Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 16, 2025
Multiplexed
and
portable
detection
of
small
molecular
food
contaminants
in
complex
matrices
remains
a
challenge
analytical
chemistry.
Herein,
inspired
by
the
multiplexed
detection,
nucleic-acid-targeting
capability,
programmability
Thermus
thermophilus
argonaute
(TtAgo),
fingerprint
recognition
nanopore
technology,
we
proposed
TtAgo-assisted
encoding
strategy
to
detect
three
mycotoxins
simultaneously
(AFB1,
OTA,
ZEN).
In
this
method,
effective
signal
conversion
turns
into
DNA
reporters
that
were
observed
via
technology
.
general,
guide
DNAs
(gDNAs)
released
presence
mycotoxins,
followed
specific
cleavage
mediated
TtAgo
release
reporters.
The
translocation
produces
highly
distinguishable
information
due
favorable
resolution
nanopore.
Owing
high
nanopore,
method
displayed
low
LODs
(AFB1
67.18
fM,
OTA
13.32
ZEN
11.69
fM)
with
wide
linear
ranges.
Furthermore,
specificity
practical
application
also
verified
showed
satisfactory
results.
conclusion,
offers
novel
avenue
for
molecules.
As
global
pollution
continues
to
escalate,
timely
and
accurate
monitoring
is
essential
for
guiding
governance
safeguarding
public
health.
The
increasing
diversity
of
pollutants
across
environmental
matrices
poses
a
significant
challenge
instrumental
analysis
methods,
which
often
require
labor-intensive
time-consuming
sample
pretreatment.
Nanopore
technology,
an
emerging
single-molecule
technique,
presents
promising
solution
by
enabling
the
rapid
identification
multiple
targets
within
complex
mixtures
with
minimal
preparation.
A
wide
range
have
been
characterized
using
natural
biological
nanopores
or
artificial
solid-state
nanopores,
their
distinct
advantages
include
simple
preparation,
high
sensitivity,
onsite
analysis.
In
particular,
long-read
nanopore
sequencing
has
led
dramatic
improvements
in
analyses
microbial
communities,
allows
species-level
taxonomic
assignment
amplicon
sequencing,
simplifies
assembly
metagenomes.
this
Perspective,
we
review
latest
advancements
analyzing
chemical
through
sensing
techniques.
We
also
explore
challenges
that
remain
rapidly
evolving
field
provide
outlook
on
potential
transform
monitoring,
risk
assessment,
health
protection.
