Improving Three-Photon Fluorescence of Near-Infrared Quantum Dots for Deep Brain Imaging by Suppressing Biexciton Decay
Nano Letters,
Год журнала:
2024,
Номер
24(22), С. 6706 - 6713
Опубликована: Май 22, 2024
Three-photon
fluorescence
microscopy
(3PFM)
is
a
promising
brain
research
tool
with
submicrometer
spatial
resolution
and
high
imaging
depth.
However,
only
limited
materials
have
been
developed
for
3PFM
owing
to
the
rigorous
requirement
of
three-photon
(3PF)
process.
Herein,
under
guidance
band
gap
engineering
strategy,
CdTe/CdSe/ZnS
quantum
dots
(QDs)
emitting
in
near-infrared
window
are
designed
constructing
3PF
probes.
The
formation
type
II
structure
significantly
increased
absorption
cross
section
QDs
caused
delocalization
electron–hole
wave
functions.
time-resolved
transient
spectroscopy
confirmed
that
decay
biexcitons
was
suppressed
due
appropriate
alignment,
which
further
enhanced
efficiency
QDs.
By
utilizing
QD-based
probes,
high-resolution
cerebral
vasculature
realized
excited
by
1600
nm
femtosecond
laser,
indicating
possibility
deep
these
Язык: Английский
Redox Differences Between Neurons and Astrocytes In Vivo in Ischemic Brain Tissues of Rodents
Antioxidants and Redox Signaling,
Год журнала:
2025,
Номер
unknown
Опубликована: Май 19, 2025
Aims:
Reactive
oxygen
species
(ROS)
are
considered
to
play
a
key
damaging
role
in
brain
during
the
development
of
ischemic
stroke.
To
clarify
how
different
ROS
contribute
pathogenesis,
innovative
approaches
for
real-time
vivo
detection
redox
parameters
necessary.
Results:
Using
highly
sensitive
genetically
encoded
biosensor
HyPer7
and
fiber-optic
neurointerface
technology,
we
demonstrated
that
level
hydrogen
peroxide
(H2O2)
slowly
increases
neurons
astrocytes
area
rat
after
middle
cerebral
artery
occlusion
next
40
h;
notably,
is
somewhat
higher.
Raman
microspectroscopy
awake
mice
also
revealed
differences
between
mitochondria
acute
ischemia
caused
by
photothrombosis.
Astrocytes
overloading
electron
transport
chain
(ETC)
with
electrons
1
h
ischemia,
whereas
do
not
demonstrate
changes
amount
reduced
carries.
Innovation
Conclusion:
The
combination
novel
allows
detail
events
spatiotemporal
resolution.
We
difference
damaged
areas
vivo.
An
elevated
loading
astrocytic
ETC
phase
provides
basis
increased
generation
superoxide
anion
radical
(O2•-)
its
following
conversion
other
reactive
species.
However,
observed
H2O2
concentrations
at
later
pathogenesis
stages.
During
this
period,
did
an
electrons,
therefore,
may
be
phenomenon
secondary
events.
Antioxid.
Redox
Signal.
00,
000-000.
Язык: Английский
Ultrafast supercontinuum sculpting for two-photon spectroscopy and microscopy of ratiometric fluorescent indicators
Applied Physics Letters,
Год журнала:
2024,
Номер
124(24)
Опубликована: Июнь 10, 2024
We
present
a
compact
laser
system
for
quantitative
two-photon
excitation
spectra
measurements
and
ratiometric
imaging
of
fluorescent
protein
indicators.
The
fundamental
the
is
short
segment
photonic
crystal
fiber
(PCF),
which
supports
nonlinear
transformation
low-power
ultrashort
pulses
by
preserving
temporal
coherence,
this
generates
an
ultrafast
almost
octave-spanning
supercontinuum
(SC).
Accurate
sculpting
SC
its
amplitude
phase
modulation
provides
implementation
spectroscopic
microscopic
modalities.
one
was
exhibited
action
cross
section
measuring
genetically
encoded
sensing
proteins
vital
biochemical
parameters:
acidity
(SypHer3s),
concentration
hydrogen
peroxide
(HyPer3
HyPer7),
redox
status
NADH
glutathione
(RexYFP
Grx1-roGFP2),
hypohalous
acids
their
derivatives
(Hypocrates).
For
microscopy,
we
investigated
optimized
intensity
pump
pulse
profiles
under
high
numerical
objective
dispersion
scan
technique.
conducted
real-time
monitoring
dynamics
in
HeLa
cells
with
subcellular
spatial
resolution
means
Hyper7
sensors.
presented
hybrid
ideal
optical
toolbox
order
to
develop
sensors,
can
be
visualized
vivo
using
microscopy.
Язык: Английский
Long-term tracing of individual human neural cells using multiphoton microscopy and photoconvertible polymer capsules
Journal of The Royal Society Interface,
Год журнала:
2024,
Номер
21(219)
Опубликована: Окт. 1, 2024
The
study
of
human
neural
cells,
their
behaviour
and
migration
are
important
areas
research
in
the
biomedical
field,
particularly
for
potential
therapeutic
applications.
safety
using
cells
therapy
is
still
a
concern
due
to
lack
information
on
long-term
changes
that
may
occur.
While
current
methods
cell
tracing
explore
gene
manipulations,
we
elaborate
approaches
marking
with
no
genetic
interference.
In
this
study,
present
novel
method
labelling
tracking
cell-impregnatable
photoconvertible
polyelectrolyte
microcapsules.
These
capsules
demonstrated
low
cytotoxicity
effect
differentiation
ability
maintained
high
level
fluorescent
signal
individual
over
7
days.
modified
rhodamine-
fluorescein-based
dyes
were
undergo
photoconversion
by
both
one-
two-photon
lasers
while
being
internalized
cells.
finding
gives
possibility
select
inside
multicellular
structures
like
spheroids
tissues
alternate
appearance.
Thus,
can
track
paths
complex
systems.
This
new
offers
promising
alternative
studying
cells’
systems
such
as
three-dimensional
cellular
populations.
Язык: Английский