Gallic
acid
(GA),
widely
used
in
food
and
medicine
production
industries.
Herein,
a
kind
of
newly
synthesized
cobalt-nitrogen
co-doped
carbon
dots
(Co,N-CDs)
with
dual
functional
properties
oxidase-like
activity
photoluminescence
property
could
oxidize
the
3,3',5,5'-tetramethylbenzidine
(TMB)
to
oxTMB
new
absorption
peak
at
653
nm.
In
addition,
it
was
found
that
performed
fluorescence-quenching
effect
on
Co,N-CDs
440
nm
attributed
FRET
(Fluorescence
resonance
energy
transfer).
Reductive
GA
reduce
TMB,
weaken
blue
color,
restore
fluorescence
Co,N-CDs.
Thus,
colorimetric
fluorometric
method
for
dual-mode
detection
established.
The
synthesis
dual-signal
probe
completed
single
environmental
precursor
just
one
step.
Simultaneously,
reduced
limits,
broadened
linear
range
improved
anti-interference
ability.
This
exhibited
satisfactory
application
prospect
actual
tea
beverages.
Biomacromolecules,
Год журнала:
2024,
Номер
25(9), С. 5873 - 5888
Опубликована: Авг. 23, 2024
Metal-organic
frameworks
and
hydrogen-organic
(MOFs
HOFs)
are
attractive
hosts
for
enzyme
immobilization,
but
they
limited
to
immobilizing
the
purified
enzymes,
making
industrial
upscaling
unattractive.
Herein,
aptamer-modified
dual
thermoresponsive
polymeric
micelles
with
switchable
self-assembly
core-shell
structure
constructed,
which
enable
selective
immobilization
of
trypsin
directly
from
complex
biological
systems
through
a
cascade
operation
separation
immobilization.
Their
steric
provides
large
amount
adsorption
sites
on
soluble
micellar
shell,
resulting
in
high
capacity
excellent
selectivity.
Meanwhile,
their
aptamer
affinity
ligand
cavity
maintain
native
conformations
offer
protective
effects
even
harsh
conditions.
The
maximum
was
determined
be
197
mg/g
at
60
min,
superior
those
MOFs
HOFs.
67.2
86.6%
its
original
activity
retained
immobilized
under
strong
alkaline
acidic
conditions,
respectively.
Abstract
Nanozymes‐involving
colorimetry
may
represent
an
effective
solution
to
tetracycline
detection
due
its
affordability
and
simplicity.
To
improve
the
performance
in
selectivity,
molecular
imprinting
technology
has
emerged
as
a
promising
approach
simulating
interaction
between
antibodies
receptors.
Herein,
Mn
2
V
O
7
,
form
of
nanozyme
with
oxidase‐mimicking
activity,
was
synthesized.
With
template,
cavities
unique
dimensions
were
constructed
through
self‐polymerization
dopamine
(DA)
on
.
In
@MIP
polydopamine
(PDA)
acts
molecularly
imprinted
polymer
(MIP)
are
compatible
dimension.
It
also
exhibits
activity.
introduction
tetracycline,
cavity
PDA
is
blocked,
which
leads
obvious
color
fading,
enables
precise
colorimetry.
The
range
linear
from
10
100
μM,
lower
limit
0.82
μM.
anticipated
that
combination
nanozymes‐involving
would
contribute
accurate
organic
pollutants
aquatic
environment.
Gallic
acid
(GA),
widely
used
in
food
and
medicine
production
industries.
Herein,
a
kind
of
newly
synthesized
cobalt-nitrogen
co-doped
carbon
dots
(Co,N-CDs)
with
dual
functional
properties
oxidase-like
activity
photoluminescence
property
could
oxidize
the
3,3',5,5'-tetramethylbenzidine
(TMB)
to
oxTMB
new
absorption
peak
at
653
nm.
In
addition,
it
was
found
that
performed
fluorescence-quenching
effect
on
Co,N-CDs
440
nm
attributed
FRET
(Fluorescence
resonance
energy
transfer).
Reductive
GA
reduce
TMB,
weaken
blue
color,
restore
fluorescence
Co,N-CDs.
Thus,
colorimetric
fluorometric
method
for
dual-mode
detection
established.
The
synthesis
dual-signal
probe
completed
single
environmental
precursor
just
one
step.
Simultaneously,
reduced
limits,
broadened
linear
range
improved
anti-interference
ability.
This
exhibited
satisfactory
application
prospect
actual
tea
beverages.
Gallic
acid
(GA),
widely
used
in
food
and
medicine
production
industries.
Herein,
a
kind
of
newly
synthesized
cobalt-nitrogen
co-doped
carbon
dots
(Co,N-CDs)
with
dual
functional
properties
oxidase-like
activity
photoluminescence
property
could
oxidize
the
3,3',5,5'-tetramethylbenzidine
(TMB)
to
oxTMB
new
absorption
peak
at
653
nm.
In
addition,
it
was
found
that
performed
fluorescence-quenching
effect
on
Co,N-CDs
440
nm
attributed
FRET
(Fluorescence
resonance
energy
transfer).
Reductive
GA
reduce
TMB,
weaken
blue
color,
restore
fluorescence
Co,N-CDs.
Thus,
colorimetric
fluorometric
method
for
dual-mode
detection
established.
The
synthesis
dual-signal
probe
completed
single
environmental
precursor
just
one
step.
Simultaneously,
reduced
limits,
broadened
linear
range
improved
anti-interference
ability.
This
exhibited
satisfactory
application
prospect
actual
tea
beverages.
