The Science of The Total Environment,
Год журнала:
2024,
Номер
948, С. 174981 - 174981
Опубликована: Июль 23, 2024
Areas
of
dense
population
congregation
are
prone
to
experience
respiratory
virus
outbreaks.
We
monitored
wastewater
and
clinic
patients
for
the
presence
viruses
on
a
large,
public
university
campus.
Campus
sewer
systems
were
in
16
locations
using
next
generation
sequencing
over
22
weeks
2023.
During
this
period,
we
detected
surge
human
adenovirus
(HAdV)
levels
wastewater.
Hence,
initiated
clinical
surveillance
at
an
on-campus
from
presenting
with
acute
infection.
From
whole
genome
123
throat
and/or
nasal
swabs
collected,
identified
outbreak
HAdV,
specifically
HAdV-E4
HAdV-B7
genotypes
overlapping
time.
The
temporal
dynamics
proportions
HAdV
found
corroborated
infections.
tracked
specific
single
nucleotide
polymorphisms
(SNPs)
sequences
showed
that
they
arose
signals
concordant
time
presentation,
linking
community
transmission
outbreak.
This
study
demonstrates
how
wastewater-based
epidemiology
can
be
integrated
ambulatory
healthcare
settings
monitor
areas
outbreaks
provide
health
guidance.
Water Environment Research,
Год журнала:
2024,
Номер
96(4)
Опубликована: Апрель 1, 2024
Abstract
The
recent
SARS‐CoV‐2
outbreak
yielded
substantial
data
regarding
virus
fate
and
prevalence
at
water
reclamation
facilities
(WRFs),
identifying
influential
factors
as
natural
decay,
adsorption,
light,
pH,
salinity,
antagonistic
microorganisms.
However,
no
studies
have
quantified
the
impact
of
these
in
full
scale
WRFs.
Utilizing
a
mass
balance
approach,
we
assessed
decay
other
mechanisms
on
genetic
marker
removal
during
reclamation,
through
use
sludge
wastewater
loading
estimates.
Results
indicated
negligible
markers
P/PT
(primary
effluent
(PE)
p
value:
0.267;
preliminary
primary
treatment
(P/PT)
accumulation
0.904;
thickened
(TPS)
0.076)
indicating
contribution
toward
P/PT.
Comparably,
adsorption
decomposition
was
found
to
be
dominant
pathway
for
(thickened
waste
activated
(TWAS)
log
9.75
10
GC/day);
however,
estimation
could
carried
out
due
high
detections
TWAS.
Practitioner
Points
approach
suggested
that
are
compared
with
(
0.904).
During
(P/PT),
higher
viral
load
remained
(14.16
GC/day)
TPS
(13.83
statistical
difference
observed
0.280)
indicting
adsorption/decomposition
most
probably
did
not
occur.
In
secondary
(ST),
TWAS
were
consistently
detected
(13.41
(SE),
longer
HRT
potential
presence
extracellular
polymeric
substance‐containing
enriched
biomass
enabled
adsorption/decomposition.
Estimations
total
solids
volatile
affinity
different
between
sampling
locations
<0.0001).
The Science of The Total Environment,
Год журнала:
2024,
Номер
948, С. 174981 - 174981
Опубликована: Июль 23, 2024
Areas
of
dense
population
congregation
are
prone
to
experience
respiratory
virus
outbreaks.
We
monitored
wastewater
and
clinic
patients
for
the
presence
viruses
on
a
large,
public
university
campus.
Campus
sewer
systems
were
in
16
locations
using
next
generation
sequencing
over
22
weeks
2023.
During
this
period,
we
detected
surge
human
adenovirus
(HAdV)
levels
wastewater.
Hence,
initiated
clinical
surveillance
at
an
on-campus
from
presenting
with
acute
infection.
From
whole
genome
123
throat
and/or
nasal
swabs
collected,
identified
outbreak
HAdV,
specifically
HAdV-E4
HAdV-B7
genotypes
overlapping
time.
The
temporal
dynamics
proportions
HAdV
found
corroborated
infections.
tracked
specific
single
nucleotide
polymorphisms
(SNPs)
sequences
showed
that
they
arose
signals
concordant
time
presentation,
linking
community
transmission
outbreak.
This
study
demonstrates
how
wastewater-based
epidemiology
can
be
integrated
ambulatory
healthcare
settings
monitor
areas
outbreaks
provide
health
guidance.
