RNA G-quadruplexes and stress: emerging mechanisms and functions

Trends in Cell Biology, Год журнала: 2024, Номер 34(9), С. 771 - 784

Опубликована: Фев. 9, 2024

Язык: Английский

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PERK-Olating Through Cancer: A Brew of Cellular Decisions

Biomolecules, Год журнала: 2025, Номер 15(2), С. 248 - 248

Опубликована: Фев. 8, 2025

The type I protein kinase PERK is an endoplasmic reticulum (ER) transmembrane that plays a multifaceted role in cancer development and progression, influencing tumor growth, metastasis, cellular stress responses. activation of represents one the three signaling pathways induced during unfolded response (UPR), which triggered, particular, cells constitutively experience various intracellular extracellular stresses impair folding within ER. can lead to both pro-survival proapoptotic outcomes, depending on context extent ER stress. It helps reprogramming gene expression cells, thereby ensuring survival face oncogenic stress, such as replicative DNA damage, also microenvironmental challenges, including hypoxia, angiogenesis, metastasis. Consequently, contributes initiation, transformation, adaptation microenvironment, chemoresistance. However, sustained cell proliferation promote apoptotic death by interconnected processes, mitochondrial dysfunction, translational inhibition, accumulation stresses, specific induction multifunctional factors, CHOP. dual promoting progression suppression makes it complex target for therapeutic interventions. A comprehensive understanding intricacies pathway their impact essential effective strategies, particularly diseases like cancer, where deregulated most, if not all, solid liquid tumors. This article provides overview knowledge acquired from study animal models lines cultured vitro PERK’s functions thus highlighting potential new avenues could this protein.

Язык: Английский

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Corticosterone effects induced by stress and immunity and inflammation: mechanisms of communication

Frontiers in Endocrinology, Год журнала: 2025, Номер 16

Опубликована: Март 20, 2025

The body instinctively responds to external stimuli by increasing energy metabolism and initiating immune responses upon receiving stress signals. Corticosterone (CORT), a glucocorticoid (GC) that regulates secretion along the hypothalamic-pituitary-adrenal (HPA) axis, mediates neurotransmission humoral regulation. Due widespread expression of receptors (GR), effects CORT are almost ubiquitous in various tissue cells. Therefore, on one hand, is molecular signal activates body’s system during other due chemical properties GCs, anti-inflammatory act as stabilizers control response stress. Inflammation manifestation activation. plays dual roles this process both promoting inflammation exerting As hormone, levels fluctuate with degree duration stress, determining its changes it induces. essential for resist diseases maintain homeostasis, imbalance being key factor development diseases. understanding role mechanisms action immunity crucial. This review addresses important issue summarizes interactions between system.

Язык: Английский

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Alterations in Gene Expression and Alternative Splicing Induced by Plasmid-Mediated Overexpression of GFP and P2RY12 Within the A549 Cell Line

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(7), С. 2973 - 2973

Опубликована: Март 25, 2025

Phenotypic modifications and their effects on cellular functions through the up-regulation of target gene expression have frequently been observed in genetic studies, but unique roles cell lines introduced plasmids influencing these not fully revealed. In this research, we developed two distinct derived from A549 line: one that stably overexpresses GFP another is a polyclonal stable line overexpressing both P2RY12. We then utilized transcriptome sequencing (RNA-seq) technology to screen out differentially expressed genes (DEGs) with differential transcript usage (gDTUs) after overexpression (GFP-OE) P2RY12 (P2RY12-OE). found that, compared A549, there were more than 1700 GFP-OE P2RY12-OE cells, while only 866 DEGs identified cells. Notably, differences relatively minor, over 400 exhibiting changes across all three groups. The functional analysis gDTUs showed they highly enriched pathways associated proliferation migration. summary, performed an extensive profile alternative splicing P2RY12-OE, enhancing our comprehension how function within cells processes control expression.

