The Science of The Total Environment,
Год журнала:
2024,
Номер
948, С. 174981 - 174981
Опубликована: Июль 23, 2024
Areas
of
dense
population
congregation
are
prone
to
experience
respiratory
virus
outbreaks.
We
monitored
wastewater
and
clinic
patients
for
the
presence
viruses
on
a
large,
public
university
campus.
Campus
sewer
systems
were
in
16
locations
using
next
generation
sequencing
over
22
weeks
2023.
During
this
period,
we
detected
surge
human
adenovirus
(HAdV)
levels
wastewater.
Hence,
initiated
clinical
surveillance
at
an
on-campus
from
presenting
with
acute
infection.
From
whole
genome
123
throat
and/or
nasal
swabs
collected,
identified
outbreak
HAdV,
specifically
HAdV-E4
HAdV-B7
genotypes
overlapping
time.
The
temporal
dynamics
proportions
HAdV
found
corroborated
infections.
tracked
specific
single
nucleotide
polymorphisms
(SNPs)
sequences
showed
that
they
arose
signals
concordant
time
presentation,
linking
community
transmission
outbreak.
This
study
demonstrates
how
wastewater-based
epidemiology
can
be
integrated
ambulatory
healthcare
settings
monitor
areas
outbreaks
provide
health
guidance.
Water Research,
Год журнала:
2024,
Номер
254, С. 121338 - 121338
Опубликована: Фев. 18, 2024
Quantitative
polymerase
chain
reaction
(PCR)
and
genome
sequencing
are
important
methods
for
wastewater
surveillance
of
severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV-2).
The
reverse
transcription-droplet
digital
PCR
(RT-ddPCR)
is
a
highly
sensitive
method
quantifying
SARS-CoV-2
RNA
in
samples
to
track
the
trends
viral
activity
levels
but
cannot
identify
new
variants.
It
also
takes
time
develop
PCR-based
assays
targeting
variants
interest.
Whole
(WGS)
can
be
used
monitor
known
variants,
it
generally
not
quantitative.
Several
short-read
techniques
expensive
might
experience
delayed
turnaround
times
when
outsourced
due
inadequate
in-house
resources.
Recently,
portable
nanopore
system
offers
an
affordable
real-time
wastewater.
This
technology
has
potential
enable
swift
response
disease
outbreaks
without
relying
on
clinical
results.
In
addressing
concerns
related
rapid
accurate
variant
analysis,
both
RT-ddPCR
were
employed
emergence
was
conducted
at
23
sewer
maintenance
hole
sites
five
treatment
plants
Michigan
from
2020
2022.
2020,
dominated
by
parental
(20A,
20C
20G),
followed
20I
(Alpha,
B.1.1.7)
early
2021
Delta
concern
(VOC)
late
2021.
For
year
2022,
Omicron
dominated.
Nanopore
validate
suspected
cases
that
initially
undetermined
assays.
concordance
rate
between
identifying
clade-level
76.9%.
Notably,
instances
disagreement
two
most
prominent
identification
We
showed
with
N
gene
concentrations
>104
GC/100ml
as
measured
improve
recovery
coverage
depth
using
MinION
device.
better
detecting
key
spike
protein
mutations
A67V,
del69-70,
K417N,
L452R,
N501Y,
N679K,
R408S
(p-value
<0.05)
compared
sequencing.
suggested
should
coordinated
where
preliminary
quantification
confirmatory
detection
or
reported
here
adopted
reliable
analysis
community
level
public
health
response.
Heliyon,
Год журнала:
2024,
Номер
10(13), С. e33873 - e33873
Опубликована: Июнь 29, 2024
A
focus
on
water
quality
has
intensified
globally,
considering
its
critical
role
in
sustaining
life
and
ecosystems.
Wastewater,
reflecting
societal
development,
profoundly
impacts
public
health.
Wastewater-based
epidemiology
(WBE)
emerged
as
a
surveillance
tool
for
detecting
outbreaks
early,
monitoring
infectious
disease
trends,
providing
real-time
insights,
particularly
vulnerable
communities.
WBE
aids
tracking
pathogens,
including
viruses,
sewage,
offering
comprehensive
understanding
of
community
health
lifestyle
habits.
With
the
rise
global
COVID-19
cases,
gained
prominence,
aiding
SARS-CoV-2
levels
worldwide.
Despite
advancements
treatment,
poorly
treated
wastewater
discharge
remains
threat,
amplifying
spread
water-,
sanitation-,
hygiene
(WaSH)-related
diseases.
