Maximizing Efficiency in SedaDNA Analysis: A Novel Extract Pooling Approach DOI Creative Commons
Victoria Oberreiter, Pere Gelabert,

Florian Brück

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Окт. 20, 2023

Abstract In recent years, the field of ancient DNA (aDNA) has taken a new direction toward studying human population dynamics through sedimentary (sedaDNA), enabling study past ecosystems. However, screening numerous sediment samples from archaeological sites remains time-consuming and costly endeavor, particularly when targeting hominin DNA. Here, we present novel high-throughput method that facilitates fast efficient analysis by applying pooled testing method. Our approach involves combining multiple extracts, allowing users to parallelize laboratory procedures early in sample preparation pipeline while effectively for presence aDNA. Pooled exhibit aDNA signals can then undergo detailed analysis, empty pools are discarded. We have successfully applied our extract pooling various Middle Upper Paleolithic Europe, Asia, Africa. Notably, results reveal an signal discernible even with four negative samples. also demonstrate yield double-stranded libraries increases significantly reducing input, potentially mitigating effects inhibition. By embracing this innovative approach, researchers analyze large numbers preservation, achieving significant cost reductions up 70% hands-on time one-fifth.

Язык: Английский

Physical and biogeochemical responses of Tibetan Plateau lakes to climate change DOI
Liping Zhu, Jianting Ju, Baojin Qiao

и другие.

Nature Reviews Earth & Environment, Год журнала: 2025, Номер unknown

Опубликована: Март 18, 2025

Язык: Английский

Процитировано

2
Lake surface water-heat flux variation and its correlations with meteorological factors on multiscale in the Yamzhog Yumco, south Tibet DOI
Xueqin Zhang, Zheng Jin,

Pengke Shen

и другие.

Journal of Geographical Sciences, Год журнала: 2024, Номер 34(2), С. 397 - 414

Опубликована: Янв. 26, 2024

Язык: Английский

Процитировано

5
Holocene lake response to glacier and catchment changes on the eastern Tibetan Plateau from quantitative conductivity reconstructions based on sedaDNA-derived macrophyte records DOI Creative Commons
Wenjia Li, Xianyong Cao, Kathleen R. Stoof‐Leichsenring

и другие.

Quaternary Science Reviews, Год журнала: 2024, Номер 338, С. 108806 - 108806

Опубликована: Июль 20, 2024

Understanding the response of long-term aquatic environmental changes in lakes to ongoing climate change and human activities is key forecasting future lake conditions. In this study, we infer Holocene limnological Emu Co, a proglacial Nianbaoyuze on eastern Tibetan Plateau, from sedimentary ancient DNA (sedaDNA) data, palynomorph, element, lithological, grain-size analyses. We developed transfer function based Siberia Tibet/China surface applied it Co sedaDNA trace conductivity changes. The results show that was high during 12.6−9.7 cal ka BP, often surpassing 1000 μs cm−1, driven by elevated summer solar radiation. freshwater influx glacial meltwater precipitation, however, reduced lake's as warmed humidified. This led decrease abundance taxa characterised conductivity. Freshwater pulses, triggered climatic fluctuations, likely significant variations within overarching downward trend. By 8 cal. recharge conditions stabilised reached lower level ∼70 cm−1. warm humid mid-Holocene (8−5 BP) provided suitable habitat for many submerged taxa. After 5 growth restricted, indicated shift asexual sexual reproduction macrophytes, suboptimal colder drier climate. Since 1 might have increased nutrient levels, with an enhanced richness macrophytes. Our indicate how millennial-scale hydrological are related retreat catchment alpine region which today facing much greater than global average.

Язык: Английский

Процитировано

3
Eutrophication Conditions in Two High Mountain Lakes: The Influence of Climate Conditions and Environmental Pollution DOI Creative Commons

Fátima Goretti García-Miranda,

Claudia Muro,

Yolanda Alvarado

и другие.

Hydrology, Год журнала: 2025, Номер 12(2), С. 32 - 32

Опубликована: Фев. 13, 2025

The lakes known as El Sol and La Luna are high mountain water deposits located in Mexico within an inactive volcanic system. These of ecological importance because they unique Mexico. However, currently, the have experienced changes their shape increase algae blooms, coupled with degradation basin, which has alerted government entities to need address lakes’ problems. To environmental status Luna, a trophic study was conducted during period 2021–2023, including analysis influence climatic variables, lake quality, eutrophication conditions. state established based on index. Pearson correlations defined interrelation between distinct factors influencing status. registered higher conditions than Luna. identified seasonal eutrophic transitioning from oligotrophic mesotrophic, showing levels chlorophyll, total phosphorus, nitrogen low transparency. principal altering were pollution variables (precipitation ambient temperature). Eutrophication prime factor impacting perimeter loss at Sol, whereas it due decline precipitation.

Язык: Английский

Процитировано

0
Annual 30 m land cover dataset on the Tibetan Plateau from 1990 to 2023 DOI Creative Commons

Siya Li,

Quansheng Ge, Fubao Sun

и другие.

Scientific Data, Год журнала: 2025, Номер 12(1)

Опубликована: Март 27, 2025

Accurate land cover data was fundamental for formulating sound planning and sustainable development strategies. This study focused on the Tibetan Plateau (TP), a globally sensitive ecological area, developed locally tailored annual 30 m resolution dataset from 1990 to 2023 (TPLCD). Leveraging Google Earth Engine (GEE) platform Landsat processing, LandTrendr employed generate robust, high-precision training samples. Subsequently, random forest classification spatiotemporal smoothing strategies were applied precisely map dynamics of TP. Rigorous validation through visual interpretation, authoritative third-party datasets (Geo-Wiki GLCVSS), thematic cross-comparisons, revealed an overall accuracy 84.8%, Kappa coefficient 0.78, fully affirming dataset's high reliability. provided invaluable empirical evidence understanding vulnerability adaptability TP's ecosystem.

