pBI143 as a potential human-specific microbial source tracking marker: Performance evaluation, treatment reduction, and application to river water samples in Japan DOI Creative Commons
Bikash Malla, Sadhana Shrestha, Eiji Haramoto

и другие.

The Science of The Total Environment, Год журнала: 2024, Номер 957, С. 177468 - 177468

Опубликована: Ноя. 14, 2024

Plasmid pBI143, a potential human-specific microbial source tracking marker, has previously been tested only in the USA. This study evaluated performance of pBI143 marker comparison with BacHum, gyrB, and HF183 TaqMan using quantitative PCR on 65 fecal-source samples. Furthermore, it examined reduction by wastewater treatment processes compared to other markers. The analysis included 48 samples for TaqMan, along 17 additional samples, raw secondary-treated sewage (n = 2 each) deer fecal 13). validation results indicated that is highly sensitive specific detecting human contamination, sensitivity, specificity, accuracy 100 %, 88 94 respectively. outperformed markers, which, despite having high sensitivity (97 %-100 %), exhibited much lower specificity (44 %-66 %). presence was river water from 21 sites Yamanashi Prefecture detected 95 % indicating likely these are contaminated sources. During treatment, showed significant reduction, highest log

Язык: Английский

Evaluation of plasmid pBI143 for its optimal concentration methods, seasonal impact, and potential as a normalization parameter in wastewater-based epidemiology DOI Creative Commons
Bikash Malla, Sadhana Shrestha, Niva Sthapit

и другие.

The Science of The Total Environment, Год журнала: 2025, Номер 965, С. 178661 - 178661

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

1
Evaluation of tomato brown rugose fruit virus as a microbial source tracking marker for human sewage in Thailand DOI

Phongsawat Paisantham,

Supitchaya Theplhar,

Thitima Srathongneam

и другие.

The Science of The Total Environment, Год журнала: 2025, Номер 963, С. 178419 - 178419

Опубликована: Янв. 17, 2025

Язык: Английский

Процитировано

0
pBI143 as a potential human-specific microbial source tracking marker: Performance evaluation, treatment reduction, and application to river water samples in Japan DOI Creative Commons
Bikash Malla, Sadhana Shrestha, Eiji Haramoto

и другие.

The Science of The Total Environment, Год журнала: 2024, Номер 957, С. 177468 - 177468

Опубликована: Ноя. 14, 2024

Plasmid pBI143, a potential human-specific microbial source tracking marker, has previously been tested only in the USA. This study evaluated performance of pBI143 marker comparison with BacHum, gyrB, and HF183 TaqMan using quantitative PCR on 65 fecal-source samples. Furthermore, it examined reduction by wastewater treatment processes compared to other markers. The analysis included 48 samples for TaqMan, along 17 additional samples, raw secondary-treated sewage (n = 2 each) deer fecal 13). validation results indicated that is highly sensitive specific detecting human contamination, sensitivity, specificity, accuracy 100 %, 88 94 respectively. outperformed markers, which, despite having high sensitivity (97 %-100 %), exhibited much lower specificity (44 %-66 %). presence was river water from 21 sites Yamanashi Prefecture detected 95 % indicating likely these are contaminated sources. During treatment, showed significant reduction, highest log

Язык: Английский

Процитировано

1