Interdisciplinary cancer research, Год журнала: 2024, Номер unknown
Опубликована: Янв. 1, 2024
Язык: Английский
Obesity Medicine, Год журнала: 2025, Номер unknown, С. 100585 - 100585
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
0
Journal of Nanotheranostics, Год журнала: 2025, Номер 6(1), С. 7 - 7
Опубликована: Фев. 27, 2025
A very aggressive and deadly brain cancer, glioblastoma multiforme (GBM) poses formidable obstacles to effective therapy. Despite advancements in conventional therapies like surgery, chemotherapy, radiation therapy, the prognosis for GBM patients remains poor, with limited survival outcomes. Nanotechnology is gaining popularity as a promising platform managing GBM, offering targeted drug delivery, improved therapeutic efficacy, reduced systemic toxicity. This review offers comprehensive analysis of current approach using nanotechnology-based interventions. study explored various nanocarrier (NC) systems polymeric nanoparticles, liposomes, dendrimers, micelles, mesoporous silica nanoparticles precision well efficacy encapsulating delivering agents tumors. Methods improving delivery into cells are described this study, including novel modalities such convection-enhanced intranasal administration, magnetic hyperthermia, peptide-guided immune liposomes. It also explores influence diabetes obesity on rates, suggesting that glucose levels metformin may improve patient The discussion focuses nanotechnology-enabled highlighting challenges opportunities implementing these technologies clinical practice. highlights potential nanotechnology metabolic modulation transforming treatment strategies. To further understand how factors impact develop innovative treatments mellitus, more necessary.
Язык: Английский
Процитировано
0
European Journal of Pharmacology, Год журнала: 2025, Номер unknown, С. 177343 - 177343
Опубликована: Фев. 1, 2025
Язык: Английский
Процитировано
0
Cells, Год журнала: 2025, Номер 14(6), С. 418 - 418
Опубликована: Март 12, 2025
Respiratory viruses, such as influenza virus, rhinovirus, coronavirus, and respiratory syncytial virus (RSV), continue to impose a heavy global health burden. Despite existing vaccination programs, these infections remain leading causes of morbidity mortality, especially among vulnerable populations like children, older adults, immunocompromised individuals. However, the current therapeutic options for viral are often limited supportive care, underscoring need novel treatment strategies. Autophagy, particularly macroautophagy, has emerged fundamental cellular process in host response infections. This not only supports homeostasis by degrading damaged organelles pathogens but also enables xenophagy, which selectively targets particles degradation enhances defense. viruses have evolved mechanisms manipulate autophagy pathways, using them evade immune detection promote replication. review examines dual role manipulation defense, focusing on complex interplay between autophagy-related pathways. By elucidating mechanisms, we aim highlight potential targeting enhance antiviral responses, offering promising directions development effective treatments against
Язык: Английский
Процитировано
0
FEBS Open Bio, Год журнала: 2025, Номер unknown
Опубликована: Апрель 3, 2025
Macroautophagy/autophagy is a crucial cellular process for degrading and recycling damaged proteins organelles, playing significant role in diseases such as cancer neurodegeneration. Evaluating autophagy flux, which tracks autophagosome formation, maturation, degradation, essential understanding disease mechanisms. Current fluorescence‐based methods are resource‐intensive, requiring advanced equipment expertise, limiting their use clinical laboratories. Here, we introduce non‐fluorescent immunohistochemistry (IHC) method using MAP1LC3/LC3 SQSTM1 core markers flux assessment. LC3 levels reflect whereas degradation decrease the number of its puncta indicate active (i.e., lysosomal turnover). We optimized chromogenic detection diaminobenzidine (DAB) staining developed scoring system based on percentage stained cells. This accessible, cost‐effective enables reliable quantification standard light microscope, bridging gap between experimental research diagnostics. Our protocol allows accurate evaluation fixed tissues, offering practical applications biomedical pathology
Язык: Английский
Процитировано
0
Methods in molecular biology, Год журнала: 2024, Номер unknown
Опубликована: Янв. 1, 2024
Язык: Английский
Процитировано
3
Obesity Surgery, Год журнала: 2024, Номер 34(7), С. 2454 - 2466
Опубликована: Май 15, 2024
Язык: Английский
Процитировано
2
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Июнь 30, 2024
Abstract Macroautophagy/autophagy, a crucial cellular process, is typically measured using fluorescence-based techniques, which can be costly, complex, and impractical for clinical settings. In this paper, we introduce novel, cost-effective, non-fluorescent immunohistochemistry (IHC) method evaluating autophagy flux. This technique, based on antigen-antibody reactions chromogenic detection, provides clear, quantifiable results under standard light microscopy, eliminating the need expensive equipment specialized reagents. Our simplifies technical requirements, making it accessible to routine laboratories research settings with limited resources. By comparing our approach traditional fluorescence methods, demonstrate its superior effectiveness, cost-efficiency, applicability patient samples. innovative technique has potential significantly advance improve diagnostics, offering practical robust tool studying mechanisms in diseases such as cancer neurodegenerative disorders. IHC represents significant step forward flux, more reliable, promise of enhancing understanding treatment autophagy-related diseases.
Язык: Английский
Процитировано
2
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, Год журнала: 2024, Номер 1870(7), С. 167289 - 167289
Опубликована: Июнь 12, 2024
Язык: Английский
Процитировано
1
Biochemistry and Biophysics Reports, Год журнала: 2024, Номер 39, С. 101752 - 101752
Опубликована: Июнь 1, 2024
Язык: Английский
Процитировано
0