Genome-wide diversity and MHC characterisation in a critically endangered freshwater turtle susceptible to disease DOI Creative Commons

Holly V. Nelson,

Luke W. Silver, Toby G. L. Kovacs

и другие.

Immunogenetics, Год журнала: 2025, Номер 77(1)

Опубликована: Май 6, 2025

Abstract Small, isolated populations are often vulnerable to increased inbreeding and genetic drift, both of which elevate the risk extinction. The Bellinger River turtle ( Myuchelys georgesi ) is a critically endangered species endemic single river catchment in New South Wales, Australia. only extant wild population, along with breeding program, face significant threats from viral outbreaks, most notably nidovirus outbreak 2015 that led 90% population decline. To enhance our understanding genomic characteristics species, including genome-wide functional gene diversity, we re-sequenced, assembled, analysed 31 re-sequenced genomes for pure M. N = 31). We manually annotated major histocompatibility complex (MHC), identifying five MHC class I ten II genes investigated diversity across classes . Our results showed low , contexualised through comparison opportunistically sampled backcross animals—offspring F1 hybrids × Emydura macquarii backcrossed 4). However, variation observed within core region extending scaffold 10, exceeded all other macrochromosomes. Additionally, no short-term changes either or immunogenetic were detected following (before; 19, after; 12). Demographic history reconstructions indicated sustained, long-term decline effective size since last interglacial period, accompanied by more recent steep declines. These patterns suggested prolonged isolation reduced have significantly influenced dynamics diversity. It likely contemporary stressors, outbreak, acting on an already genetically depleted population. This study offers new insights into immune annotation data broader implications testudines. findings provide crucial information support future management strategies species.

Язык: Английский

Genome-wide diversity and MHC characterisation in a critically endangered freshwater turtle susceptible to disease DOI Creative Commons

Holly V. Nelson,

Luke W. Silver, Toby G. L. Kovacs

и другие.

Immunogenetics, Год журнала: 2025, Номер 77(1)

Опубликована: Май 6, 2025

Abstract Small, isolated populations are often vulnerable to increased inbreeding and genetic drift, both of which elevate the risk extinction. The Bellinger River turtle ( Myuchelys georgesi ) is a critically endangered species endemic single river catchment in New South Wales, Australia. only extant wild population, along with breeding program, face significant threats from viral outbreaks, most notably nidovirus outbreak 2015 that led 90% population decline. To enhance our understanding genomic characteristics species, including genome-wide functional gene diversity, we re-sequenced, assembled, analysed 31 re-sequenced genomes for pure M. N = 31). We manually annotated major histocompatibility complex (MHC), identifying five MHC class I ten II genes investigated diversity across classes . Our results showed low , contexualised through comparison opportunistically sampled backcross animals—offspring F1 hybrids × Emydura macquarii backcrossed 4). However, variation observed within core region extending scaffold 10, exceeded all other macrochromosomes. Additionally, no short-term changes either or immunogenetic were detected following (before; 19, after; 12). Demographic history reconstructions indicated sustained, long-term decline effective size since last interglacial period, accompanied by more recent steep declines. These patterns suggested prolonged isolation reduced have significantly influenced dynamics diversity. It likely contemporary stressors, outbreak, acting on an already genetically depleted population. This study offers new insights into immune annotation data broader implications testudines. findings provide crucial information support future management strategies species.

Язык: Английский

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