WBE,
serving
complementary
surveillance,
is
pivotal
community-level
viral
infections.
However,
there
untapped
potential
to
expand
surveillance.
This
review
emphasizes
importance
link
between
health,
highlighting
need
further
integration
into
management.
International Journal of Hygiene and Environmental Health,
Год журнала:
2024,
Номер
259, С. 114379 - 114379
Опубликована: Апрель 15, 2024
Wastewater
analysis
can
serve
as
a
source
of
public
health
information.
In
recent
years,
wastewater-based
epidemiology
(WBE)
has
emerged
and
proven
useful
for
the
detection
infectious
diseases.
However,
insights
from
wastewater
treatment
plant
do
not
allow
small-scale
differentiation
within
sewer
system
that
is
needed
to
analyze
target
population
under
study
in
more
detail.
Small-scale
WBE
offers
several
advantages,
but
there
been
no
systematic
overview
its
application.
The
aim
this
scoping
review
provide
comprehensive
current
state
knowledge
on
diseases,
including
methodological
considerations
A
database
search
was
conducted,
considering
only
peer-reviewed
articles.
Data
analyses
included
quantitative
summary
qualitative
narrative
synthesis.
Of
2130
articles,
we
278,
most
which
were
published
since
2020.
studies
analyzed
at
building
level
(n
=
203),
especially
healthcare
110)
educational
facilities
80),
neighborhood
scale
86).
main
analytical
parameters
viruses
178),
notably
SARS-CoV-2
161),
antibiotic
resistance
(ABR)
biomarkers
99),
often
by
polymerase
chain
reaction
(PCR),
with
DNA
sequencing
techniques
being
less
common.
terms
sampling
techniques,
active
dominated.
frequent
lack
detailed
information
specification
selection
criteria
characterization
sites
identified
concern.
conclusion,
based
large
number
studies,
overarching
strategic
aspects
WBE.
An
enabling
environment
requires
inter-
transdisciplinary
sharing
across
countries.
Promoting
adoption
will
benefit
common
international
conceptualization
approach,
standardized
internationally
accepted
terminology.
particular,
development
good
practices
different
warranted.
This
includes
establishment
guidelines
local
sub-sewersheds,
transparent
reporting
ensure
comparability
results.
Analytical Chemistry,
Год журнала:
2024,
Номер
96(22), С. 9167 - 9176
Опубликована: Май 18, 2024
The
detection
of
virus
RNA
in
wastewater
has
been
established
as
a
valuable
method
for
monitoring
Coronavirus
disease
2019.
Carbon
nanomaterials
hold
potential
application
separating
owing
to
their
effective
adsorption
and
extraction
capabilities.
However,
carbon
have
limited
separability
under
homogeneous
aqueous
conditions.
Due
the
stabilities
nanostructure,
it
is
challenge
efficiently
immobilize
them
onto
magnetic
beads
separation.
Here,
we
develop
porous
agarose
layered
graphene
oxide
(GO)
nanocomposite
that
prepared
by
agglutinating
ferroferric
(Fe
Research Square (Research Square),
Год журнала:
2025,
Номер
unknown
Опубликована: Март 25, 2025
AbstractBackground
Severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV-2)
is
an
ongoing
threat
to
global
health.
Wastewater-based
surveillance
(WBS)
has
proven
be
important
tool
for
tracking
the
dissemination
of
SARS-CoV-2
variants
concern
(VOCs)
in
community.
In
Canada,
metagenomic
analysis
aircraft
wastewater
was
adopted
at
early
stage
pandemic
track
importation
emerging
into
country.
However,
need
determine
presence
sublineages
meant
that
sampling
methods
utilized
were
not
adequately
validated.
Here,
we
compared
two
different
genomic
VOCs
sewage
samples.
Methods
Eighty-eight
composite
samples
collected
over
nine
weeks
using
both
autosampler
and
passive
torpedo
samplers
same
location.
nucleic
acid
quantified
RT-qPCR.
RNA
extracted
sequenced
with
MiniSeq
system
tiled-amplicon
sequencing
approach
ARTIC
V4.1
primer
sets.
Raw
reads
preprocessed
mutations,
lineages,
other
sequence
metrics
from
compared.
Results
The
yielded
comparable
viral
load
by
RT-qPCR,
but
produced
higher
genome
coverage
relative
samplers.
Omicron
lineages
identified
differed
method.
BQ.1*
BA.5.2*,
which
predominant
clinical
time,
as
dominant
sampler,
respectively.
