Water,
Год журнала:
2024,
Номер
16(24), С. 3571 - 3571
Опубликована: Дек. 12, 2024
Antibiotic
resistance
is
a
silent
global
crisis
intensified
by
the
recent
pandemic
of
coronavirus
disease
2019
(COVID-19).
To
address
this
growing
threat,
wastewater-based
surveillance
(WBS)
emerging
as
promising
public
health
tool
for
monitoring
antibiotic
within
communities.
Our
meta-analysis
aims
to
reveal
landscape
antibiotic-resistance
genes
(ARGs)
in
wastewater
during
and
after
COVID-19
pandemic.
The
analysis
included
samples
collected
between
2020
2024
from
five
countries
across
three
continents:
Asia
(China),
Europe
(United
Kingdom
Russia),
North
America
States
Canada).
findings
showed
higher
observed
ARGs
Russia
China
despite
their
small
sample
size,
while
USA
more
diverse
ARGs.
Distinct
patterns
were
European
American
(p-value
<
0.001).
We
identified
2483
ARGs,
with
multidrug-resistant
(MDR)
dominating
most
regions
accounting
almost
45%
all
detected
Europe.
Country-specific
indicator
22
unique
Russia,
3
each
UK
Canada,
2
specific
China.
Continentally,
100
Asia,
38
Europe,
18
America.
These
highlight
regional
variations
ARG
profiles,
emphasizing
urgent
need
region-specific
strategies
combat
threat.
Additionally,
our
study
further
supports
value
WBS
valuable
resistance.
PLoS ONE,
Год журнала:
2025,
Номер
20(5), С. e0324083 - e0324083
Опубликована: Май 15, 2025
Introduction
Antimicrobial
resistance
(AMR)
has
become
one
of
the
leading
global
health
threats.
It
is
critical
to
understand
burden
AMR,
particularly
among
vulnerable
populations
such
as
people
with
intellectual
disabilities
residing
in
long-term
care
facilities
(ID-LTCFs).
Traditional
study
methods
estimate
AMR
these
settings,
rectal
swabs
measure
prevalence
MDRO
carriage,
are
considered
burdensome
for
this
population.
This
underscores
importance
a
non-invasive
method
assess
living
ID-LTCFs.
publication
describes
protocol
an
alternative
approach
specifically
Extended
Spectrum
Beta-Lactamase
(ESBL)
and
carbapenemase-producing
Enterobacterales
(CPE),
ID-LTCFs
Netherlands,
through
wastewater
measurements
combined
analysis
stool
collected
from
diaper
material.
The
provides
detailed
information
about
design
methodologies
proposed
pilot
study.
Methods
Wastewater
samples
will
be
obtained
sewers
ID-LCTFs
using
passive
samplers.
Additionally,
considerable
part
ID-LTCF
residents
incontinent,
material
which
incontinent
participating
cultured
on
selective
media
detect
ESBL-producing
carbapenem
producing
(CPE)
strains.
Determination
strains
carried
out
MALDI-TOF
phenotypical
tests
confirm
ESBL
CPE
In
samples,
bacterial
concentrations
determined,
expressed
colony
forming
unit
(CFU)
per
sampler,
while
presence
or
absence
reported
proportions.
Ethics
dissemination
procedures
described
conducted
line
principles
outlined
Declaration
Helsinki,
Code
Conduct
Health
research,
well
General
Data
Protection
Regulation.
Approval
advance
by
ethical
research
committee
institutional
review
board
deemed
unnecessary
current
national
European
legislation.
Heliyon,
Год журнала:
2024,
Номер
10(10), С. e30720 - e30720
Опубликована: Май 1, 2024
Wastewater-based
epidemiology
(WBE)
is
a
robust
tool
for
disease
surveillance
and
monitoring
of
pharmaceutical
consumption.
However,
tuberculosis
(TB)
drug
consumption
faces
challenges
due
to
limited
data
availability.
