Real-Time On-Site Monitoring of Viruses in Wastewater Using Nanotrap® Particles and RICCA Technologies

Biosensors, Год журнала: 2024, Номер 14(3), С. 115 - 115

Опубликована: Фев. 21, 2024

Wastewater-based epidemiology (WBE) is an effective and efficient tool for the early detection of infectious disease outbreaks in a community. However, currently available methods are laborious, costly, time-consuming due to low concentration viruses presence matrix chemicals wastewater that may interfere with molecular analyses. In present study, we designed highly sensitive “Quick Poop (wastewater fecal waste) Sensor” (termed, QPsor) using joint approach Nanotrap microbiome particles RICCA (RNA Isothermal Co-Assisted Coupled Amplification). Using QPsor, WBE study showed strong correlation standard PEG concentrations qPCR technique. closed format paper-based lateral flow assay, were able demonstrate potential our assay as real-time, point-of-care test by detecting heat-inactivated SARS-CoV-2 virus at 100 copies/mL within one hour. As proof-of-concept demonstration, analyzed viral RNA PMMoV raw samples from treatment plants on-site 60 min. The results show QPsor method can be outbreak combining AI-enabled case model real-time extraction amplification, especially absence intensive clinical laboratory facilities. lab-free, lab-quality capabilities prevalence transmission community contribute management pandemic situations.

Язык: Английский

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Simple SARS-CoV-2 concentration methods for wastewater surveillance in low resource settings

The Science of The Total Environment, Год журнала: 2023, Номер 912, С. 168782 - 168782

Опубликована: Ноя. 23, 2023

Язык: Английский

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Evaluation of colorimetric RT-LAMP for screening of SARS-CoV-2 in untreated wastewater

The Science of The Total Environment, Год журнала: 2023, Номер 907, С. 167964 - 167964

Опубликована: Окт. 20, 2023

This study compared reverse transcription-loop-mediated isothermal amplification (RT-LAMP) and three transcription-quantitative polymerase chain reaction (RT-qPCR) assays targeting the N E genes of SARS-CoV-2 genome for detecting RNA in untreated wastewater samples. RT-qPCR exhibited consistent down to 2 × 102 GC/reaction, with greater analytical sensitivity at 101 GC/reaction by US CDC N1 N2 assays. In contrast, RT-LAMP lower sensitivity, only or above 103 GC/reaction. For seeded samples, assay than N2, E_Sarbeco, Out 30 detected endogenous 29 while identified 27 positive 20 displaying amplifications all technical replicates. Agreement analysis revealed a strong concordance between E_Sarbeco (κ = 0.474) but agreement 0.359). Quantification samples correlation assays, weak correlation. Logistic regression indicated that results correlated quantified 95 % limits detection 3.99 3.47 log10 GC/15 mL, respectively. conclusion, despite may offer advantages surveillance, such as rapid (estimated twice fast), simplicity, making it valuable tool shifting landscape COVID-19 surveillance. Furthermore, LAMP might be prioritized sequencing due reduced sensitivity. These findings support use specific efficient method screening SARS-CoV-2, particularly resource-limited settings.

Язык: Английский

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Strengthening Pathogen and Antimicrobial Resistance Surveillance by Environmental Monitoring in Sub-Saharan Africa: A Stakeholder Survey

Опубликована: Фев. 5, 2024

Background: Waterborne diseases pose a significant global public health threat, compelling enhanced comprehensive surveillance. This study investigates the current infectious disease and antimicrobial resistance (AMR) surveillance systems, including wastewater environmental (WES), in three sub-Saharan African countries: Tanzania, Burkina Faso, Democratic Republic of Congo (DRC). The countries have their specificities regarding networks logistic systems. So, emphasizing need to strengthen existing surveillance, paper advocates for incorporating WES systems specifically designed countries' context monitor waterborne re-emerging pathogens, as well AMR.Methods: National workshops were conducted assess clinical identify priority pathogens new monitoring. Data collected through surveys from experts academia, research, policy, healthcare. Results: Prioritized include (poliovirus, Salmonella Typhi, Vibrio cholerae), respiratory (influenza A&B, SARS-CoV-2), other (Measles Rubella, Mycobacterium tuberculosis). Recommended AMR drug-resistant tuberculosis, spp., methicillin-resistant Staphylococcus aureus, extended-spectrum beta-lactamase carbapenemase-producing E. coli. DRC employ DHSI2-based centralized electronic data collection, complemented by Excel paper-based registries. approach is commonly employed monitoring poliovirus rarely pathogens. Discussion conclusions: valuable tool early detection locally circulating human-derived aiding outbreak detection, data-driven epidemic response, prevention. availability results underscores importance effective sanitation safeguarding human, animal, health. pivotal integrated risk management, preventing outbreaks, protecting drinking water sources, ultimately gaining various UN Sustainable Development Goals. highlights customized line with each country's context, localized approaches AMR.

