BioResources,
Год журнала:
2023,
Номер
19(1), С. 842 - 857
Опубликована: Дек. 11, 2023
Polyphenols
were
extracted
from
Thuja
occidentalis
L.
bark
and
characterized
in
terms
of
phytochemical
composition
biological
activity.
The
optimization
process
investigated
the
impacts
extraction
duration,
ultrasound
amplitude,
ethanol
concentration
on
total
phenolic
content
extract.
antioxidant
capacity
was
examined
using
DPPH
ABTS
assays,
anti-enzyme
activity
against
alpha-glucosidase,
alpha-amylase,
lipase,
acetylcholinesterase,
tyrosinase
determined.
experimental
model
revealed
optimal
parameters:
a
hydroethanolic
solvent
with
44%
v:v
ethanol,
15-minute
time,
40%
amplitude.
These
parameters
validated
confirmed
by
reaching
target
Total
Phenolic
Content
(TPC)
predicted
MODDE
software.
resulting
lyophilized
extract
exhibited
high
polyphenolic
(161.84
±
23.48
mg
GAE/g
dry
extract)
demonstrated
strong
properties.
Notably,
it
showed
significant
inhibition
alpha-glucosidase
suggesting
potential
antidiabetic
effects,
though
its
relatively
weak.
findings
provide
foundation
for
further
investigations
to
confirm
qualitative
quantitative
presence
specific
metabolites
elucidate
mechanisms
underlying
bioactive
