A systematic discussion and comparison of the construction methods of synthetic microbial community

Synthetic and Systems Biotechnology, Год журнала: 2024, Номер 9(4), С. 775 - 783

Опубликована: Июнь 20, 2024

Synthetic microbial community has widely concerned in the fields of agriculture, food and environment over past few years. However, there is little consensus on method to synthetic from construction functional verification. Here, we review concept, characteristics, history applications community, summarizing several methods for construction, such as isolation culture, core microbiome mining, automated design, gene editing. In addition, also systematically summarized design concepts, technological thresholds, applicable scenarios various methods, highlighted their advantages limitations. Ultimately, this provides four efficient, detailed, easy-to-understand -follow steps with major implications agricultural practices, production, environmental governance.

Язык: Английский

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A novel fungal and bacterial consortium promotes the degradation of rice straw: Conditions optimization and degradation properties

International Biodeterioration & Biodegradation, Год журнала: 2024, Номер 194, С. 105875 - 105875

Опубликована: Июль 28, 2024

Язык: Английский

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Physical–Chemical–Biological Pretreatment for Biomass Degradation and Industrial Applications: A Review

Waste, Год журнала: 2024, Номер 2(4), С. 451 - 473

Опубликована: Ноя. 4, 2024

Lignocellulosic biomass, including agricultural, forestry, and energy crop waste, is one of Earth’s most abundant renewable resources, accounting for approximately 50% global resources. It contains cellulose, hemicellulose, lignin, making it crucial biofuels bio-based chemicals. Due to its complex structure, single-pretreatment methods are inefficient, leading the development combined pretreatment technologies. These enhance cellulose accessibility conversion efficiency. This paper analyzes principles, advantages, disadvantages various their practical benefits. highlights recent research achievements applications in biofuel, biochemical production, feed. By integrating multiple methods, biomass degradation efficiency can be significantly improved, consumption reduced, chemical reagent use minimized. Future advancements physical, chemical, biological technologies will further utilization efficiency, reduce consumption, protect environment, providing robust support sustainable ecological protection.

Язык: Английский

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Effect of Corn Straw Treated with Lactobacillus plantarum and Cellulase on Ruminal Fermentation and Microbiota of Hu Sheep

Fermentation, Год журнала: 2024, Номер 10(8), С. 402 - 402

Опубликована: Авг. 2, 2024

This study aimed to assess how the bacterial–enzyme co-fermentation of corn straw affects fermentation quality, digestion rate in Hu sheep, and rumen microorganisms. Orthogonal experiments were utilized establish optimal conditions, which subsequently applied trials involving four groups: group A (control), B (enzyme added), C (bacteria D + enzyme). The results show that conditions addition 2% flour, cottonseed meal, 60% moisture. In comparison with A, demonstrated most favorable outcomes, notably reducing pH cellulose content while enhancing lactic acid content. relative abundances Pantoea Weissella reduced, whereas those Lactiplantibacillus Limosilactobacillus increased, fermented straw. animal experiments, exhibited significantly higher digestibility NDF ADF; total VFA, acetic acid, NH3-N contents; enzyme activity compared groups B. Additionally, Prevotella, NK4A214-group, Entodinium, Polyplastron Dasytricha Enoploplastron decreased, A. It can be concluded Lactobacillus plantarum cellulase treatments enhance nutritional value by improving ruminal regulating sheep microbiota.

Язык: Английский

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Microbial Dynamics, Metabolite Profiles, and Chemical Composition in Saccharomyces cerevisiae and Kluyveromyces marxianus Co-Culture During Solid-State Fermentation

Food Bioscience, Год журнала: 2025, Номер unknown, С. 105849 - 105849

Опубликована: Янв. 1, 2025

Язык: Английский

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Solid-state fermentation of rapeseed meal using Schizochytrium ATCC 20888 to improve docosahexaenoic acid and degradate toxin

Food Bioscience, Год журнала: 2025, Номер unknown, С. 105946 - 105946

Опубликована: Янв. 1, 2025

Язык: Английский

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Fermented Feed Promotes Gut Development by Enhancing Intestinal Stem Cell Expansion via Activation of the Wnt/β-Catenin Signaling Pathway

Fermentation, Год журнала: 2025, Номер 11(2), С. 52 - 52

Опубликована: Янв. 23, 2025

Fermented feed is extensively used in animal production due to their improved palatability and efficient utilization. This study aimed explore the effects of fermented on growth performance gut health, particularly through modulation intestinal stem cells (ISCs). Twenty-four 28-day-old male weaned piglets were randomly assigned into two groups (n = 12): a control group fed basal diet, an experimental diet replaced with 6% mixed (FMF) for 28 days. The results indicated that FMF significantly elevated rate, intake, nutrient digestibility (p < 0.05). Furthermore, supplementation increased jejunal villus height, transepithelial electrical resistance (TEER) values, expression tight junction proteins (ZO-1 Claudin1) Immunohistochemistry (IHC) analysis revealed number Olfm4+ ISCs PCNA+ mitotic crypts, facilitating differentiation enterocytes (Villin), goblet (MUC2), enteroendocrine (CHGA). Conversely, protein Cleaved Caspase-3 was decreased Notably, Wnt/β-catenin signaling pathway, including FZD7 Active β-catenin, upregulated jejunum after supplementation. Consistent these findings, organoids derived from crypts demonstrated enhanced Lgr5, PCNA, KRT20, β-catenin These suggest promotes development by enhancing ISC proliferation via activation pathway.

Язык: Английский

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Probiotic–Enzyme Synergy Regulates Fermentation of Distiller’s Grains by Modifying Microbiome Structures and Symbiotic Relationships

Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер 73(9), С. 5363 - 5375

Опубликована: Фев. 20, 2025

The high fiber content and low rumen digestibility prevent the efficient use of distiller's grains (DGS) in ruminant feeds. This study investigated effects probiotics (Lactiplantibacillus plantarum Bacillus subtilis) enzymes (β-glucanase, xylanase, β-mannanase, cellulase) on DGS nutrient content, ruminal degradability, microbial communities under anaerobic storage for 30 days. Groups included control (C), (B), (E), their mixture (EB). As compared to groups C, B, E, neutral detergent (NDF), acid (ADF), hemicellulose, cellulose contents were significantly decreased degradability NDF ADF at 48 h was increased group EB (p < 0.05). Enzyme activities affected bacterial abundance, these negatively correlated with fibrous components. abundances Rummeliibacillus but positively enzymes. symbiotic relationship between Anaerocolumna sustained synergistic Co-fermentation enzyme additives enhanced nutritional value DGS, which associated not only probiotic-enzyme synergy also variations dominant microbes microbiome commensal relationships.

Язык: Английский

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Lignin-based biochar with improved properties derived from the microbial combined chemical pretreatment of corn straw for efficient antibiotic removal

International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 142739 - 142739

Опубликована: Апрель 1, 2025

Язык: Английский

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Lignocellulose deconstruction and mechanistic insights into wheat straw using extrusion-ammoniation synergistic pretreatment

Industrial Crops and Products, Год журнала: 2025, Номер 228, С. 120820 - 120820

Опубликована: Март 18, 2025

Язык: Английский

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