
The Science of The Total Environment, Год журнала: 2025, Номер 979, С. 179516 - 179516
Опубликована: Апрель 24, 2025
The spatial distribution of soil bacterial communities in agrosilvopastoral systems remains understudied, despite its fundamental role ecosystem functioning. This study investigates the dynamics dominant copiotrophic and oligotrophic phyla grazing areas Southwest Spain, focusing on their interactions with land management, properties, environmental covariates. Five management systems; occasional (OG), holistic (HM), organic farming (OF), conventional rangeland (CR), grassland (CG) were analyzed across three topographic positions (hilltop, mid-slope, valley bottom), representing a gradient intensity. A total 71 samples collected using 16S rRNA metabarcoding. Alpha beta diversity metrics revealed significant shifts community composition driven by both topography, HM showing higher richness compared to CR CG. Among phyla, groups such as Proteobacteria Actinobacteriota more abundant upper slope under intensity, whereas Verrucomicrobiota was enriched bottoms lower pressure. Spatial prediction models based Random Forest recursive feature elimination (RFE) identified key drivers, vegetation indices being relevant for Verrucomicrobiota, features Actinobacteriota. RDA SEM confirmed that animal stocking rate matter major predictors β-diversity. provides novel insights into microbial heterogeneity Mediterranean systems, highlighting interplay practices, characteristics, topography. findings underscore ecological benefits enhancing inform strategies sustainable use ecosystems.
Язык: Английский