Inertial microfluidics: current status, challenges, and future opportunities

Lab on a Chip, Год журнала: 2022, Номер 22(24), С. 4792 - 4804

Опубликована: Янв. 1, 2022

We present the current status, challenges, and future opportunities of inertial microfluidics.

Язык: Английский

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Enhancing single-cell encapsulation in droplet microfluidics with fine-tunable on-chip sample enrichment

Microsystems & Nanoengineering, Год журнала: 2024, Номер 10(1)

Опубликована: Янв. 2, 2024

Abstract Single-cell encapsulation in droplet microfluidics is commonly hindered by the tradeoff between cell suspension density and on-chip focusing performance. In this study, we introduce a novel microfluidic chip to overcome challenge. The comprises double spiral unit, flow resistance-based sample enrichment module with fine-tunable outlets, crossflow generation unit. Utilizing low-density cell/bead (2 × 10 6 objects/mL), cells/beads are focused into near-equidistant linear arrangement within microchannel. excess water phase diverted while remain sequentially encapsulated individual droplets. Focusing performance was assessed through numerical simulations experiments at three rates (40, 60, 80 μL/min), demonstrating successful 40 μL/min for beads cells, respectively. addition, both simulation experimental results revealed that resistance adjustable punching different allowing over 50% of aqueous be removed. YOLOv8n-based detection algorithms realized counting droplets, statistically single-cell bead 72.2% 79.2%, All indicate approach can further developed employed as critical component water-in-oil

Язык: Английский

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Sheathless inertial particle focusing methods within microfluidic devices: a review

Frontiers in Bioengineering and Biotechnology, Год журнала: 2024, Номер 11

Опубликована: Янв. 8, 2024

The ability to manipulate and focus particles within microscale fluidic environments is crucial advancing biological, chemical, medical research. Precise high-throughput particle focusing an essential prerequisite for various applications, including cell counting, biomolecular detection, sample sorting, enhancement of biosensor functionalities. Active sheath-assisted techniques offer accuracy but necessitate the introduction external energy fields or additional sheath flows. In contrast, passive methods exploit inherent fluid dynamics in achieving without actuation. This review analyzes latest developments strategies sheathless inertial focusing, emphasizing elasto-inertial microfluidic from channel structure classifications. These methodologies will serve as pivotal benchmarks broader application technologies biological manipulation. Then, prospects future development are also predicted. paper assist understanding design devices.

Язык: Английский

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Label-Free and Rapid Microfluidic Design Rules for Circulating Tumor Cell Enrichment and Isolation: A Review and Simulation Analysis

ACS Omega, Год журнала: 2025, Номер 10(7), С. 6306 - 6322

Опубликована: Фев. 11, 2025

Enriching and isolating circulating tumor cells (CTCs) have attracted significant interest due to their important role in early cancer diagnosis prognosis, allowing for minimally invasive approaches providing vital information about metastasis at the cellular level. This review comprehensively summarizes recent developments microfluidic devices CTC enrichment isolation. The advantages limitations of several are discussed, design specifications highlighted. We also developed a set methodologies rules label-free microfluidics such as spiral, deterministic lateral displacement (DLD) dielectrophoresis (DEP) allow researchers develop systematically effectively, promoting rapid research on design, fabrication, experimentation.

Язык: Английский

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Effects of obstacles on inertial focusing and separation in sinusoidal channels: An experimental and numerical study

Chemical Engineering Science, Год журнала: 2023, Номер 276, С. 118826 - 118826

Опубликована: Май 4, 2023

Inertial microfluidics manipulates and separates microparticles based on the finite inertia of fluid at high flow speed. In inertial microfluidics, modifying geometry by embedding periodic micro-obstacles into curvilinear channels is emerging as a promising strategy to improve focusing separation. This work systematically investigated influence developed high-resolution microfluidic device for particle cell First, we numerical modelling simulate migration trajectories particles. Then, studied effects various obstacles in sinusoidal channels. The concave were more effective tuning separation than convex obstacles. Furthermore, square obstacle channel could offer highest resolution. Finally, channel, applied it high-efficiency polystyrene beads U87MG cancer cells from blood.

