Oligoasthenozoospermia is alleviated in a mouse model by [Gly14]‐humanin‐mediated attenuation of oxidative stress and ferroptosis DOI Open Access
Yumeng Liu, Liping Zou, Liping Zou

и другие.

Andrology, Год журнала: 2024, Номер unknown

Опубликована: Окт. 22, 2024

Abstract Background Oligoasthenozoospermia is a common cause of male infertility, for which effective treatments are urgently needed. Humanin (HN) peptide associated with this condition. Objectives To investigate the ameliorative effect [Gly14]‐Humanin (HNG) on oligoasthenozoospermia and mechanisms. Materials methods Mice were treated cyclophosphamide (CP) to construct mice model oligoasthenozoospermia. The resulting saline or HNG. Subsequently, testis weights, organ indices, testicular structure, sperm counts motilities, litter sizes, serum testosterone concentrations determined. Differential gene expression in tissues was determined by RNA sequencing. TM3, TM4, GC1, GC2 cells exposed erastin induce ferroptosis, followed treatment HNG + ML385 (a nuclear factor erythroid 2‐related 2 inhibitor). Levels reactive oxygen species (ROS), malondialdehyde (MDA), glutathione (GSH), ferrous ions (Fe 2+ ) their ferroptosis‐related proteins immunofluorescence western blot. Results improved parameters increased size mice. Kyoto Encyclopaedia Genes Genomes pathway enrichment analysis revealed significant differential genes. after treatment. ROS, MDA, Fe decreased GSH TM3 TM4 In vitro experiments confirmed that activated 2/glutathione peroxidase 4 (Nrf2/GPX4) pathway. However, these effects blocked Discussion conclusion demonstrated therapeutic mouse reducing oxidative stress ferroptosis. cells, attenuated cellular inhibited ferroptosis via Nrf2/GPX4

Язык: Английский

Research Progress in Reproductive Toxicity of Micro- and Nanoplastics on Males and Its Mechanisms DOI Creative Commons

昱 谭

Hans Journal of Food and Nutrition Science, Год журнала: 2025, Номер 14(01), С. 23 - 29

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0

Micro-nanoplastics pollution and mammalian fertility: a systematic review and meta-analysis DOI Creative Commons

Alessandro Marino Volsa,

Eleonora Iacono, Barbara Merlo

и другие.

Theriogenology, Год журнала: 2025, Номер 238, С. 117369 - 117369

Опубликована: Фев. 26, 2025

Язык: Английский

Процитировано

0

Impacts of iodoacetic acid on reproduction: current evidence, underlying mechanisms, and future research directions DOI Creative Commons
Mei Ha,

Li Mou,

Jiayuan Qu

и другие.

Frontiers in Public Health, Год журнала: 2024, Номер 12

Опубликована: Окт. 3, 2024

In light of the undeniable and alarming fact that human fertility is declining, harmful factors affecting reproductive health are garnering more attention. Iodoacetic acid (IAA), an emerging unregulated drinking water disinfection byproduct, derives from chlorine frequently detected in environment biological samples. Humans ubiquitously exposed to IAA daily mainly through water, consuming food beverages made disinfected contacting swimming pools bath etc. Mounting evidence has indicated could act as a toxicant bring about multifarious adverse damage. For instance, it can interfere with gonadal development, weaken ovarian function, impair sperm motility, trigger DNA damage germ cells, perturb steroidogenesis, The underlying mechanisms predominantly include cytotoxic genotoxic effects on disturbance hypothalamic-pituitary-gonadal axis, oxidative stress, inhibition steroidogenic proteins or enzymes, dysbiosis gut microbiota. Nevertheless, there still some knowledge gaps limitations studying potential impact reproduction, which urgently need be addressed future. We suppose necessary population epidemiological studies, sensitive detection methods for internal exposure, mechanism-based in-depth exploration will contribute comprehensive understanding characteristics IAA, thus providing important scientific basis revising sanitary standards quality.

Язык: Английский

Процитировано

1

Oligoasthenozoospermia is alleviated in a mouse model by [Gly14]‐humanin‐mediated attenuation of oxidative stress and ferroptosis DOI Open Access
Yumeng Liu, Liping Zou, Liping Zou

и другие.

Andrology, Год журнала: 2024, Номер unknown

Опубликована: Окт. 22, 2024

Abstract Background Oligoasthenozoospermia is a common cause of male infertility, for which effective treatments are urgently needed. Humanin (HN) peptide associated with this condition. Objectives To investigate the ameliorative effect [Gly14]‐Humanin (HNG) on oligoasthenozoospermia and mechanisms. Materials methods Mice were treated cyclophosphamide (CP) to construct mice model oligoasthenozoospermia. The resulting saline or HNG. Subsequently, testis weights, organ indices, testicular structure, sperm counts motilities, litter sizes, serum testosterone concentrations determined. Differential gene expression in tissues was determined by RNA sequencing. TM3, TM4, GC1, GC2 cells exposed erastin induce ferroptosis, followed treatment HNG + ML385 (a nuclear factor erythroid 2‐related 2 inhibitor). Levels reactive oxygen species (ROS), malondialdehyde (MDA), glutathione (GSH), ferrous ions (Fe 2+ ) their ferroptosis‐related proteins immunofluorescence western blot. Results improved parameters increased size mice. Kyoto Encyclopaedia Genes Genomes pathway enrichment analysis revealed significant differential genes. after treatment. ROS, MDA, Fe decreased GSH TM3 TM4 In vitro experiments confirmed that activated 2/glutathione peroxidase 4 (Nrf2/GPX4) pathway. However, these effects blocked Discussion conclusion demonstrated therapeutic mouse reducing oxidative stress ferroptosis. cells, attenuated cellular inhibited ferroptosis via Nrf2/GPX4

Язык: Английский

Процитировано

0