bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Авг. 25, 2024
Abstract
Background
Pre-mRNA
alternative
splicing
(AS)
plays
essential
roles
in
response
to
environmental
stimuli
plants.
However,
the
universal
and
specific
features
of
diverse
conditions
remain
not
fully
understood.
Recent
studies
have
shown
co-transcriptional
characteristics
splicing,
which
lead
reasonable
speculation
that
elements
or
factors
regulating
transcription
can
also
affect
splicing.
Among
which,
effects
on
plants
under
are
still
confusing.
A
large
amount
0f
public
available
RNA
sequencing
data
valuable
resources
be
re-analyzed
for
answering
questions
beyond
aims
their
original
studies.
Results
We
explored
AS
using
a
standard
RNA-seq
dataset
TENOR,
stems
from
rice
samples
controlled
provide
comprehensive
comparable
analysis
various
conditions.
found
widely
occurs
stimuli,
with
significant
tissue
specificity,
temporal
dynamics,
commonality
among
different
stresses
treatments
as
well
difference
between
differential
expressed
genes
(DASGs
DEGs)
stimuli.
The
majority
DASGs
factors.
correlation
shows
expression
level
is
significantly
correlated
PSI
events.
predominant
correlating
events
come
bHLH,
bzip
hsfa
families.
validated
by
analyzing
factor
mutants
substantial
wild
type.
Furthermore,
was
discovered
levels
Conclusion
SFs
TFs
environments.
propose
might
regulated
download
partly
changing
patterns
own
further
regulate
SFs.
This
work
illuminate
possible
mechanisms
modulate
plant,
especially
Land Degradation and Development,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 6, 2025
ABSTRACT
Cadmium
(Cd)‐contaminated
rice
paddies
have
been
a
primary
environmental
concern
worldwide.
Biochar
or
modified
biochar
application
favors
reducing
Cd
availability
in
paddy
soil,
which
has
demonstrated
physical
and
chemical
terms.
However,
we
know
little
about
soil
microbial
responses
after
application.
In
this
study,
revealed
the
immobilization
effect
mechanism
of
fields
by
novel
phosphorite
magnetic
(PMCB)
from
perspective
response.
The
results
suggested
that
PMCB
facilitated
conversion
highly
active
to
less
reduced
uptake
improving
physicochemical
properties
functional
bacterial
gene
abundance.
Compared
with
control,
acetic
acid
extractable
reducible
contents
content
brown
decreased
7.4%–18.4%,
16.2%–19.9%,
44.0%–47.9%,
respectively,
residual
increased
16.3%–25.8%
under
treatment.
A
decrease
sqr
fccB
abundances
(downregulated
6.3%
12.4%,
respectively)
inhibited
CdS
oxidative
dissolution
0.2%
Conversely,
other
treatments
stimulated
abundant
proliferation
microbes
(e.g.,
Anaeromyxobacter
,
Geobacter
Thiobacillus
)
these
genes
while
suppressing
sulfide‐producing
abundance
(sreA
phsA
30.5%–73.4%
3.3%–11.3%,
respectively).
This
result
implies
Fe(III)
mineral
reduction
oxidation
lead
limited
secondary
release
Cd.
Additionally,
aqueous‐phase
analysis
immobilized
mainly
coprecipitation
PO
4
3−
CO
3
2−
(34.5%)
ion
exchange
(32.2%),
followed
surface
complexation
(18.7%)
cation
π‐bonding
(13.1%).
Water,
Год журнала:
2024,
Номер
16(24), С. 3719 - 3719
Опубликована: Дек. 23, 2024
The
contamination
of
heavy
metals
(HMs)
in
the
topsoil
agricultural
and
pastoral
areas
threatens
yield
quality
agriculture
animal
husbandry
also
endangers
regional
ecological
security
human
health.
This
study
analyzed
HMs
(Cd,
Pb,
As,
Cu,
Zn,
Hg,
Mn)
with
intensive
activities
to
identify
their
accumulation,
source
apportionment,
ecological-health
risks
using
geochemical
methods
coupled
positive
matrix
factorization
(PMF).
It
was
uncovered
that
concentrations
presented
varying
scales
enrichment
levels
topsoil.
However,
except
for
only
one
sample,
residual
fell
within
associated
risk
screening
values
(the
RSVs).
combined
assessment
geo-accumulation
index
(Igeo)
factor
(EF)
pointed
Hg
Cd
were
primary
contaminants.
PMF
identified
four
sources
including
natural
geogenic,
industry
mining,
composite
(caused
by
multi-anthropogenic
activities),
accounting
33.7%,
41.0%,
7.7%,
17.6%
contribution
rates,
respectively.
potential
largely
attributed
accumulation
followed
Cd.
non-carcinogenic
both
groups
beneath
acceptable
threshold,
prospective
carcinogenic
health
threats
posed
Cd,
Pb
different
should
not
be
ignored,
particularly
concerning
children.
is
great
significance
control
pollution
restore
soil
cleanliness
area.