Язык: Английский

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Spiral Large-Dimension Microfluidic Channel for Flow-Rate- and Particle-Size-Insensitive Focusing by the Stabilization and Acceleration of Secondary Flow

Analytical Chemistry, Год журнала: 2024, Номер 96(4), С. 1750 - 1758

Опубликована: Янв. 12, 2024

Inertial microfluidics has demonstrated its ability to focus particles in a passive and straightforward manner. However, achieving flow-rate- particle-size-insensitive focusing large-dimension channels with simple design remains challenging. In this study, we developed spiral microfluidic channel achieve inertial focusing. By designing unique "big buffering area" "small the microchannel, observed stabilization acceleration of secondary flow. Our optimized allowed for efficient (>99.9%) 15 μm within wide range flow rates (0.5-4.5 mL/min) during long operation duration (0-60 min). Additionally, achieved effective (>95%) different-sized (7, 10, 15, 30 μm) three types tumor cells (K562, HeLa, MCF-7) near inner wall 1 mm outlet when applying different (1-3 mL/min). Finally, successful 3D cell was an device, positioned at distance 50 from wall. strategy stabilizing accelerating Dean-like through configuration proved be highly that is insensitive rate particle size, particularly channels. Consequently, it shows great potential use hand-operated tools cytometry.

Язык: Английский

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High-Throughput Blood Plasma Extraction in a Dimension-Confined Double-Spiral Channel

Analytical Chemistry, Год журнала: 2023, Номер 95(45), С. 16649 - 16658

Опубликована: Ноя. 2, 2023

Microfluidic technologies enabling the control of secondary flow are essential for successful separation blood cells, a process that is beneficial wide range medical research and clinical diagnostics. Herein, we introduce dimension-confined microfluidic device featuring double-spiral channel designed to regulate flows, thereby high-throughput isolation plasma extraction. By integrating sequence micro-obstacles within microchannels, stable enhanced Dean-like across each loop can be generated. This setup consequently prompts particles varying diameters (3, 7, 10, 15 μm) form different focusing states. Crucially, this system capable effectively separating cells sizes with cell throughput (2.63-3.36) × 108 cells/min. The concentration in outlet 2 increased 3-fold, from 1.46 4.37 108, while number including platelets, exported outlets 1 3 decreased by factor 608. engineering approach manipulating extraction points simplicity fabrication, ease operation, insensitivity size, high throughput, efficiency, which has potential utility propelling development miniaturized diagnostic devices field biomedical science.

Язык: Английский

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Multi-Vortex Regulation in a Simple Semicircular Microchannel with Ordered Micro-Obstacles for High-Throughput Buffer Exchange

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Янв. 20, 2025

Microfluidics is an emerging technology for buffer exchange in bioprocessing applications. However, achieving with simplicity of operation and high throughput a straightforward channel design remains challenge. This study presents novel semicircular microchannel that allows the deterministic regulation helical Dean vortices through geometric confinement. By incorporating micro-obstacles into microchannels large dimensions (900 μm wide 100 high), we observe substantial enhancement secondary flows, leading to unique fluid distribution across range flow rates. enables particle separation efficiency (>96.27%) coupled low fluorescein purity (<4.46%) at 3 × 106 particles/min. The proposed methodology, characterized by ease production (simple dimensions), user-friendly (uniform rates both sheath sample inlets), efficient capabilities (typically mL min–1), demonstrates significant potential advancing microfluidic systems biological biomedical research.

Язык: Английский

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High-Throughput Microfluidics for Precise Separation and Focusing of Circulating Tumor Cells with Optimized Triangular Microchannel Design

Talanta, Год журнала: 2025, Номер unknown, С. 128022 - 128022

Опубликована: Март 1, 2025

Язык: Английский

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Flow-Rate-Insensitive Plasma Extraction by the Stabilization and Acceleration of Secondary Flow in the Ultralow Aspect Ratio Spiral Channel

Analytical Chemistry, Год журнала: 2023, Номер 95(49), С. 18278 - 18286

Опубликована: Ноя. 28, 2023

Although microfluidic devices have made remarkable strides in blood cell separation, there is still a need for further development and improvement this area. Herein, we present novel ultralow aspect ratio (H/W = 1:36) spiral channel device with ordered micro-obstacles sheathless flow-rate-insensitive separation. By introducing into the microchannels, reduced magnitude fluctuations secondary flow across different loops can be obtained through geometric confinement. As result, unique Dean-like effectively enhance separation efficiency of particles sizes ranging from 3 to 15 μm. Compared most existing devices, our system offers several advantages easy manufacturing, convenient operation, long-term stability, highly efficient performance (up 99.70% rejection efficiency, including platelets), importantly, insensitivity as well rates (allowing different-sized cells wide rate 1.00 2.50 mL/min). The characteristics, such ratio, sequential micro-obstacles, controlled flow, make promising solution practical plasma extraction biomedical research clinical applications.

Язык: Английский

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