Proteomic examination of polyester‐polyurethane degradation by Streptomyces sp. PU10: Diverting polyurethane intermediates to secondary metabolite production

Microbial Biotechnology, Год журнала: 2024, Номер 17(3)

Опубликована: Март 1, 2024

Abstract Global plastic waste accumulation has become omnipresent in public discourse and the focus of scientific research. Ranking as sixth most produced polymer globally, polyurethanes (PU) significantly contribute to environmental pollution due toxicity their building blocks, such diisocyanates. In this study, effects PU on soil microbial communities over 18 months were monitored revealing that it had marginal diversity. However, Streptomyces sp. PU10, isolated from PU‐contaminated soil, proved exceptional degradation a soluble polyester‐PU (Impranil) across range temperatures with 96% 10 g/L 48 h. Proteins involved metabolic changes occurring strain Impranil sole carbon source further investigated employing quantitative proteomics. The proposed mechanism implicated action three enzymes: polyester‐degrading esterase, urethane bond‐degrading amidase an oxidoreductase. Furthermore, proteome data revealed intermediates incorporated into PU10 metabolism via fatty acid pathway subsequently channelled polyketide biosynthesis. Most notably, production tri‐pyrrole undecylprodigiosin was confirmed paving way for establishing upcycling strategies bioactive metabolites using strains.

Язык: Английский

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Study on biodegradation of polyurethane coating on PCB by Aspergillus brasiliensis in space

Chemical Engineering Journal, Год журнала: 2024, Номер 490, С. 151514 - 151514

Опубликована: Апрель 29, 2024

Язык: Английский

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In Situ Preparation of Composite Scaffolds Based on Polyurethane and Hydroxyapatite Particles for Bone Tissue Engineering

ACS Omega, Год журнала: 2025, Номер 10(6), С. 5478 - 5488

Опубликована: Фев. 3, 2025

This article details the in situ preparation of composite scaffolds using polyurethane (PU) and HAp (hydroxyapatite), focusing on unique properties buriti oil (Mauritia flexuosa L.) applicable to tissue engineering. PU derived from vegetable oils, particularly oil, has shown promise bone repair due its rich bioactive compounds. Buriti is an excellent candidate for manufacturing these materials as it compounds such carotenoids, tocopherols, fatty acids, which have antioxidant anti-inflammatory properties. Furthermore, oleic acid principal acid, been investigated dispersant. research aimed synthesize a polyol incorporate different concentrations into polymeric matrix through polymerization. The chemical composition obtained, distribution hydroxyapatite particles matrix, thermal stability were evaluated Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive analysis (EDS), thermogravimetry (TGA). In addition, investigate biocompatibility, MTT tests (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium) conducted rat bone-marrow-derived mesenchymal stem cells (BMMSC). Characterizations confirm formation presence did not show cytotoxicity.

Язык: Английский

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Biodegradation of commercial polyester polyurethane by a soil-borne bacterium Bacillus velezensis MB01B: Efficiency, degradation pathway, and in-situ remediation in landfill soil

Environmental Pollution, Год журнала: 2024, Номер 363, С. 125300 - 125300

Опубликована: Ноя. 12, 2024

Язык: Английский

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