Язык: Английский

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Proteomic Insights into the Anti-Cancer Mechanisms of Boron-Based Compounds in Prostate Cancer

Food Bioscience, Год журнала: 2025, Номер unknown, С. 106558 - 106558

Опубликована: Апрель 1, 2025

Язык: Английский

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Potential vs challenges of expanding the protein universe with genetic code expansion in eukaryotic cells

Journal of Molecular Biology, Год журнала: 2024, Номер 436(21), С. 168807 - 168807

Опубликована: Сен. 30, 2024

Following decades of innovation and perfecting, genetic code expansion has become a powerful tool for in vivo protein modification. Some the major hurdles that had to be overcome include suboptimal performance GCE-specific translational components host systems, competing cellular processes, unspecific modification proteome limited availability codons reassignment. Although strategies have been developed these challenges, there is critical need further advances. Here we discuss current state-of-the-art technology issues still addressed unleash full potential this method eukaryotic cells.

Язык: Английский

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Oxidative Stress, Transfer RNA Metabolism, and Protein Synthesis

Antioxidants and Redox Signaling, Год журнала: 2023, Номер 40(10-12), С. 715 - 735

Опубликована: Сен. 28, 2023

Oxidative stress refers to excessive intracellular levels of reactive oxygen species (ROS) due an imbalance between ROS production and the antioxidant defense system. Under oxidative conditions, cells trigger various response pathways protect themselves, among which repression messenger RNA (mRNA) translation is one key hallmarks promoting cell survival. This regulation process minimizes cellular energy consumption, enabling survive in adverse conditions promote recovery from stress-induced damage.

Язык: Английский

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A Solitary Stalled 80S Ribosome Prevents mRNA Recruitment to Stress Granules

Biochemistry (Moscow), Год журнала: 2023, Номер 88(11), С. 1786 - 1799

Опубликована: Ноя. 1, 2023

In response to stress stimuli, eukaryotic cells typically suppress protein synthesis. This leads the release of mRNAs from polysomes, their condensation with RNA-binding proteins, and formation non-membrane-bound cytoplasmic compartments called granules (SGs). SGs contain 40S but generally lack 60S ribosomal subunits. It is known that cycloheximide, emetine, anisomycin, ribosome inhibitors block progression 80S ribosomes along mRNA stabilize prevent SG assembly. Conversely, puromycin, which induces premature termination, releases polysomes stimulates SGs. The same effect caused by some translation initiation inhibitors, lead polysome disassembly accumulation in form stalled 48S preinitiation complexes. Based on these other data, it believed trigger for presence extended ribosome-free segments, tend condensates cell. this study, we evaluated ability various small-molecule or stimulate assembly under conditions severe oxidative induced sodium arsenite. Contrary expectations, found ribosome-targeting elongation a specific type, arrest solitary at beginning coding regions do not interfere all subsequent completing leaving transcripts (such as harringtonine, lactimidomycin, T-2 toxin), completely arsenite-induced These observations suggest even single sufficient its recruitment into SGs, formation. We propose entry may be mediated contacts between proteins those subunits remain inaccessible when are associated.

Язык: Английский

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Loss of NAT10 Reduces the Translation of Kmt5a mRNA Through ac4C Modification in Cardiomyocytes and Induces Heart Failure

Journal of the American Heart Association, Год журнала: 2024, Номер 13(20)

Опубликована: Окт. 11, 2024

In the past decade, biological functions of various RNA modifications in mammals have been uncovered. N4-acetylcytidine (ac4C), a highly conserved modification, has implicated human diseases. Despite this, involvement ac4C modification cardiac physiology and pathology remains incompletely understood. NAT10 (N-acetyltransferase 10) stands as sole acetyltransferase known to catalyze modification. This study aims explore role pathology.

Язык: Английский

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Advances and opportunities in methods to study protein translation - A review

International Journal of Biological Macromolecules, Год журнала: 2024, Номер 259, С. 129150 - 129150

Опубликована: Янв. 1, 2024

Язык: Английский

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