Piperazine-Based Mitochondria-Immobilized pH Fluorescent Probe for Imaging Endogenous ONOO^– and Real-Time Tracking of Mitophagy

ACS Applied Bio Materials, Год журнала: 2022, Номер 5(6), С. 2777 - 2785

Опубликована: Май 16, 2022

ONOO– is mainly produced in mitochondria, and dysfunctional damaged mitochondria are degraded lysosomes through autophagy, so it important to synthesize a single probe for dual detection of mitophagy. Unfortunately, mitochondria-immobilized fluorescent probes mitophagy have not yet been developed. Hence, we first reported piperazine-based red-emitting (PMR), which only can detect but also could be used image cellular by the pH variations because protonation piperazine moiety. PMR was designed prepared introducing ring as response group, lipophilic cation targeting moiety, benzyl chloride immobilizing mitochondrial proteins thiol groups. displayed an enhanced fluorescence at 640 nm acidification. Using these advantages PMR, successfully visualizing process induced rapamycin or starvation, chloroquine inhibit rapamycin-induced prevent fusion autophagosomes lysosomes. showed good sensitivity with limit 23 nM ONOO–, applied imaging exogenous/endogenous ONOO–. Combining above design, may study detailed function ONOO–-associated physiological pathological processes.

Язык: Английский

---

An ultra-sensitive and highly selective fluorescent probe for nickel ions and its environmental and biological applications

Sensors and Actuators B Chemical, Год журнала: 2022, Номер 369, С. 132300 - 132300

Опубликована: Июнь 30, 2022

Язык: Английский

---

A lysosome-targeting ratiometric fluorescent probe used to detect Nitroxyl (HNO) in a Parkinson's disease model

Sensors and Actuators B Chemical, Год журнала: 2023, Номер 398, С. 134455 - 134455

Опубликована: Авг. 15, 2023

Язык: Английский

---

Selective and visual detection of triphosgene by a red turn-on fluorescent probe

Sensors and Actuators B Chemical, Год журнала: 2022, Номер 371, С. 132557 - 132557

Опубликована: Авг. 28, 2022

Язык: Английский

---

Hyperbranched polysiloxane-based probe with enhanced lipophilicity for visualizing ONOO− fluctuations in endoplasmic reticulum

Analytica Chimica Acta, Год журнала: 2023, Номер 1249, С. 340939 - 340939

Опубликована: Фев. 4, 2023

Язык: Английский

---