Transcriptomic and metabolomic analyses provide insights into the energy metabolism and signaling regulation of byssus secretion in winged pearl oyster Pteria penguin

Comparative Biochemistry and Physiology Part D Genomics and Proteomics, Год журнала: 2025, Номер 54, С. 101446 - 101446

Опубликована: Фев. 14, 2025

Язык: Английский

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Integrated transcriptomic and epigenomic analyses to disclose the transcriptional regulatory mechanisms of lipid and energy metabolism under cold stress in grass carp

Aquaculture, Год журнала: 2024, Номер 595, С. 741526 - 741526

Опубликована: Авг. 26, 2024

Язык: Английский

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Biochemical, Histological, and Multi-Omics Analyses Reveal the Molecular and Metabolic Mechanisms of Cold Stress Response in the Chinese Soft-Shelled Turtle (Pelodiscus sinensis)

Biology, Год журнала: 2025, Номер 14(1), С. 55 - 55

Опубликована: Янв. 11, 2025

The Chinese soft-shelled turtle (Pelodiscus sinensis), a type of warm-water reptile, is frequently chosen as the model animal to understand how organisms respond environmental stressors. However, responsive mechanism P. sinensis natural cold stress unclear, especially in terms metabolic pattern and molecular pathways. Herein, plasma biochemical, hepatic morphological, apoptotic, transcriptomic, metabolomic detection methods were performed investigate response acute stress. A consistent increase AST ALT activities with decline ALP activity was found following 14 °C 7 compared control group. Plasma GLU, TG, CHO, HDL contents, reflecting energy metabolism, decreased lower levels from 2 16 days post (dps). Histological TUNEL liver demonstrated that caused severe morphological damage cell apoptosis time-dependent manner. DEGs biosynthesis fatty acids (Acsbg2, Acsl3, Acsl4, Acsl5, Mcat, Acacb), well unsaturated (Hsd17b12, Elovl7, Scd, Baat), starch sucrose metabolism (Pgm1, Pgm2, Treh), (Ddit3, Gadd45a, Lmnb1, Tuba1c, Tnf, Tnfsf10, Fos, Itpr1, Ctso) discovered transcriptome under data showed metabolites, including chenodeoxycholic acid, oleoylethanolamide, uric fructose 1,6-bisphosphate, CMP, S-(Hydroxymethyl)-glutathione, remarkably altered groups. Combined transcriptomic revealed pyrimidine amino acid pyruvate most significant pathways regulated by low-temperature exposure. Overall, this work suggests could induce obvious at 4 dps. Moreover, main signaling for sinensis.

Язык: Английский

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Comparative transcriptomic analysis reveals the gut-brain axis regulatory mechanisms in cold-tolerant pufferfish (Takifugu fasciatus) under cold stress

Aquaculture, Год журнала: 2024, Номер unknown, С. 742033 - 742033

Опубликована: Дек. 1, 2024

Язык: Английский

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Dietary lecithin attenuates adverse effects of high fat diet on growth performance, lipid metabolism, endoplasmic reticulum stress and antioxidant capacity in the intestine of largemouth bass (Micropterus salmoides)

Aquaculture, Год журнала: 2024, Номер unknown, С. 741688 - 741688

Опубликована: Сен. 1, 2024

Язык: Английский

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Integration of transcriptome, histopathology, and physiological indicators reveals regulatory mechanisms of largemouth bass (Micropterus salmoides) in response to carbonate alkalinity stress

Aquaculture, Год журнала: 2024, Номер unknown, С. 741883 - 741883

Опубликована: Ноя. 1, 2024

Язык: Английский

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Integrated Analysis of Transcriptome and Metabolome in the Brain After Cold Stress of Red Tilapia During Overwintering

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(24), С. 13372 - 13372

Опубликована: Дек. 13, 2024

Cold stress during overwintering is considered a bottleneck problem limiting the development of red tilapia (Oreochromis spp.) industry, and regulation mechanism currently not well understood. In this study, fish (initial weight: 72.71 ± 1.32 g) were divided into cold group (cold) control (normal) group. group, water temperature was maintained at 20 °C, which basically consistent with in greenhouses local areas. decreased from °C to 8 by 2 per day experiment. At end experiment, levels serum urea nitrogen, glucose, norepinephrine, alkaline phosphatase, total bilirubin, cholesterol changed significantly compared that (P < 0.05). Then transcriptome sequencing LC–MS metabolome brain tissue further employed obtain mRNA metabolite datasets. We found FoxO signaling pathway ABC transporters played an important role transcriptome–metabolome association analysis. pathway, differentially expressed genes related cell cycle regulation, apoptosis immune-regulation, oxidative resistance DNA repair. ATP-binding cassette (ABC) subfamily abca, abcb, abcc gene expression levels, deoxycytidine, L-lysine, L-glutamic acid, L-threonine, ornithine, uridine contents changed. Our results suggested may promote through pathway. The respond regulating amino acid metabolism. provided comprehensive understanding overwintering, will facilitate breeding new cold-resistant varieties future.

Язык: Английский

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Transcriptome, histology, and enzyme activities analysis of liver in Phoxinus lagowskii to the low temperature stress and recovery

Comparative Biochemistry and Physiology Part D Genomics and Proteomics, Год журнала: 2024, Номер 52, С. 101317 - 101317

Опубликована: Авг. 24, 2024

Язык: Английский

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