Ether Rhodamines with Enhanced Hydrophilicity, Fluorogenicity, and Brightness for Super-Resolution Imaging

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Янв. 2, 2025

ABSTRACT Rhodamine dyes are widely used fluorophores in super-resolution fluorescence imaging due to their exceptional optical properties and “aggregation-disaggregation” induced fluorogenic activation. However, excessive lipophilicity often reduces brightness aqueous environments causes off-target staining, limiting effectiveness high-resolution imaging. To address these challenges, we introduce an ether-decorated N-terminal modification strategy for rhodamine silicon-rhodamine (Si-rhodamine), replacing conventional N-alkyl groups. The ether chains enhance water solubility, decrease aggregate size, improve fluorogenicity across a wide concentration range. Their flexible, hydrophilic structure forms protective shield around the xanthene core, minimizing dye-water interactions reducing quenching. Additionally, inductive effect of decreases electron-donating strength amino groups, suppressing quenching caused by twisted intramolecular charge transfer (TICT). These modifications collectively increase quantum yields ER ESiR from 0.35 0.19 (for tetraethyl-substituted analogs) 0.70 0.41, respectively. Probes derived exhibit outstanding fluorogenicity, enhanced signal-to-noise ratios, improved resolution complex environments, demonstrating superior performance advanced techniques such as structured illumination microscopy (SIM), stimulated emission depletion (STED) microscopy, single-molecule localization (SMLM). This work introduces innovative fluorophore design, offering significant advancements applications.

Язык: Английский

Designing malachite green derivatives to optimize fluorogen-activating protein pairs for rapid PAINT super-resolution imaging in living cells

Chinese Chemical Letters, Год журнала: 2025, Номер unknown, С. 110980 - 110980

Опубликована: Фев. 1, 2025

Язык: Английский