Язык: Английский

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Evaluation of molecular-based methods for the detection and quantification of Cryptosporidium spp. in wastewater

The Science of The Total Environment, Год журнала: 2024, Номер 947, С. 174219 - 174219

Опубликована: Июнь 24, 2024

Cryptosporidium poses significant public health risks as a cause of waterborne disease worldwide. Clinical surveillance cryptosporidiosis is largely underreported due to the asymptomatic and mildly symptomatic infections, clinical misdiagnoses, barriers access testing. Wastewater overcomes these limitations could serve an effective tool for identifying at population level. Despite its potential, lack standardized wastewater methods spp. challenges implementation design comparability between studies. Thus, this study compared contrasted concentrating oocysts, extracting oocyst DNA, detecting genetic markers. The evaluated concentration included electronegative membrane filtration, Envirocheck HV capsule centrifugation, Nanotrap Microbiome Particles, with without additional immunomagnetic separation purification (except Particles). Oocyst DNA extraction by either DNeasy Powersoil Pro kit QIAamp Mini were impact bead beating freeze-thaw pretreatments on recoveries was assessed. Genetic detection via qPCR assays targeting 18S rRNA gene or wall protein tested. recovery percentages highest centrifugation (39–77 %), followed Particles (24 filtration PBST elution (22 (13 %). Immunomagnetic found be unsuitable interference from matrix. Bead-beating pretreatment enhanced both (314 gc/μL DNA) (238 DNA). In contrast, reduced under 92 likely through degradation. Finally, while specific spp., assay had 5-fold lower limit detect wider range than assay.

Язык: Английский

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Evaluating Nanotrap Microbiome Particles as A Wastewater Viral Concentration Method

Food and Environmental Virology, Год журнала: 2025, Номер 17(1)

Опубликована: Янв. 4, 2025

Abstract Wastewater-based surveillance has emerged as a powerful approach to monitoring infectious diseases within community. Typically, wastewater samples are concentrated before viral analyses improve sensitivity. Current concentration methods vary in time requirements, costs, and efficiency. Here, we evaluated the efficiency bias of novel approach, Nanotrap Microbiome Particles (NMP), wastewater. NMP was target-specific, with significantly lower concentrations bacterial indicator HF183 Carjivirus (formerly crAssphage) relative direct extraction (1.2 × 10 5 vs. 3.4 GC/mL 2.0 1.2 GC/mL, respectively), but higher fecal Pepper Mild Mottle Virus (PMMoV) (1.4 8.4 3 GC/mL). Targeted metagenomic sequencing showed that resulted more unique species reads per sample than extractions ( p < 0.001) by detecting went undetected extractions. Key families identified high abundances were Adenoviridae, Caliciviridae, Herpesviridae, Papillomaviridae, Polyomaviridae. differential ability for concentrating clinically relevant families, suggesting technology should be optimized specific targets implementation.

Язык: Английский

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Wastewater-based epidemiology of influenza viruses: a systematic review

The Science of The Total Environment, Год журнала: 2025, Номер 986, С. 179706 - 179706

Опубликована: Май 30, 2025

Язык: Английский

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A systematic review of influenza virus in water environments across human, poultry, and wild bird habitats

Water Research X, Год журнала: 2023, Номер 22, С. 100210 - 100210

Опубликована: Дек. 13, 2023

Influenza, a highly contagious acute respiratory disease, remains major global health concern. This study aimed to comprehensively assess the prevalence of influenza virus in different aquatic environments. Using 43 articles from four databases, we thoroughly examined water matrices wastewater treatment plants (WTPs) and other human environments, as well poultry habitats areas frequented by migratory wild birds. In WTP influents (10 studies), positivity rates for A ranged 0.0% 97.6%. For B (8 most studies reported no positivity, except three reporting detection 0.8%, 5.6%, 46.9% samples. Within (13 4.2% 76.4%, while environments birds (11 it 0.4% 15.1%. Geographically, were distributed follows: 39.5% Americas, 18.6% Europe, 2.3% South-East Asia Western Pacific. Several subtypes found matrices, including avian (H3N6, H3N8, H4N1, H4N2, H4N6, H4N8, H5N1, H5N8, H6N2, H6N6, H7N9, H0N8, H11N9) seasonal (H1N1 H3N2). The existing literature indicates crucial requirement more extensive future research on this issue. Specifically, emphasizes need method harmonization delves into deserving in-depth such pertaining pig farming low-income countries.

