Evaluation of the effects of carbamazepine-loaded chitosan-coated PLGA-Zein nanoparticles on pilocarpine-induced seizure model in zebrafish larvae: Developmental toxicity and behavioral assays DOI Creative Commons
Gonca Alak, Serkan Yıldırım, Afife Büşra Uğur Kaplan

и другие.

Environmental Toxicology and Chemistry, Год журнала: 2025, Номер 44(3), С. 751 - 763

Опубликована: Янв. 17, 2025

Abstract Epilepsy, the most common neurological disorder worldwide, is characterized by sudden paroxysmal brain activity, which can be generalized or focal. Extensive research has explored various treatment strategies for this condition. Our study used a pilocarpine (PL)-induced seizure model in zebrafish (Danio rerio) embryos and larvae to assess effects of carbamazepine (CBZ)-loaded chitosan-coated PLGA-Zein nanoparticles (NPs) over 96 hr. We evaluated developmental toxicity (mortality, malformation, larval hatching), behavioral changes (sensorimotor reflexes), histopathological immunohistochemical alterations tissue, focusing on 5-hydroxytryptamine receptor 4 (5HT4), muscle ARNT-like 1 (BMAL1) expressions. findings revealed high mortality malformation rates groups treated with pure CBZ (PL + 50 PL 100). These also exhibited delayed hatching impaired sensorimotor reflexes. In contrast, CBZ-NP-treated NP 100) showed comparable control group, significantly lower compared CBZ-treated groups. Moreover, intense cytoplasmic expression 5HT4 BMAL1 was observed neuropils 100 group. This highlights potential CBZ-loaded NPs reducing adverse associated models.

Язык: Английский

Evaluation of the effects of carbamazepine-loaded chitosan-coated PLGA-Zein nanoparticles on pilocarpine-induced seizure model in zebrafish larvae: Developmental toxicity and behavioral assays DOI Creative Commons
Gonca Alak, Serkan Yıldırım, Afife Büşra Uğur Kaplan

и другие.

Environmental Toxicology and Chemistry, Год журнала: 2025, Номер 44(3), С. 751 - 763

Опубликована: Янв. 17, 2025

Abstract Epilepsy, the most common neurological disorder worldwide, is characterized by sudden paroxysmal brain activity, which can be generalized or focal. Extensive research has explored various treatment strategies for this condition. Our study used a pilocarpine (PL)-induced seizure model in zebrafish (Danio rerio) embryos and larvae to assess effects of carbamazepine (CBZ)-loaded chitosan-coated PLGA-Zein nanoparticles (NPs) over 96 hr. We evaluated developmental toxicity (mortality, malformation, larval hatching), behavioral changes (sensorimotor reflexes), histopathological immunohistochemical alterations tissue, focusing on 5-hydroxytryptamine receptor 4 (5HT4), muscle ARNT-like 1 (BMAL1) expressions. findings revealed high mortality malformation rates groups treated with pure CBZ (PL + 50 PL 100). These also exhibited delayed hatching impaired sensorimotor reflexes. In contrast, CBZ-NP-treated NP 100) showed comparable control group, significantly lower compared CBZ-treated groups. Moreover, intense cytoplasmic expression 5HT4 BMAL1 was observed neuropils 100 group. This highlights potential CBZ-loaded NPs reducing adverse associated models.

Язык: Английский

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