Genome-Wide Analysis of Genetic Predispositions Linked to Damaged Membranes and Impaired Fertility as Indicators of Compromised Sperm–Egg Interaction Mechanisms in Frozen–Thawed Rooster Semen

Frontiers in Bioscience-Scholar, Год журнала: 2025, Номер 17(1)

Опубликована: Янв. 22, 2025

Background: Cryopreservation cannot be widely used for rooster sperm due to high incidences of cryoinjury, including damage membranes. Thus, cryopreserved has limited use low motility and reduced fertilizing ability, which disrupts the mechanisms involved in sperm–egg interactions. Previously, we an Illumina 60K single-nucleotide polymorphism (SNP) array search genes associated with quality, before after freeze–thawing. As a continuation these genome-wide association studies (GWAS), present investigation denser 600K SNP chip. Consequently, screen depth was expanded by many markers cryo-resistance while more candidate were identified. our study aimed identify associations ejaculate quality indicators, those concerning membrane damage. Methods: We selected indicators freezing–thawing using samples from proprietary cryobank collection created preserve generative germ cells rare endangered breeds chickens other animal species. A total 258 ejaculates 96 roosters 16 different analyzed. Moreover, respective DNA isolated genotyping Affymetrix® Axiom® high-density array. Results: In total, 31 SNPs 26 characteristics damage, progressive motility, cell respiration induction 2,4-dinitrophenol. particular, identified ENSGALG00000029931 gene as PHF14 ARID1B damaged membranes, KDELR3, DDX17, DMD, CDKL5, DGAT2, ST18, FAM150A, DIAPH2, MTMR7, NAV2, RAG2, PDE11A, IFT70A, AGPS, WDFY1, DEPDC5, TSC1, CASZ1, PLEKHM2 induction. Conclusions: Our findings provide important information understanding genetic basis integrity traits that can potentially compromise These are relevant persistence fertility thawing previously frozen semen.

Язык: Английский

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Designing Flexible SERS Platforms by embedding Double-Plasmonic (Ag-Au) Nanostructures in Porous ZIF-8 Frameworks on PDMS

Sensors and Actuators A Physical, Год журнала: 2025, Номер unknown, С. 116423 - 116423

Опубликована: Март 1, 2025

Язык: Английский

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Thiram-induced ER stress promotes mitochondrial calcium signaling and NLRP3 inflammasome activation in a tissue specific manner

Ecotoxicology and Environmental Safety, Год журнала: 2025, Номер 293, С. 118026 - 118026

Опубликована: Март 1, 2025

Thiram, a broadly used dithiocarbamate fungicide, exaggerates endoplasmic reticulum (ER) stress and interferes with mitochondrial function, thus disrupting cellular homeostasis. Here, we intend to identify the molecular actions of thiram at mitochondrial-associated ER membranes (MAMs) that lead induction calcium overload in both liver bone tissues. Taken together, show thiram-induced remodelling MAMs leads huge dysregulation. Histological immunohistochemical examinations revealed hyperactivation IP3R1 mediated release calcium, but uptake was by voltage-dependent anion channels VDAC1. This response characterized increased glucose regulated protein 78 (GRP78) expression tibial growth plates (GP). In this respect, new liver-bone axis delineated for stress. More interestingly, activation NLRP3 inflammasome very striking not Hence, results highlight systemic effects identifying critical metabolic junction might play role disorders such as dyschondroplasia related disorders, e.g., osteoarthritis osteoporosis.

Язык: Английский

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Chondroitin sulfate reverses tibial dyschondroplasia, broiler chondrocyte proliferation and differentiation dysfunction via the CHST11/β-Catenin pathway

International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 144488 - 144488

Опубликована: Май 1, 2025

Язык: Английский

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Transcriptome analysis reveals the pathogenesis of spontaneous tibial dyschondroplasia in broilers

Frontiers in Genetics, Год журнала: 2024, Номер 15

Опубликована: Июль 29, 2024

Tibial dyschondroplasia (TD) is a severe bone disease that affects fast-growing broiler chickens and causes economic loss. Despite previous studies, the regulatory mechanism of TD remains unclear thought to be primarily based on thiram induction, which may differ from naturally occurring diseases. To better understand TD, digital X-ray machine was used in present study determine its incidence four hundred yellow-feathered chickens. The results showed 22% after 6 weeks gradually decreased 8 10 weeks. body weight broilers with significantly compared NTD broilers. In addition, length density tibia were reduced eight weeks, This also examined tibial quality parameters (n = 12) found mineral content, density, ash calcium phosphorus content Transcriptome analysis revealed 849 differentially expressed genes (DEGs) growth plate between 6) groups 6). These enriched ECM-receptor interaction, cytokine-cytokine receptor signaling pathway, TGF-β signaling. Genes encoding alpha chain type XII collagen, is,

Язык: Английский

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Simple and rapid capillarity-assisted ultra-trace detection of thiram on apple surface with a silver nanoparticles/filter paper SERS substrate

Journal of Hazardous Materials, Год журнала: 2024, Номер 485, С. 136945 - 136945

Опубликована: Дек. 19, 2024

Язык: Английский

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