Язык: Английский

Процитировано

0
Socioeconomic status, lake knowledge, and community participation in the sustainable Lake Limboto management, Gorontalo Regency DOI Creative Commons
Ramla Hartini Melo, Moch Rio Pambudi, Alim S Niode

и другие.

Journal of Water and Land Development, Год журнала: 2024, Номер unknown, С. 177 - 182

Опубликована: Март 27, 2024

Lake Limboto, situated in Gorontalo, Indonesia, confronts severe threats jeopardizing its sustainability. Widespread deforestation the watershed area has led to excessive sedimentation within lake, consequently diminishing water storage capacity and compromising flood control function. This degradation manifested itself downstream droughts during dry season flooding rainy season. Historical data reveals a stark reduction Limboto’s size, plummeting from approximately 3,644.5 ha 1991 around 2,693.9 2017. study aims provide comprehensive examination of interplay between socioeconomic status local community their understanding lake ecosystem. Furthermore, it delves into how these factors produce synergies that shape impact involvement sustainable management initiatives. Hypothesis testing yielded significant results, affirming existence positive correlation status, knowledge ecosystem, active participation efforts. The findings underscore critical importance need be considered when designing strategies for preservation Limboto. Integrating conservation initiatives is necessary, given intrinsic relationship with lake. By acknowledging leveraging nexus ecological knowledge, participation, stakeholders can formulate more effective inclusive safeguarding integrity. contributes valuable insights policymakers, environmentalists, communities alike, emphasizing necessity collaborative efforts ensure long-term resilience vitality

Язык: Английский

Процитировано

2
SedaDNA reveals mid-to late Holocene aquatic plant and algae changes in Luanhaizi Lake on the Tibetan Plateau DOI
Hanqiu Xu,

Lian‐Fang Feng,

Naimeng Zhang

и другие.

Palaeogeography Palaeoclimatology Palaeoecology, Год журнала: 2024, Номер 650, С. 112344 - 112344

Опубликована: Июнь 22, 2024

Язык: Английский

Процитировано

2
Impacts of human and herbivorous feces on lake surface sediments of the Tibetan Plateau based on fecal stanol proxies DOI Creative Commons
Cunlin Li, Liping Zhu, Jianting Ju

и другие.

Ecological Indicators, Год журнала: 2023, Номер 158, С. 111487 - 111487

Опубликована: Дек. 26, 2023

Lake ecosystems on the Tibetan Plateau (TP) are highly susceptible to external input owing their fragile environment. However, less study has been done focus direct influences of anthropogenic activities lake sediments by human activities. In this study, we performed first large-scale investigation use fecal stanol proxies in surface assess impact and herbivorous feces TP lakes. The 75 investigated lakes were divided into three types (I, II, III) according cluster analysis ratios R1′ R2′. Fecal stanols type I originate from a mixture feces; II primarily with minimal feces. contrast, III mainly derived (coprostanol concentration > 100 ng/g), mixed source cholesterol reduction 10–100 situ < 10 ng/g). Among investigated, 9 % had significant contamination, 48 moderate or slight 43 uncontaminated. Domestic animal main moderately slightly contaminated central TP. There contamination 52 did not definitely show presence matter. This important implications for assessment provides reference early change paleo-human activity research.

Язык: Английский

Процитировано

2
Impacts of Environmental Factors on Over-Wintering Aquatic Bird Communities in Yamzho Yumco Lake, China DOI Open Access
Lei Xu, Le Yang, Lü Cai

и другие.

Sustainability, Год журнала: 2023, Номер 16(1), С. 254 - 254

Опубликована: Дек. 27, 2023

Global climate change has significantly impacted waterbird communities in the warming and humidifying wetlands of Qinghai–Tibet Plateau. However, further research is needed as these transition to warmer drier states. This study focused on diversity richness wintering birds alpine Yamzho Yumco Lake. By analyzing data collected from 17 sampling sites over seven years, we examined abundance changes waterbirds lake surrounding land determined relationship between structure data. The results have shown that abundance, richness, α-diversity index fluctuated. Wintering were positively correlated with monthly mean temperature higher negatively total precipitation water level. Principal component analysis (PCA) showed contributed most waterbirds. Bird drying Lake same results. As temperatures continue increase decreases, number may also increase. We highlighted indicative role responses humidification aridification suggest decision makers strengthen monitoring Reserve, restrict human activities, improve resource management, actively respond challenges brought about by change.

Язык: Английский

Процитировано

1
Maximizing efficiency in sedimentary ancient DNA analysis: a novel extract pooling approach DOI Creative Commons
Victoria Oberreiter, Pere Gelabert,

Florian Brück

и другие.

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Авг. 20, 2024

In the last few decades, field of ancient DNA has taken a new direction towards using sedimentary (sedaDNA) for studying human and mammalian population dynamics as well past ecosystems. However, screening numerous sediment samples from archaeological sites remains time-consuming costly endeavor, particularly when targeting hominin DNA. Here, we present novel high-throughput method that facilitates fast efficient analysis by applying pooled testing approach. This combines multiple extracts, enabling early parallelization laboratory procedures effective aDNA screening. Pooled with detectable signals undergo detailed analysis, while empty pools are discarded. We have successfully applied our to Middle Upper Paleolithic in Europe, Asia, Africa. Notably, results reveal an signal discernible even four negative samples. also demonstrate yield double-stranded libraries increases significantly reducing extract input, potentially mitigating effects inhibition. By embracing this innovative approach, researchers can analyze large numbers preservation, achieving significant cost reductions up 70% hands-on time one-fifth.

Язык: Английский

Процитировано

0