CRISPR/Cas12a-based biosensors for environmental monitoring and diagnostics

Environmental Technology & Innovation, Год журнала: 2024, Номер 34, С. 103625 - 103625

Опубликована: Апрель 4, 2024

Contaminants, such as nucleic acids or toxic small molecules, threaten both human health and ecosystems when they infiltrate the environment. The precise highly sensitive identification of contaminants holds paramount importance across diverse domains, including safeguarding food integrity, facilitating clinical diagnostics, monitoring environmental conditions. Traditional methodologies, encompassing spectroscopy, chromatography, sequencing, metagenomics, have conventionally served pivotal roles in detection processes. Nevertheless, these methods encountered recurring challenges related to sensitivity, specificity, portability. This review focuses on groundbreaking CRISPR/Cas12-based biosensors. These biosensors leverage incredible precision programmability CRISPR/Cas system recognize specific targets. Here, we comprehensively assess fundamental mechanisms that enable detection, ranging from guide RNA design collateral cleavage. versatility CRISPR/Cas12 becomes evident through their applications. applications encompass medical safety, monitoring. transition conventional ultimately represents a significant milestone contaminant detection. By incorporating molecular biology, nanotechnology, bioinformatics, potential reshape landscape water CRIPSR-Cas diagnostics is transformative technology paves way for safer healthier future environment life.

Язык: Английский

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Aptamer-based point-of-care-testing for small molecule targets: From aptamers to aptasensors, devices and applications

TrAC Trends in Analytical Chemistry, Год журнала: 2023, Номер 169, С. 117408 - 117408

Опубликована: Ноя. 2, 2023

Язык: Английский

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CRISPR-powered biosensing platform for quantitative detection of alpha-fetoprotein by a personal glucose meter

Sensors and Actuators B Chemical, Год журнала: 2023, Номер 390, С. 133994 - 133994

Опубликована: Май 18, 2023

Язык: Английский

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A ratiometric electrochemical aptasensor for sensitive detection of kanamycin in food based on entropy-driven strand displacement reaction

Food Control, Год журнала: 2024, Номер 161, С. 110390 - 110390

Опубликована: Фев. 20, 2024

Язык: Английский

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CRISPR-Cas12a-driven aptasensor for sensitive detection of alternariol by using a personal glucose meter

Talanta, Год журнала: 2025, Номер 286, С. 127496 - 127496

Опубликована: Янв. 4, 2025

Язык: Английский

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Fluorescence detection of the genetic risk factor ApoE4 gene associate with Alzheimer’s disease based on a CRISPR-Cas12a system

Microchemical Journal, Год журнала: 2025, Номер unknown, С. 112831 - 112831

Опубликована: Янв. 1, 2025

Язык: Английский

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Sensitive and Portable Signal Readout Strategies Boost Point-of-Care CRISPR/Cas12a Biosensors

ACS Sensors, Год журнала: 2023, Номер 8(11), С. 3988 - 4007

Опубликована: Окт. 23, 2023

Point-of-care (POC) detection is getting more and attention in many fields due to its accuracy on-site test property. The CRISPR/Cas12a system endowed with excellent sensitivity, target identification specificity, signal amplification ability biosensing because of unique trans-cleavage ability. As a result, lot research has been made develop CRISPR/Cas12a-based biosensors. In this review, we focused on readout strategies summarized recent sensitivity-improving fluorescence, colorimetric, electrochemical signaling. Then introduced novel portability-improving based lateral flow assays (LFAs), microfluidic chips, simplified instruments, one-pot design. the end, also provide our outlook for future development

Язык: Английский

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Modeling and Simulation of Photonic Crystal Sensor for Drinking Water Quality Monitoring

Progress In Electromagnetics Research C, Год журнала: 2024, Номер 140, С. 85 - 91

Опубликована: Янв. 1, 2024

Photonics crystal sensors, sensitive to light, play a crucial role in discerning minute alterations material's refractive index, finding widespread application, such as monitoring drinking water quality.Our objective is fashion sensor based on 2D photonics structure and scrutinize optical transformations induced by variations the bacteria's index light traverses structure.Leveraging Rsoft's simulation capabilities, we assessed transmission spectra, observing shifts their consequential impact signal's frequency wavelength within structure.The simulations unequivocally demonstrate that fluctuations significantly affect wavelength.Consequently, study underscores efficacy of Rsoft-designed bacterial presence contaminated water, achieving an average sensitivity 834.344 nm/RIU.In conclusion, establishes success crafted with Rsoft software detecting bacteria polluted water.By during traversal, are translated into discernible wavelength, facilitating effective detection.

Язык: Английский

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Phosphorothioate-modified G-quadruplex as a signal-on dual-mode reporter for CRISPR/Cas12a-based portable detection of environmental pollutants

Analytica Chimica Acta, Год журнала: 2024, Номер 1308, С. 342649 - 342649

Опубликована: Апрель 25, 2024

Язык: Английский

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Multiple gRNAs-assisted CRISPR/Cas12a-based portable aptasensor enabling glucometer readout for amplification-free and quantitative detection of malathion

Analytica Chimica Acta, Год журнала: 2025, Номер 1341, С. 343662 - 343662

Опубликована: Янв. 13, 2025

Язык: Английский

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