The long noncoding RNA noncoding RNA activated by DNA damage (NORAD)-microRNA-496-Interleukin-33 axis affects carcinoma-associated fibroblasts-mediated gastric cancer development

Bioengineered, Год журнала: 2021, Номер 12(2), С. 11738 - 11755

Опубликована: Дек. 11, 2021

Carcinoma-associated fibroblasts (CAFs) are one of the crucial parts in tumor microenvironment and contribute to progression. Interleukin-33 (IL-33), a tissue-derived nuclear cytokine from IL-1 family, has been found abnormally expressed cells Fibroblast. However, role mechanism IL-33 interaction between gastric cancer (GC) CAFs need investigation. Presently, we inquire into function lncRNA NORAD-miR-496 axis-mediated modulating GC-CAFs interaction. Real-time reverse transcription-polymerase chain reaction (RT-PCR) was adopted gauge expression NORAD, miR-496, GC tissues cells, gain- or loss-of-function assays were conducted investigate them GC. A cell-CAFs co-culture model established explore GCs. As exhibited, NORAD up-regulated while miR-496 remarkably down-regulated. Overexpressing substantially promoted proliferation, migration, invasion, EMT repressed cell death, overexpressing had opposite effects. Additionally, enhanced release cells. The dual-luciferase reporter assay confirmed that target targeted IL-33. aggravated malignant behaviors as indicated by both experiments. knockdown reversed CAFs-mediated promotive effects on In conclusion, effect up-regulating targeting which provided new insights CAFs.Abbreviation ANOVA: Analysis Variance; BCA:Bicinchoninic acid; CAFs: carcinoma-associated fibroblasts; CCK-8: counting kit-8; ceRNA: competing endogenous RNA; DAPI: 4′,6-diamidino-2-phenylindole; DMEM: Dulbecco's minimal essential medium/Ham's; ECL: chemiluminiscent; ELISA: Enzyme-Linked Immunosorbent Assay; EMT: epithelial-mesenchymal transition; FBS: fetal bovine serum; FISH:Fluorescence situ hybridization; FITC:fluorescein isothiocyanate; FSP:fibroblast-specific protein; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; GC: cancer; IHC: immunohistochemistry; IL: Interleukin; lncRNA: long Noncoding miR-496: microRNA-496; MMP-14:matrix metalloproteinase-14; MUT:mutant; MYH9: myosin heavy 9; NFs: normal NORAD: RNA activated DNA damage; ORF: open reading frame; PBS: phosphate-buffered saline; PMSF: Phenylmethylsulfonyl fluoride; PVDF: polyvinylidene difluoride; RIPA: Radio-Immunoprecipitation RT-PCR: transcription polymerase reaction; S100A4:S100 calcium binding protein A4; SDS-PAGE: sodium dodecyl sulfate-polyacrylamide gel electrophoresis; sh-NC: short-hairpin negative control; sh-NORAD: NORAD; α-SMA: α-smooth muscle actin; TBST: Tris-buffered saline with Tween-20; TGF-β1: Transforming growth factor β1; TUNEL: TdT-mediated dUTP Nick-End Labeling; TWIST1: twist-related 1; VEGF-C: vascular endothelial C; WT: Wildtype.

Язык: Английский

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Matrine Combined with Mammalian Target of Rapamycin Inhibitor Enhances Anti-Tumor Efficacy of dendritic cell Vaccines in hepatocellular carcinoma

Bioengineered, Год журнала: 2022, Номер 13(4), С. 9274 - 9283

Опубликована: Апрель 1, 2022

Dendritic cells (DCs), as the most important antigen-presenting cells, play a crucial role in T cell activation. The latest research showed that inhibition of mammalian target rapamycin (mTOR) could enhance DCs maturation, promoting antigen presentation. Matrine has been identified one key alkaloids isolated from roots Sophora flavescens. In present study, we combined matrine and mTOR inhibitor KU0063794 to observe functions, especially presentation ability. were activated by phosphate-buffered saline (PBS), lipopolysaccharide (LPS), LPS+KU0063794, LPS+Matrine, LPS+KU0063794+Matrine. surface markers DCs, proliferation cytokines detected flow cytometry, counting kit-8 (CCK-8) enzyme-linked immunosorbent assay (ELISA), respectively. lactate dehydrogenase (LDH) release test was used detect antitumor efficacy. tumor growth curves plotted calculating volume. apoptosis Terminal-deoxynucleoitidyl Transferase-Mediated Nick End Labeling (TUNEL) method. with maturity secretion (P < 0.05). cytotoxic lymphocytes (CTL) killing efficacy LPS+KU0063794+Matrine group higher than other groups vivo, weights volumes lower groups. detections increased DC vaccine significantly improve immunity vitro vivo. These findings illustrated matrine, two immunomodulators, activation differentiation.

Язык: Английский

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Identification and validation of an autophagy-related signature for predicting survival in lower-grade glioma

Bioengineered, Год журнала: 2021, Номер 12(2), С. 9692 - 9708

Опубликована: Окт. 26, 2021

Abnormal levels of autophagy have been implicated in the pathogenesis multiple diseases, including cancer. However, little is known about role autophagy-related genes (ARGs) low-grade gliomas (LGG). Accordingly, aims this study were to assess prognostic values ARGs and establish a genetic signature for LGG prognosis. Expression profile data from patients with without primary obtained The Cancer Genome Atlas (TCGA) Tissue databases, respectively, consensus clustering was used identify clusters distinct prognoses. Nineteen differentially expressed selected threshold FDR < 0.05 |log2 fold change (FC)| ≥ 2, functional analysis revealed that these associated processes as expected. An established using Cox regression model six separated TCGA training cohort into high- low-risk groups. Univariate multivariate indicated signature-based risk score an independent factor. successfully validated testing, entire, Chinese Glioma cohorts. Stratified analyses demonstrated clinical features prognosis, gene set enrichment autophagy- cancer-related pathways more enriched high-risk than patients. value expression signature-related also investigated. Thus, present constructed could optimize individualized survival prediction

Язык: Английский

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Inhibin subunit beta A promotes cell proliferation and metastasis of breast cancer through Wnt/β-catenin signaling pathway

Bioengineered, Год журнала: 2021, Номер 12(2), С. 11567 - 11575

Опубликована: Дек. 10, 2021

Emerging evidence has demonstrated that inhibin subunit beta A (INHBA) is dysregulated and plays a critical role in various cancers. With the development of sequencing technology, studies have discovered INHBA overexpressed breast cancer tissues. However, biological roles are still far to clear. In present study, we analyzed expression Cancer Genome Atlas (TCGA) database. Quantitative real-time polymerase chain reaction (qRT-PCR) was conducted assess cell lines. Cell proliferation, invasion epithelial-mesenchymal transition (EMT) were determined by using CCK-8, EdU, Transwell western blot assays. The result showed highly expressed Functional analysis revealed silence or elevation inhibited promoted migration, EMT Wnt/β-catenin signaling pathway-related markers MCF-7 cells. Mechanically, blocking pathway XAV939 reversed promotion effect overexpression on cells' migration invasion. Our findings emphasized may act as an oncogene via activating pathway, which provide potential therapeutic target for treatment cancer.

Язык: Английский

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Small Laccase from Streptomyces coelicolor catalyzed chitosan–pectin blending film for hazardous gas removal

Environmental Technology & Innovation, Год журнала: 2021, Номер 23, С. 101690 - 101690

Опубликована: Июнь 15, 2021

Язык: Английский

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