This
study
aimed
optimise
methods
detecting
TB
drugs
in
treated
untreated
wastewater
from
four
African
countries:
South
Africa,
Nigeria,
Kenya,
Cameroon.
The
limit
detection
(LOD)
these
ranged
minimum
2.20
(±1.02)
rifampicin
maximum
2.95
(±0.79)
pyrazinamide.
A
parallel
trend
was
observed
concerning
the
quantification
(LOQ),
with
reporting
lowest
average
LOQ
7.33
(±3.44)
pyrazinamide
showing
highest
9.81
(±2.64).
variance
LOD
values
could
be
attributed
factors
such
as
polarity.
Erythromycin
exhibited
moderately
polar
LogP
(2.6
2.95),
indicating
higher
lipid
affinity
lower
water
affinity.
Conversely,
ethambutol,
pyrazinamide,
isoniazid
displayed
(-0.059,
-0.6,
-0.7),
suggesting
greater
revealed
that
storing
samples
up
5
days
did
not
result
significant
concentration
loss,
reduction
remaining
below
1
log
throughout
storage
period.
Application
optimised
method
both
unveiled
varied
results.
Detection
frequencies
among
drugs,
ethambutol
consistently
most
detected,
while
were
least
detected
only
two
countries.
Most
had
undetectable
concentrations,
ranging
1.21
ng/mL
erythromycin
54.61
isoniazid.
variability
may
suggest
differences
within
connected
communities.
In
samples,
detectable
concentrations
1.27
10.20
ethambutol.
Wastewater
treatment
plants
variable
removal
efficiencies
different
emphasising
need
further
optimisation.
Detecting
suggests
potential
surface
contamination
subsequent
risks
human
exposure,
underscoring
continued
research's
importance.
Biosafety and Health,
Год журнала:
2023,
Номер
6(1), С. 50 - 58
Опубликована: Дек. 29, 2023
Worldwide,
infections
caused
by
drug-resistant
pathogens
constitute
a
significant
challenge
threatening
therapeutic
efforts.
According
to
the
World
Health
Organization
(WHO),
antimicrobial
resistance
(AMR)
ranks
among
top
10
global
public
health
threats.
Organisms
with
high
rate
of
multiple
host
adaptivity,
genetic
diversity
(multiple
lineages),
virulence
factors,
and
exchange
have
been
isolated
from
various
sources
(humans,
animals,
environment)
even
without
exposure
prior
antibiotics.
Till
now,
source
AMR
how
resistant
clones
are
selected
in
environment
remain
largely
elusive,
potential
anthropogenic
transmission
has
reported
different
studies.
Various
pathogens,
lineages,
clones,
outbreak
clusters,
plasmid
replicates,
genes
that
play
critical
role
dissemination
identified.
Maintenance
certain
multidrug-resistant
(MDR)
determinants
also
shown
enhance
or
support
propagation
MDR.
So
far,
advances
made
understanding
burden
AMR.
However,
overcoming
requires
holistic
approach,
as
there
is
no
single
approach
sufficient
precision
curb
threat.
While
strengthening
surveillance
efforts
essential,
we
shown,
need
intensify
strengthen
interventions,
especially
priority
regions
such
Africa.
Herein,
discussed
humans,
(non-medical
drivers).
We
further
delved
into
big
questions
on
Africa
interventions
involving
vaccines
other
viable
biomaterials
could
be
pivotal
reducing
barest
minimum.
Heliyon,
Год журнала:
2024,
Номер
10(9), С. e29601 - e29601
Опубликована: Апрель 30, 2024
Antibiotic
resistance
(AR)
is
a
major
global
health
concern,
but
current
surveillance
efforts
primarily
focus
on
healthcare
settings,
leaving
lack
of
understanding
about
AR
across
all
sectors
the
One
Health
approach.
To
bridge
this
gap,
wastewater
provides
cost-effective
and
efficient
method
for
monitoring
within
population.
