Abstract
Fluorescence
signal
“turn‐on”
lateral
flow
immunoassay
(FONLFA)
through
nanomaterial
labeled
quenching
fluorescent
has
shown
significant
potential
for
the
detection
of
small
molecules.
However,
immobilization
on
nitrocellulose
(NC)
membrane
commonly
requires
tedious
chemical
modification
and
only
a
few
combinations
fluorescence
donor
quencher
have
been
applied
in
FONLFA.
In
this
work,
bright
metal
nanoclusters
(Prot‐AuNCs)
were
prepared
self‐assembled
into
Prot‐AuNCs/antigen
aggregates
with
three
typical
molecule
antigens,
respectively.
The
can
be
readily
immobilized
surface
NC
membrane,
indicating
that
strip
fabrication
strategy
good
versatility.
Moreover,
we
evaluated
performances
FONLFA
platform
by
using
carbendazim
as
model
target
investigated
four
nanomaterials
colorimetric
nanoprobes
quenchers.
We
found
all
demonstrated
significantly
improved
naked
eye
sensitivity
(vLOD)
limits
(LODs)
quantitative
analysis.
Among
them,
combing
Fe‐polydopamine
nanoparticles
above
aggregates,
mode
achieved
200‐fold
vLOD
(0.05
ng
mL
−1
)
compared
conventional
AuNPs‐based
(AuNPs‐LFA)
(10
).
addition,
LOD
analysis
also
was
22‐fold
whole
test
process
completed
within
10
min.
With
advantages
efficient
fabrication,
extraordinary
sensitization,
biocompatibility,
our
is
expected
to
great
rapid
various
Analytical Chemistry,
Год журнала:
2024,
Номер
96(26), С. 10714 - 10723
Опубликована: Июнь 24, 2024
Excessive
intake
of
estrogen
poses
significant
health
risks
to
the
human
body;
hence,
there
is
a
necessity
develop
rapid
detection
methods
monitor
its
levels
addition.
Gold
nanoparticles
(AuNPs),
commonly
utilized
as
colorimetric
signal
labels,
find
extensive
application
in
lateral
flow
immunoassay
(LFIA).
However,
sensitivity
traditional
AuNPs-LFIA
typically
constrained
by
low
molar
extinction
coefficients
and
reliance
on
single
signal.
Herein,
this
work,
unique
spark-type
AuCuPt
nanoflowers
modified
with
tannic
acid
(AuCuPt@TA)
were
precisely
designed
reasonable
layer-by-layer
element
composition
green
modification.
The
obtained
displays
robust
broadband
absorption
spanning
visible
near-infrared
spectrum,
showcasing
notable
coefficient
2.38
×
10
Materials Today Bio,
Год журнала:
2024,
Номер
28, С. 101188 - 101188
Опубликована: Авг. 7, 2024
Paper
based
point-of-care
(PoC)
detection
platforms
applying
lateral
flow
assays
(LFAs)
have
gained
paramount
approval
in
the
diagnostic
domain
as
well
environmental
applications
owing
to
their
ease
of
utility,
low
cost,
and
rapid
signal
readout.
It
has
centralized
aspect
self-evaluation
exhibiting
promising
potential
last
global
pandemic
era
Covid-19
implementing
management
public
health
remote
areas.
In
this
perspective,
present
review
is
focused
towards
landscaping
current
framework
LFAs
along
with
integration
components
characteristics
for
improving
assay
by
pushing
limits.
The
highlights
synergistic
aspects
designing,
sample
enrichment
strategies,
novel
nanomaterials-based
transducers,
high-end
analytical
techniques
that
contribute
significantly
sensitivity
specificity
enhancement.
Various
recent
studies
are
discussed
supporting
innovations
LFA
systems
focus
upon
accuracy
reliability
PoC
testing.
also
provides
a
comprehensive
overview
all
possible
difficulties
commercialization
subjecting
its
applicability
pathogen
surveillance,
water
food
testing,
disease
diagnostics,
agriculture
issues.
Biosensors,
Год журнала:
2025,
Номер
15(2), С. 68 - 68
Опубликована: Янв. 23, 2025
Multiplex
lateral
flow
assays
are
one
of
the
greatest
advancements
in
world
rapid
diagnostics,
achieving
performance
several
tests
one.
These
meet
basic
requirements
increasing
ease
use,
low
detection
limit,
and
high
specificity,
as
they
combine
use
novel
strategies,
such
exploitation
multiple
labels,
a
variety
amplification
methods.
have
proven
their
usefulness
many
different
areas,
including
clinical
food,
environmental
monitoring.
In
this
review
paper,
we
attempt
to
highlight
discuss
predominant
changes
multianalyte
LFAs,
related
principle,
development,
combination
with
other
Attention
is
paid
flexibility
challenges
associated
LFA
arrays,
strategies
improve
detectability,
sensitivity,
reliability
assays.
Therefore,
emphasizes
current
advances
field
underline
possible
impact
multiplex
LFAs
on
future
diagnostics
analytical
sciences.
Molecules,
Год журнала:
2024,
Номер
29(19), С. 4551 - 4551
Опубликована: Сен. 25, 2024
Immunoassays
have
been
widely
used
for
the
determination
of
various
analytes
in
fields
disease
diagnosis,
food
safety,
and
environmental
monitoring.
Dual-signal
immunoassays
are
now
advanced
integrated
detection
technologies
with
excellent
self-correction
self-validation
capabilities.
In
this
work,
we
summarize
recent
advances
development
optical
electrochemical
dual-signal
immunoassays,
including
colorimetric,
fluorescence,
surface-enhanced
Raman
spectroscopy
(SERS),
electrochemical,
electrochemiluminescence,
photoelectrochemical
methods.
This
review
particularly
emphasizes
working
principle
diverse
utilization
dual-functional
molecules
nanomaterials.
It
also
outlines
challenges
prospects
future
research
on
immunoassays.
