Control of Fusarium graminearum Infection in Wheat by dsRNA-Based Spray-Induced Gene Silencing DOI
Xianzhong Feng,

Yini Shi,

Zhongke Sun

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Апрель 3, 2025

Spray-induced gene silencing (SIGS) has become a new technology for pest and disease control in plants. This study synthesized three double-strand RNAs (dsRNAs) targeting Fusarium graminearum (F. graminearum), the major pathogen causing head blight (FHB). Co-incubation showed weak uptake of dsRNA by F. graminearum, some dsRNAs influence spore germination hyphae growth. In contrast, exogenous quickly efficiently penetrates wheat leaves. Treatment leaves detached heads with these negative effect on pathogenicity graminearum. Foliar spraying dsCHS3b or dsMGV1 decreased amount artificially inoculated incidence rate, severity field. Under natural conditions, significantly FHB index deoxynivalenol content. Twice spray achieved more than 90% FHB. conclusion, SIGS effectively prevents infection wheat, providing green way control.

Язык: Английский

Silencing of the plant‐derived horizontally transferred gene BtSC5DL effectively controls Bemisia tabaci MED DOI Open Access
Yifan Liu, Gong Cheng, Yuan Hu

и другие.

Pest Management Science, Год журнала: 2025, Номер unknown

Опубликована: Янв. 11, 2025

Abstract BACKGROUND The whitefly Bemisia tabaci is a notorious agricultural pest known for its ability to cause significant crop damage through direct feeding and virus transmission. Its remarkable adaptability reproductive capacity are linked acquire integrate horizontally transferred genes (HTGs) into genome. These HTGs increase the physiological metabolic capacities of this pest, including cholesterol synthesis, which critical survival success. Among these genes, we identified plant‐derived B. Δ7‐sterol C5‐desaturase‐like gene ( BtSC5DL ), plays pivotal role in metabolism biology. RESULTS In study, cloned from Mediterranean (MED). Bioinformatics molecular analyses revealed that was plants millions years ago now stably expressed species. Silencing dsRNA resulted reductions egg production content MED. Furthermore, virus‐induced silencing (VIGS) experiments confirmed long‐term suppression had notable control populations. CONCLUSION Our results demonstrate crucial biosynthesis MED suggest acquisition significantly enhances findings provide theoretical basis development RNA interference (RNAi)‐based strategies targeting , offering potential new approach effective management populations settings. © 2025 Society Chemical Industry.

Язык: Английский

Процитировано

2

Investigating the Biology of Leaf-Cutting Ants to Support the Development of Alternative Methods for the Control and Management of These Agricultural Pests DOI Creative Commons
Virginia E. Masiulionis, Richard Ian Samuels

Agriculture, Год журнала: 2025, Номер 15(6), С. 642 - 642

Опубликована: Март 18, 2025

Concerns about the environmental and health risks of synthetic insecticides are driving search for alternative pest control methods. Leaf-cutting ants (LCAs), one most significant pests in neotropics, cause substantial economic damage to agriculture present challenges due their complex biology ecology. While chemical remains primary strategy, its intensive use has negative impacts, promotes resistance, endangers non-target species, including plants, animals, humans. This review describes LCAs, examines traditional methods suggests strategies such as entomopathogenic fungi (EPFs) combined with sublethal doses insecticides, plant essential oils (EOs), RNAi techniques. Here, we emphasize need address LCA management sustainably by investigating ecology at both “colony” “individual” levels. Colony-level factors include morphology, life cycle, behavior, division labor, nest structure, while individual-level mechanisms involve sensory, biochemical, behavioral adaptations garden sterilization decontamination. also highlights potential insecticide EPFs induce changes worker mortality, it details mode action EOs promising strategies. The integration biological approaches could offer sustainable alternatives insecticides.

Язык: Английский

Процитировано

0

Control of Fusarium graminearum Infection in Wheat by dsRNA-Based Spray-Induced Gene Silencing DOI
Xianzhong Feng,

Yini Shi,

Zhongke Sun

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Апрель 3, 2025

Spray-induced gene silencing (SIGS) has become a new technology for pest and disease control in plants. This study synthesized three double-strand RNAs (dsRNAs) targeting Fusarium graminearum (F. graminearum), the major pathogen causing head blight (FHB). Co-incubation showed weak uptake of dsRNA by F. graminearum, some dsRNAs influence spore germination hyphae growth. In contrast, exogenous quickly efficiently penetrates wheat leaves. Treatment leaves detached heads with these negative effect on pathogenicity graminearum. Foliar spraying dsCHS3b or dsMGV1 decreased amount artificially inoculated incidence rate, severity field. Under natural conditions, significantly FHB index deoxynivalenol content. Twice spray achieved more than 90% FHB. conclusion, SIGS effectively prevents infection wheat, providing green way control.

Язык: Английский

Процитировано

0