In
study,
we
implemented
program
by
effluent
from
two
large-scale
municipal
treatment
plants
situated
in
Isfahan,
central
region
Iran.
These
covered
distinct
catchment
regions
served
combined
population
million
residents.
Furthermore,
effect
physicochemical
microbial
characteristics
including
biological
oxygen
demand
(BOD),
chemical
(COD),
total
suspended
solids
(TSS),
temperature,
coliforms
Journal of Microbiological Methods,
Год журнала:
2024,
Номер
228, С. 107069 - 107069
Опубликована: Ноя. 17, 2024
Methods
that
are
used
to
characterise
microbiomes
and
antimicrobial
resistance
genes
(ARGs)
in
wastewater
not
standardised.
We
shotgun
metagenomic
sequencing
(SM-Seq),
RNA
(RNA-seq)
targeted
qPCR
compare
microbial
ARG
diversity
the
influent
a
municipal
treatment
plant
Australia.
ARGs
were
annotated
with
CARD-RGI
MEGARes
databases,
bacterial
was
characterised
by
16S
rRNA
gene
SM-Seq,
species
annotation
SILVA/GreenGenes
databases
or
Kraken2
NCBI
nucleotide
database
respectively.
CARD
identified
evenly
distributed
profiles
but
detected
richer
array
of
(richness
=
475
vs
320).
Qualitatively,
encoding
for
aminoglycoside,
macrolide-lincosamide-streptogramin
multidrug
most
abundant
all
examined
databases.
RNA-seq
only
32
%
there
concordance
qualitative
identification
macrolide-lincosamide,
phenicol,
sulfonamide
SM-Seq
RNA-seq.
confirmed
detection
some
ARGs,
including
OXA,
VEB
EREB,
influent.
For
bacteria,
equally
effective
population
profiling
at
phyla
class
level.
However,
significantly
higher
richness
15,000
3750).
These
results
demonstrate
sufficient
surveillance
wastewater.
more
precise
quantification
however,
presented
better
resolution.
The
functionality
confirmed,
general
agreement
on
putative
phenotypic
profile
observed
between
RNA-Seq
SM-Seq.
Infection and Drug Resistance,
Год журнала:
2024,
Номер
Volume 17, С. 5117 - 5128
Опубликована: Ноя. 1, 2024
is
an
emerging
human
pathogen
that
causes
nosocomial
infections.
Its
prevalence
and
spread
in
the
environment
should
not
be
ignored.
This
study
identified
characterized
Water,
Год журнала:
2024,
Номер
16(24), С. 3571 - 3571
Опубликована: Дек. 12, 2024
Antibiotic
resistance
is
a
silent
global
crisis
intensified
by
the
recent
pandemic
of
coronavirus
disease
2019
(COVID-19).
To
address
this
growing
threat,
wastewater-based
surveillance
(WBS)
emerging
as
promising
public
health
tool
for
monitoring
antibiotic
within
communities.
Our
meta-analysis
aims
to
reveal
landscape
antibiotic-resistance
genes
(ARGs)
in
wastewater
during
and
after
COVID-19
pandemic.
The
analysis
included
samples
collected
between
2020
2024
from
five
countries
across
three
continents:
Asia
(China),
Europe
(United
Kingdom
Russia),
North
America
States
Canada).
findings
showed
higher
observed
ARGs
Russia
China
despite
their
small
sample
size,
while
USA
more
diverse
ARGs.
Distinct
patterns
were
European
American
(p-value
<
0.001).
We
identified
2483
ARGs,
with
multidrug-resistant
(MDR)
dominating
most
regions
accounting
almost
45%
all
detected
Europe.
Country-specific
indicator
22
unique
Russia,
3
each
UK
Canada,
2
specific
China.
Continentally,
100
Asia,
38
Europe,
18
America.
These
highlight
regional
variations
ARG
profiles,
emphasizing
urgent
need
region-specific
strategies
combat
threat.
Additionally,
our
study
further
supports
value
WBS
valuable
resistance.
