Wastewater-based genomic surveillance of SARS-CoV-2 in vulnerable communities in Mumbai

The Indian Journal of Medical Research, Год журнала: 2025, Номер 160, С. 570 - 577

Опубликована: Янв. 8, 2025

Background & objectives The global impact of COVID-19, with over 45 million cases and 533,300 deaths in India alone, necessitates effective surveillance methods. Traditional approaches face challenges detecting pre-symptomatic asymptomatic cases, prompting the exploration wastewater-based epidemiology (WBE). This study focuses on Mumbai's vulnerable slums, aiming to assess potential WBE as an alternative method. Methods Genomic SARS-CoV-2 was conducted settings (slums) for 11 months (August 2022 June 2023). Wastewater samples from open drains sewage treatment plants were correlated reported COVID-19 city. Early detection emerging viral variants seasonal variations load explored. Results Correlations identified between wastewater early occurring three weeks before clinical diagnoses, underscoring utility WBE. sequencing provided insights into variants, identifying shifts predominant variants. Seasonal showed higher concentrations summer monsoon, potentially associated accelerated droplet evaporation droplet-based transmission during mid-summer monsoon. Interpretation conclusions Wastewater-based emerges a cost-effective rapid warning system, providing crucial virus behaviour evolution. Particularly significant countries like India, aids outbreak monitoring targeted interventions. integration emphasizes its importance comprehensive pandemic monitoring, establishing it integral component public health strategies worldwide.

Язык: Английский

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Persistence of human respiratory viral RNA in wastewater-settled solids

Applied and Environmental Microbiology, Год журнала: 2024, Номер 90(4)

Опубликована: Март 19, 2024

Wastewater-based epidemiology has emerged as a valuable tool for monitoring respiratory viral diseases within communities by analyzing concentrations of nucleic-acids in wastewater. However, little is known about the fate virus Two important processes that may modulate their wastewater they move from household drains to point collection include sorption or partitioning solids and degradation. This study investigated decay kinetics genomic seven human viruses, including severe acute syndrome coronavirus 2 (SARS-CoV-2), syncytial (RSV), (HCoV)-OC43, HCoV-229E, HCoV-NL63, rhinovirus (HRV), influenza A (IAV), well pepper mild mottle (PMMoV) solids. Viruses (except PMMoV) were spiked into followed 50 days at three different temperatures (4°C, 22°C, 37°C). Viral RNA decayed following first-order with rate constants

Язык: Английский

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Survival of viruses in water microcosms

The Science of The Total Environment, Год журнала: 2025, Номер 963, С. 178416 - 178416

Опубликована: Янв. 15, 2025

Язык: Английский

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Impedance-based method for the quantification of infectious SARS-CoV-2

Journal of Virological Methods, Год журнала: 2025, Номер unknown, С. 115110 - 115110

Опубликована: Янв. 1, 2025

Язык: Английский

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Measuring SARS-CoV-2 RNA in Bangkok wastewater treatment plants and estimating infected population after fully opening the country in 2023, Thailand

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Март 20, 2025

Wastewater-based epidemiology (WBE) has been employed for monitoring the presence of SARS-CoV-2 infected population. Herein, study aims to apply WBE surveillance and in Bangkok, where highest official covid-19 cases reported Thailand, during fully opening international tourists early 2023. A total 200 wastewater samples (100 influent 100 effluent samples) were collected from 10 treatment plants (WWTPs) January–May RNA was detected by real time qRT-PCR with accounting 51% (102/200). Of these, 88% (88/100) 14% (14/100) samples, respectively. The concentration ranged 4.76 × 102–1.48 105 copies/L. amount increased approximately 4 times lag phase (January–March) log (April–May). Spearman's correlation coefficient revealed that between estimated population weekly statistically significant (p-value = 0.017). had a relationship (r 0.481, p-value < 0.001). Lag analysis warning 1–3 weeks before rising observed. GIS applied spatial-temporal at province level, suggesting dashboard should be further developed.

Язык: Английский

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Variations in the persistence of 5′-end genomic and subgenomic SARS-CoV-2 RNAs in wastewater from aircraft, airports and wastewater treatment plants

Heliyon, Год журнала: 2024, Номер 10(9), С. e29703 - e29703

Опубликована: Апрель 16, 2024

Wastewater sequencing has become a powerful supplement to clinical testing in monitoring SARS-CoV-2 infections the post-COVID-19 pandemic era. While its applications measuring viral burden and main circulating lineages community have proved their efficacy, variations quality coverage across different regions of genome are not well understood. Furthermore, it is unclear how sample origins, extraction concentration methods environmental factors impact reads sequenced from wastewater. Using high-coverage, amplicon-based, paired-end read RNA extracted wastewater collected directly aircraft, pooled aircraft airport buildings or regular plants, we assessed groups with focus on 5'-end region covering leader sequence investigated whether was possible detect subgenomic material recovered We identified distinct patterns persistence genomic types wastewaters existence chimeric mapping non-amplified regions. Our findings suggest that preservation ability reads, though highly susceptible environment processing conditions, may be indicative amount present