Additionally,
captured
diversity
abundance
VOCs,
including
(XBB*
CH.1*
lineages),
well
more
clinically
relevant
mutations
(S:K444T,
T22942A,
S:R346T)
sampler.
Overall,
thepassive
concordant
results
measuring
RT-qPCR
wastewater.
Conclusions
Taken
together,
our
suggest
underestimation
These
data
can
used
optimize
approaches
PLOS Global Public Health,
Год журнала:
2025,
Номер
5(4), С. e0004439 - e0004439
Опубликована: Апрель 24, 2025
Wastewater-based
environmental
surveillance
(ES)
has
been
demonstrated
to
provide
an
early
warning
signal
predict
variant-driven
waves
of
pathogens
such
as
severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV-2).
Our
study
evaluated
the
potential
cost-effectiveness
ES
for
SARS-CoV-2
compared
with
clinical
testing
alone.
We
used
Covasim
agent-based
model
COVID-19
simulate
disease
transmission
hypothetical
populations
in
Blantyre,
Malawi,
and
Kathmandu,
Nepal.
simulated
introduction
a
new
immune-escaping
variant
over
6
months
estimated
health
outcomes
(cases,
deaths,
disability-adjusted
life
years
[DALYs])
economic
impact
when
using
trigger
moderate
proactive
behavioral
intervention
(e.g.,
increased
use
masks,
social
distancing)
by
policymakers
versus
no
hence
delayed
reactive
intervention.
Costs
considered
included
ES,
testing,
treatment,
productivity
loss
entire
population
due
implementation
calculated
incremental
ratios
these
local
willingness-to-pay
thresholds:
$61
Malawi
$249
performed
sensitivity
analyses
evaluate
key
assumptions
on
results.
are
reported
2022
US
dollars.
estimate
that
if
were
implemented,
approximately
600
DALYs
would
be
averted
Blantyre
300
six-month
period.
Considering
system
costs,
was
cost-effective
cost-saving
Kathmandu.
Cost-effectiveness
highest
settings
low
surveillance,
high
severity,
effectiveness.
However,
from
societal
perspective,
may
not
depending
magnitude
population-wide
losses
associated
threshold.
or
tool
perspective
linked
effective
public
response.
From
however,
length
its
consequences
make
cost-effective.
Implementing
multiple
improve
cost-effectiveness.
medRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Март 28, 2024
Areas
of
dense
population
congregation
are
prone
to
experience
respiratory
virus
outbreaks.
We
monitored
wastewater
and
clinic
patients
for
the
presence
viruses
on
a
large,
public
university
campus.
Campus
sewer
systems
were
in
16
locations
using
next
generation
sequencing
over
22
weeks
2023.
During
this
period,
we
detected
surge
human
adenovirus
(HAdV)
levels
wastewater.
Hence,
initiated
clinical
surveillance
at
an
on-campus
from
presenting
with
acute
infection.
From
whole
genome
123
throat
and/or
nasal
swabs
collected,
identified
outbreak
HAdV,
specifically
HAdV-E4
HAdV-B7
genotypes
overlapping
time.
The
temporal
dynamics
proportions
HAdV
found
corroborated
infections.
tracked
specific
single
nucleotide
polymorphisms
(SNPs)
sequences
showed
that
they
arose
signals
concordant
time
presentation,
linking
community
transmission
outbreak.
This
study
demonstrates
how
wastewater-based
epidemiology
can
be
integrated
ambulatory
healthcare
settings
monitor
areas
outbreaks
provide
health
guidance.
medRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Фев. 3, 2024
Abstract
Wastewater
can
play
a
vital
role
in
infectious
disease
surveillance,
especially
underserved
communities
where
it
reduce
the
equity
gap
to
larger
municipalities.
However,
using
wastewater
surveillance
predictive
manner
remains
challenge.
We
tested
if
detecting
SARS-CoV-2
predict
outbreaks
rural
communities.
Under
CDC
National
Surveillance
program,
we
monitored
several
Idaho
(USA).
While
high
daily
variations
viral
load
made
real-time
interpretation
difficult,
SEIR
model
could
factor
out
data
noise
and
forecast
start
of
Omicron
outbreak
five
six
cities
that
were
sampled
soon
after
quantities
increased
wastewater.
For
one
city,
an
11
days
before
reported
clinical
cases
began
increase.
An
epidemiological
modeling
approach
transform
how
epidemiologists
use
provide
public
health
guidance
on
diseases
