ACS Applied Materials & Interfaces,
Год журнала:
2024,
Номер
17(1), С. 867 - 879
Опубликована: Дек. 18, 2024
Enzyme
catalysis
is
a
promising
method
for
producing
chiral
chemicals
with
high
stereoselectivity
under
mild
conditions.
However,
the
traditional
batch
reaction
suffers
from
low
enzyme
stability,
cofactor
recycling,
and
poor
reusability.
Here,
we
present
continuous-flow
using
coimmobilized
dual
enzymes
synthesis
of
γ-/δ-lactones,
which
are
widely
used
in
fragrances
flavors.
Typically,
carbonyl
reductase
mutant
SmCRM5
Serratia
marcescens,
was
by
covalent
binding
BmGDH,
glucose
dehydrogenase
capable
recovering
recycling
NADPH.
After
immobilization,
BmGDH
exhibited
8.9-/8.7-fold
increase
catalytic
efficiency
(kcat/Km)
57-/15-fold
half-life
at
30
°C,
respectively.
We
demonstrated
that
continuous
flow
reactor
showed
higher
rate
space-time
yield
(1586
g·L–1
d–1)
than
free
immobilized
production
(R)-δ-decalactone.
This
can
run
continuously
more
650
h
99%
ee
80%
conversion,
total
volume
exceeds
1500
volumes.
The
robustness
this
system
provides
green
efficient
value-added
chemicals.
Ultrasonics Sonochemistry,
Год журнала:
2024,
Номер
111, С. 107153 - 107153
Опубликована: Ноя. 12, 2024
Millet
protein,
as
a
promising
plant-based
protein
substitute
source,
is
an
excellent
basis
for
essential
amino
acids
compared
to
commonly
consumed
staple
grains.
Compared
with
the
traditional
extraction
process,
ultrasound
has
been
used
enhance
efficiency
of
various
proteins.
To
reveal
mechanism
ultrasound-enhanced
proteins,
adaptive
neuro-fuzzy
inference
system
(ANFIS)
algorithm
and
numerical
simulation
based
on
Fick's
law
were
applied
illustrate
mass
transfer
behavior
millet
proteins
under
different
ultrasonic
conditions
including
solid-liquid
ratios
(S/L
ratios),
pH
acoustic
energy
density
levels
(AED).
The
results
showed
that
AED
dominated
changes
in
effective
diffusion
coefficient
(D
Molecules,
Год журнала:
2024,
Номер
29(21), С. 5153 - 5153
Опубликована: Окт. 31, 2024
Macroporous
resin
is
an
efficient
separation
technology
that
plays
a
crucial
role
in
the
and
purification
of
traditional
Chinese
medicine
(TCM).
However,
application
macroporous
resins
TCM
pharmaceuticals
hindered
by
serious
fouling
caused
complex
materials
used
TCM.
This
study
examines
impact
ultrafiltration
(UF)
membrane
on
adsorption
behavior
extracts.
In
this
paper,
ACS Applied Materials & Interfaces,
Год журнала:
2024,
Номер
17(1), С. 867 - 879
Опубликована: Дек. 18, 2024
Enzyme
catalysis
is
a
promising
method
for
producing
chiral
chemicals
with
high
stereoselectivity
under
mild
conditions.
However,
the
traditional
batch
reaction
suffers
from
low
enzyme
stability,
cofactor
recycling,
and
poor
reusability.
Here,
we
present
continuous-flow
using
coimmobilized
dual
enzymes
synthesis
of
γ-/δ-lactones,
which
are
widely
used
in
fragrances
flavors.
Typically,
carbonyl
reductase
mutant
SmCRM5
Serratia
marcescens,
was
by
covalent
binding
BmGDH,
glucose
dehydrogenase
capable
recovering
recycling
NADPH.
After
immobilization,
BmGDH
exhibited
8.9-/8.7-fold
increase
catalytic
efficiency
(kcat/Km)
57-/15-fold
half-life
at
30
°C,
respectively.
We
demonstrated
that
continuous
flow
reactor
showed
higher
rate
space-time
yield
(1586
g·L–1
d–1)
than
free
immobilized
production
(R)-δ-decalactone.
This
can
run
continuously
more
650
h
99%
ee
80%
conversion,
total
volume
exceeds
1500
volumes.
The
robustness
this
system
provides
green
efficient
value-added
chemicals.