Язык: Английский

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Food and Environmental Virology: Use of Passive Sampling to Characterize the Presence of SARS-CoV-2 and Other Viruses in Wastewater

Food and Environmental Virology, Год журнала: 2023, Номер 16(1), С. 25 - 37

Опубликована: Дек. 20, 2023

Fecal shedding of SARS-CoV-2 leads to a renaissance wastewater-based epidemiology (WBE) as additional tool follow epidemiological trends in the catchment treatment plants. As alternative most commonly used composite samples surveillance programs, passive sampling is increasingly studied. However, many sorbent materials different reports hamper comparison results and standardization approach necessary. Here, we compared cost-effective sorption (cheesecloths, gauze swabs, electronegative filters, glass wool, tampons) torpedo-style housings with samples. Despite remarkable variability concentration SARS-CoV-2-specific gene copies, analysis parallel-deposited samplers sewer demonstrated highest rate positive number copies by using cheesecloths. Using this material, monitoring wastewater three small catchments City Dresden resulted 50% (98%). During investigation period, incidence reported cases ranged between 16 170 per 100,000 persons showed no correlation measured concentrations E wastewater. In contrast, constantly higher numbers vs. were found for human adenovirus crAssphage indicating strong differences efficacy methods concerning species investigated. Influenza virus A B sporadically detected allowing results. The study contributes further understanding possibilities limits approaches WBE.

Язык: Английский

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Detection of SARS-CoV-2 in Wastewater Associated with Scientific Stations in Antarctica and Possible Risk for Wildlife

Опубликована: Фев. 5, 2024

Before December 2020, Antarctica had remained free of Covid-19 cases. The main concern during the pandemic was limited health facilities available at Antarctic stations to deal with disease, as well potential impact SARS-CoV-2 on wildlife through reverse zoonosis. In 60 cases emerged in Chilean stations, disrupting summer campaign ongoing isolation needs. RNA detected wastewater several scientific stations. Antarctica, treated is discharged directly into seawater. No studies currently address recovery infectious virus particles from wastewater, but their presence raises risk infecting and initiating new replication cycles. This study highlights initial detection identifying viral via RT-qPCR targeting various genomic regions. virus&#039;s found effluent two plants Maxwell Bay O’Higgins Station King George Island Peninsula, respectively. explores for zoonotic transmission humans due direct release implications such underscore need continued vigilance research.

Язык: Английский

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Wastewater monitoring of SARS‐CoV‐2 gene for COVID‐19 epidemiological surveillance in Tucumán, Argentina

Journal of Basic Microbiology, Год журнала: 2024, Номер 64(8)

Опубликована: Май 7, 2024

Abstract Wastewater‐based epidemiology provides temporal and spatial information about the health status of a population. The objective this study was to analyze report epidemiological dynamics severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) in province Tucumán, Argentina during second third waves disease 2019 (COVID‐19) between April 2021 March 2022. aimed quantify SARS‐CoV‐2 RNA wastewater, correlating it with clinically reported COVID‐19 cases. Wastewater samples ( n = 72) were collected from 16 sampling points located three cities Tucumán (San Miguel de Yerba Buena y Banda del Río Salí). Detection viral nucleocapsid markers (N1 gene) carried out using one‐step reverse transcription‐quantitative polymerase chain reaction (RT‐qPCR). Viral loads determined for each positive sample standard curve. A correlation p < 0.05) observed load (copies/mL) confirmed cases at specific San (SP4, SP7, SP8) both months, May June. Indeed, high concurred peaks This method allowed us follow behavior infection epidemic outbreaks. Thus, wastewater monitoring is valuable indicator that enables anticipation increases tracking progress pandemic. genome‐based surveillance should be implemented as routine practice prepare any future surge infections.

Язык: Английский

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Exposure assessment of severe acute respiratory syndrome coronavirus 2 and norovirus genogroup I/genogroup II in aerosols generated by a municipal wastewater treatment plant

CLEAN - Soil Air Water, Год журнала: 2024, Номер 52(9)

Опубликована: Авг. 15, 2024

Abstract The presence of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) in wastewater and its potential as an airborne transmission source require extensive investigation, particularly treatment plants (WWTPs), where few studies have been conducted. aim this study was to investigate the SARS‐CoV‐2 norovirus (NoV) RNA air samples collected from a municipal WWTP. In addition, assessed risk viral exposure among WWTP employees. both summer winter campaigns study, NoV were quantified wastewater/sludge other than effluent. Viral not detected any collected. assessment with concentrations influent pumping station shows lower calculation historical data provided by AquaVall, but show low‐to‐medium for workers. sensitivity analysis that result model is strongly influenced quantification wastewater. This underscores need investigations into viability wastewater, especially within WWTPs.

Язык: Английский

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