Two
pilot-scale
two-stage
anoxic/oxic
membrane
bioreactors
were
operated
at
different
dissolved
oxygen
(DO)
levels
to
evaluate
the
nitrogen
removal
performances
for
treating
landfill
sludge
dewatering
liquid
(SDL)
via
partial
nitrification
(PN)
and
denitrification.
Compared
low
DO
system
(2.0
–
3.0
mg/L),
high
(5.0
6.0
mg/L)
exhibited
efficient
PN
denitrification,
with
nitrite
accumulation
efficiency
(98.5%)
(98.0%).
The
removed
mainly
(53.8%)
simultaneous
denitrification
(45.1%).
Kinetic
inhibition
tests
verified
optimal
pH
5.0
mg/L
8.5.
Enriched
nitrifying
(Nitrosomonas)
denitrifying
bacteria
(Thiopseudomonas,
Pseudomonas,
Truepera)
in
environment
accomplished
process.
Metagenomic
analysis
showed
that
enriched
functional
genes
such
as
amoA/B/C,
hao,
nirK/S,
while
was
unsuitable
of
ammonia
wastewater.
Frontiers in Microbiology,
Год журнала:
2024,
Номер
14
Опубликована: Янв. 29, 2024
The
landfill
is
a
cheap
way
of
solid
waste
management
in
developing
countries.
majority
landfills
are
non-sanitary
and
work
as
open
garbage
dumping
sites
pose
threats
to
public
environmental
health.
Therefore,
an
in-depth
understanding
the
chemistry
microbiology
imperative
develop
right
policies
for
management.
In
current
study,
we
investigated
three
Indian
using
culture-based
culture-independent
molecular
approaches.
Our
data
indicate
that
nature
varies
from
site
terms
chemistry,
pollutants,
pathogens.
We
also
enriched
cultivated
methanogens
optimized
medium
constructed
two
high-quality
draft
genomes
microbiomes
metagenome-assembled
genome
phylogenomic
study
one
showed
highest
93%
sequence
similarity
with
members
Methanomassiliicoccaceae
was
always
Acholoplasma
Anaerohalosphaera
lusitana
.
Despite
all
efforts,
did
not
isolate
it
pure
culture
hypothesized
cultivation
some
not-yet-cultured
methanogen,
presence
other
organisms
plays
important
role,
their
syntrophic
interaction
must
be
discerned
its
successful
future.
Co-cultivation
amino
acid-degrading
indicates
co-culture
can
assist
boosting
growth
methanogens.
addition,
our
indicated
leachate
contains
heavy
load
pollutants
treatment
before
discharge
or
use
irrigation
biofertilizer.
Two
pilot-scale
two-stage
anoxic/oxic
membrane
bioreactors
were
operated
at
different
dissolved
oxygen
(DO)
levels
to
evaluate
the
nitrogen
removal
performances
for
treating
landfill
sludge
dewatering
liquid
(SDL)
via
partial
nitrification
(PN)
and
denitrification.
Compared
low
DO
system
(2.0
–
3.0
mg/L),
high
(5.0
6.0
mg/L)
exhibited
efficient
PN
denitrification,
with
nitrite
accumulation
efficiency
(98.5%)
(98.0%).
The
removed
mainly
(53.8%)
simultaneous
denitrification
(45.1%).
Kinetic
inhibition
tests
verified
optimal
pH
5.0
mg/L
8.5.
Enriched
nitrifying
(Nitrosomonas)
denitrifying
bacteria
(Thiopseudomonas,
Pseudomonas,
Truepera)
in
environment
accomplished
process.
Metagenomic
analysis
showed
that
enriched
functional
genes
such
as
amoA/B/C,
hao,
nirK/S,
while
was
unsuitable
of
ammonia
wastewater.
