Journal of Orthopaedic Surgery and Research,
Год журнала:
2023,
Номер
18(1)
Опубликована: Дек. 21, 2023
The
therapeutic
potential
of
Caulis
Spatholobi
(CS)
extracts
against
various
cancers
has
been
well
documented,
yet
its
impact
and
mechanism
in
osteosarcoma
(OS)
remain
unexplored.
This
study
aims
to
elucidate
the
effects
CS
on
growth
metastasis
OS,
along
with
underlying
molecular
mechanism.The
proliferative
two
OS
cell
lines
(Saos-2
U2OS)
was
assessed
using
MTT
colony-formation
assays.
Additionally,
migratory
invasive
capacities
cells
were
investigated
through
Transwell
modulation
CXCR4
expression
by
evaluated
qRT-PCR
Western
blotting.
Furthermore,
influence
activation
PI3K/Akt
signaling
determined
blotting.CS
exhibited
a
dose-
time-dependent
inhibition
proliferation
colony
formation
cells.
Notably,
prominently
observed
Saos-2
U2OS,
treatment
led
dose-dependently
reduction
levels.
Silencing
or
inhibiting
function
diminished
Conversely,
induced
suppression
migration
invasion
counteracted
overexpression.
Mechanistically,
repressed
PI3K/AKT
downregulating
expression.CS
mitigate
CXCR4/PI3K/AKT
signaling-mediated
cells,
thus
might
play
an
anti-tumor
role
OS.
International Immunopharmacology,
Год журнала:
2024,
Номер
139, С. 112686 - 112686
Опубликована: Июль 25, 2024
Chronic
hepatitis
B
(CHB)
virus
infection
remains
a
major
public
health
concern.
In
this
study,
the
diagnostic
capability
of
C-X-C
chemokine
receptor
type
4
promoter
methylation
in
patients
with
CHB-associated
liver
fibrosis/cirrhosis
was
evaluated.
Journal of Asian Natural Products Research,
Год журнала:
2024,
Номер
unknown, С. 1 - 21
Опубликована: Авг. 12, 2024
This
study
aims
to
explore
the
mechanisms
of
inhibitory
effect
kaempferol
on
invasion
and
metastasis
gastric
cancer
(GC)
cells
through
network
pharmacology
prediction
experimental
verification.
It
identifies
core
targets
via
PPI
analysis
finds
that
binds
these
well.
Journal of Orthopaedic Surgery and Research,
Год журнала:
2023,
Номер
18(1)
Опубликована: Дек. 21, 2023
The
therapeutic
potential
of
Caulis
Spatholobi
(CS)
extracts
against
various
cancers
has
been
well
documented,
yet
its
impact
and
mechanism
in
osteosarcoma
(OS)
remain
unexplored.
This
study
aims
to
elucidate
the
effects
CS
on
growth
metastasis
OS,
along
with
underlying
molecular
mechanism.The
proliferative
two
OS
cell
lines
(Saos-2
U2OS)
was
assessed
using
MTT
colony-formation
assays.
Additionally,
migratory
invasive
capacities
cells
were
investigated
through
Transwell
modulation
CXCR4
expression
by
evaluated
qRT-PCR
Western
blotting.
Furthermore,
influence
activation
PI3K/Akt
signaling
determined
blotting.CS
exhibited
a
dose-
time-dependent
inhibition
proliferation
colony
formation
cells.
Notably,
prominently
observed
Saos-2
U2OS,
treatment
led
dose-dependently
reduction
levels.
Silencing
or
inhibiting
function
diminished
Conversely,
induced
suppression
migration
invasion
counteracted
overexpression.
Mechanistically,
repressed
PI3K/AKT
downregulating
expression.CS
mitigate
CXCR4/PI3K/AKT
signaling-mediated
cells,
thus
might
play
an
anti-tumor
role
OS.
