Impact of Brake Wear Particles on Eukaryotic Cell Viability and Associated Oxidative Stress Responses DOI Creative Commons

Lina Trečiokaitė,

Yurii Tsybrii, Oleksii Nosko

и другие.

Lubricants, Год журнала: 2024, Номер 12(12), С. 449 - 449

Опубликована: Дек. 16, 2024

In this study, the cytotoxic effects of brake wear particles (≥250 nm ceramic/ceramic (CCWPs) and ≤100 ceramic/steel (CSWPs)) 100 iron (III) oxide ultrafine (IOUFPs) on human lung carcinoma (A549) Chinese hamster ovary (CHO) cells were investigated. Cell viability was determined using MTT Calcein AM methods. Oxidative stress assessed by measuring reactive oxygen species (ROS), intracellular reduced glutathione (GSH), malondialdehyde (MDA) concentrations under exposure to above in concentration range 10–80 µg/mL. The initial assessments CCWPs CSWPs cell performed after a 4-h but later extended 24 h investigate time-dependent oxidative stress. assays indicated that A549 are less susceptible than CHO when exposed for both 4 h. This study highlights induced CCWPs, CSWPs, IOUFPs is cell-specific. While did not affect (GSH) levels cells, it significantly GSH with exception 80 Both increased lipid peroxidation types; however, demonstrated lower sensitivity these treatments.

Язык: Английский

Impact of Brake Wear Particles on Eukaryotic Cell Viability and Associated Oxidative Stress Responses DOI Creative Commons

Lina Trečiokaitė,

Yurii Tsybrii, Oleksii Nosko

и другие.

Lubricants, Год журнала: 2024, Номер 12(12), С. 449 - 449

Опубликована: Дек. 16, 2024

In this study, the cytotoxic effects of brake wear particles (≥250 nm ceramic/ceramic (CCWPs) and ≤100 ceramic/steel (CSWPs)) 100 iron (III) oxide ultrafine (IOUFPs) on human lung carcinoma (A549) Chinese hamster ovary (CHO) cells were investigated. Cell viability was determined using MTT Calcein AM methods. Oxidative stress assessed by measuring reactive oxygen species (ROS), intracellular reduced glutathione (GSH), malondialdehyde (MDA) concentrations under exposure to above in concentration range 10–80 µg/mL. The initial assessments CCWPs CSWPs cell performed after a 4-h but later extended 24 h investigate time-dependent oxidative stress. assays indicated that A549 are less susceptible than CHO when exposed for both 4 h. This study highlights induced CCWPs, CSWPs, IOUFPs is cell-specific. While did not affect (GSH) levels cells, it significantly GSH with exception 80 Both increased lipid peroxidation types; however, demonstrated lower sensitivity these treatments.

Язык: Английский

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