Язык: Английский

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Strengthening Pathogen and Antimicrobial Resistance Surveillance by Environmental Monitoring in Sub-Saharan Africa: A Stakeholder Survey

Опубликована: Фев. 2, 2024

Background: Waterborne diseases pose a significant global public health threat, compelling enhanced comprehensive surveillance. This study investigates the current infectious disease and antimicrobial resistance (AMR) surveillance systems, including wastewater environmental (WES), in three sub-Saharan African countries: Tanzania, Burkina Faso, Democratic Republic of Congo (DRC). The countries have their specificities regarding networks logistic systems. So, emphasizing need to strengthen existing surveillance, paper advocates for incorporating WES systems specifically designed countries' context monitor waterborne re-emerging pathogens, as well AMR.Methods: National workshops were conducted assess clinical identify priority pathogens new monitoring. Data collected through surveys from experts academia, research, policy, healthcare. Results: Prioritized include (poliovirus, Salmonella Typhi, Vibrio cholerae), respiratory (influenza A&amp;B, SARS-CoV-2), other (Measles Rubella, Mycobacterium tuberculosis). Recommended AMR drug-resistant tuberculosis, spp., methicillin-resistant Staphylococcus aureus, extended-spectrum beta-lactamase carbapenemase-producing E. coli. DRC employ DHSI2-based centralized electronic data collection, complemented by Excel paper-based registries. approach is commonly employed monitoring poliovirus rarely pathogens. Discussion conclusions: valuable tool early detection locally circulating human-derived aiding outbreak detection, data-driven epidemic response, prevention. availability results underscores importance effective sanitation safeguarding human, animal, health. pivotal integrated risk management, preventing outbreaks, protecting drinking water sources, ultimately gaining various UN Sustainable Development Goals. highlights customized line with each country's context, localized approaches AMR.

Язык: Английский

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Multisite community-scale monitoring of respiratory and enteric viruses in the effluent of a nursing home and in the inlet of the local wastewater treatment plant

Applied and Environmental Microbiology, Год журнала: 2024, Номер 90(11)

Опубликована: Окт. 10, 2024

ABSTRACT The aim of this study was to evaluate whether community-level monitoring respiratory and enteric viruses in wastewater can provide a comprehensive picture local virus circulation. Wastewater samples were collected weekly at the treatment plant (WWTP) inlet outlet nearby nursing home (NH) Burgundy, France, during winter period 2022/2023. We searched for pepper mild mottle as an indicator fecal content well main [severe acute syndrome coronavirus 2 (SARS-CoV-2), influenza, syncytial virus] (rotavirus, sapovirus, norovirus, astrovirus, adenovirus). Samples analyzed using real-time reverse transcription PCR-based methods. SARS-CoV-2 most frequently detected virus, with 66.7% positive from WWTP 28.6% NH. Peaks consistent chronological incidence infections recorded sentinel surveillance hospital databases. number lower NH than three viruses. Enteric detected, often sapovirus norovirus genogroup II, accounting both 77.8% 57.1% 37%, respectively, large circulation unexpected particular Combined simple optimized methods be valuable tool viral may serve suitable early warning system identifying outbreaks onset epidemics. These results encourage application wastewater-based (WBS) SARS-CoV2, sapovirus. IMPORTANCE WBS provides information on spread epidemic environment appropriate sensitive detection By PCR retirement (connected same collective sewer network), we aimed demonstrate that implementing combined key community sites allows effective occurrence (influenza, SARS-CoV-2) (norovirus, rotavirus, sapovirus) within given population. This analysis localized scale provided new two different sites. Implementing monitor or emergence infectious diseases is important means alerting authorities improving public health management. could participate actively humans, animals, environment.

Язык: Английский

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