Benzimidazole Fungicide Carbendazim Induces Gut Inflammation through the TLR5/NF-κB Pathway in Grass Carp

Environmental Science & Technology, Год журнала: 2025, Номер unknown

Опубликована: Фев. 2, 2025

Fungicides have been increasingly used across various sectors, including agriculture and textiles. The biocidal properties of fungicides may negatively impact the stability intestinal microbiota, thereby posing a threat to health. In this study, we investigated mechanisms damage functional abnormalities in grass carp following 42-day exposure widely fungicide carbendazim at environmentally relevant concentrations (0.2 20 μg/L). Histopathological observations, mRNA protein expression analyses, biochemical analysis, quantification short-chain fatty acids (SCFAs), cytokines, lipopolysaccharide (LPS), 16S ribosomal ribonucleic acid (rRNA), as well internal transcribed spacer (ITS) sequencing, were performed. At concentrations, strongly induced inflammation, leading increased transcriptional translational levels genes involved toll-like receptor five (TLR5)/nuclear factor kappa B (NF-κB) pathway, TLR5, NF-κB, interleukin-1 beta (IL-1β), tumor necrosis factor-alpha (TNFα). Additionally, damaged barriers reduced tight junction proteins (e.g., occludin zonula occludens-1/2), goblet cells, immunoglobulin M levels, while also disrupting gut microbiome, metabolic disorders, particularly decreases SCFAs increases LPS. Treatment with TLR5 antagonist TH1020 mitigated inflammation caused by carbendazim, subsequently improving mechanical barrier function. Overall, our findings provide new insights into toxicological underlying carp, indicating that poses significant nontarget organisms. Given its widespread detection environment, these results underscore substantial ecological risks health fish living carbendazim-contaminated water bodies.

Язык: Английский

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Assessing the chronic toxicity of climbazole to Daphnia magna: Physiological, biochemical, molecular, and reproductive perspectives

Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology, Год журнала: 2024, Номер 287, С. 110061 - 110061

Опубликована: Окт. 20, 2024

Язык: Английский

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Visible light-driven activation of persulfate over ferrous titanate/thermally exfoliated carbon nitride S-scheme heterojunction for boosting the degradation of climbazole

Separation and Purification Technology, Год журнала: 2025, Номер unknown, С. 131803 - 131803

Опубликована: Янв. 1, 2025

Язык: Английский

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In Vitro Exposure to the Endocrine-Disrupting Chemical Climbazole Impairs Human Sperm Motility, Hormonal Signalling, and Mitochondrial Activity

Cells, Год журнала: 2025, Номер 14(6), С. 427 - 427

Опубликована: Март 13, 2025

This study explores the endocrine-disrupting effects of climbazole (CBZ), an environmental and lifestyle stressor, on male fertility. The impact CBZ sperm vitality, motility, molecular pathways related to hormone receptors apoptosis was evaluated, in non-capacitated capacitated conditions. Gene expression key components, including (ESR1, ESR2, FSHR, AR), apoptosis-related genes (BAX, BCL2), COX4l1 (involved mitochondrial function), analyzed. Protein tyrosine phosphorylation, a marker capacitation, also examined using immunofluorescence Western blot analysis. We demonstrated that significantly reduced vitality at concentrations above 25 µM motility 1 10 Changes phosphorylation patterns were observed. analysis revealed upregulation ESR1, BAX, while AR downregulated. These findings offer new insights into potential cytotoxic CBZ, highlighting its role compromising reproductive health.

Язык: Английский

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Toxicological effects of chemical pesticides in fish: Focusing on intestinal injury and gut microbial dysbiosis

Pesticide Biochemistry and Physiology, Год журнала: 2025, Номер 211, С. 106405 - 106405

Опубликована: Апрель 9, 2025

Язык: Английский

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Ecotoxicological Impacts of Polystyrene Microplastics on Rainbow Trout: A Multidisciplinary Analysis of Gut Microbiota Dysbiosis, Oxidative Stress, and Cellular Senescence for Environmental Risk Assessment

Process Safety and Environmental Protection, Год журнала: 2025, Номер unknown, С. 107323 - 107323

Опубликована: Май 1, 2025

Язык: Английский

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Enantioselective disruption of circadian rhythm behavior in goldfish (Carassius auratus) induced by chiral fungicide triadimefon at environmentally-relevant concentration

Journal of Hazardous Materials, Год журнала: 2024, Номер 485, С. 136891 - 136891

Опубликована: Дек. 15, 2024

Язык: Английский

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Long-term exposure to climbazole may affect the health of stress-tolerant coral Galaxea fascicularis

Marine Environmental Research, Год журнала: 2024, Номер 201, С. 106679 - 106679

Опубликована: Авг. 10, 2024

Язык: Английский

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Toxic effects of exposure to Polymethyl methacrylate and polyvinyl chloride microplastics in Pacific oysters (Crassostrea gigas)

Environmental Pollution, Год журнала: 2024, Номер unknown, С. 125484 - 125484

Опубликована: Дек. 1, 2024

Язык: Английский

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Concentration-Dependent Effect of Norfloxacin on Fe Transport, Energy Metabolism, and Immunity in the Intestine of Large Yellow Croaker

Опубликована: Янв. 1, 2024

This study explores the effects of norfloxacin (NOR) on oxidative damage, iron (Fe) transport, energy metabolism, and immunotoxicity in intestine large yellow croaker under Fe stress. The fish were subjected to (180 μg/L), low-dose NOR (1.8 μg/L, LNOR), high-dose HNOR), plus LNOR, HNOR for 60 days. Our results demonstrated that alone exposure increased malondialdehyde (MDA) protein carboxylation (PC) contents, mortality rate, impaired intestinal tissue, which was related increment accumulation. Compared exposure, LNOR decreased MDA PC alleviated malformations caused by stress, indicating had an antagonistic effect toxicity. associated with enhancement storage excretion, anti-inflammatory response, heat stress proteins (HSPs) gene expressions, reduction pro-inflammatory response. elevated deteriorated Fe-induced malformations, a synergetic between inhibition HSP pro-inflammatory. Metal regulatory transcription factor 1 (Mtf1) nuclear kappa B (NF-κB) expression significant correlations transport immunity mRNA levels, respectively, suggesting Mtf1 NF-κB participated modulation expressions. data clearly showed dose-dependent Fe-toxicity croaker, contributes revealing mechanism affecting physiology

Язык: Английский

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