbohydrate (P27/C35, P35/C25, P42/C15) were fed fish cultured freshwater (FW, 1.3 mmol/L carbonate alkalinity) or alkaline (AW, 35.7 for 50 days. Ambient decreased growth performance. Although ambient caused oxidative enhanced ion transport ammonia tilapia, P27/C35 diet showed better adaptability than other two water. Further metabolomic analysis that upregulated all pathways enriched this cope stress. Under stress, exhibited pyruvate purine compared P42/C15 diet. This indicated could significantly decrease performance cause osmotic regulation. However, reducing content increasing carbohydrates weaken improve performance, transport, long-term hyperalkaline exposure.

Язык: Английский

Процитировано

11
Response of the terrestrial isopod Porcellio scaber to lipopolysaccharide challenge after microplastic and insecticide exposure DOI Creative Commons

Andraž Dolar,

Tina Petrišič,

Damjana Drobne

и другие.

The Science of The Total Environment, Год журнала: 2024, Номер 925, С. 171698 - 171698

Опубликована: Март 16, 2024

The exposure of organisms to microplastics could compromise their ability cope with other environmental stressors, such as infections. In this context, we investigated the effects a 14-day terrestrial isopod Porcellio scaber tire particles in soil (1.5 % w

Язык: Английский

Процитировано

4