Plasmonic nanosized molecularly imprinted POLYMER (nanomIP) as innovative optical lateral flow immunoassay probes

Sensors and Actuators B Chemical, Год журнала: 2025, Номер unknown, С. 137249 - 137249

Опубликована: Янв. 1, 2025

Язык: Английский

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Hapten prediction, monoclonal antibody preparation, and development of an immunochromatographic assay for the detection of fenamiphos

Journal of Hazardous Materials, Год журнала: 2025, Номер 487, С. 137168 - 137168

Опубликована: Янв. 8, 2025

Язык: Английский

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Bidirectional Drive Reverse-Phase Enhanced Fluorescence Lateral Flow Immunoassay with Spectral Overlap and Quantitative Balance for the Analysis of Deoxynivalenol

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Фев. 9, 2025

The capacity of the reverse-phase enhanced fluorescence (turn-on mode) to sensitively alter intensity from "zero" "one" has drawn increasing interest and investigation in competitive lateral flow immunoassay (LFIA). During this process, three important considerations must be made: designing an effective quenching agent, choosing a suitable donor, building mode. In work, we employed Au@MnO2 nanoparticles as highly quenchers AuNCs donors achieve overlap absorption emission spectra. Furthermore, have effectively created immunological network-based LFIA balance quantitative relationship for quick accurate detection deoxynivalenol (DON) toxin (Au@MnO2-GAB-CFLFIA). Notably, our proposed fluorescent turn-on mode demonstrated 11.65-fold sensitivity enhancement (0.0509 ng/mL) compared colorimetric (0.593 ng/mL). network shows 5.08-fold non-network (0.259 Moreover, satisfactory recoveries 93.33–109.02% are obtained real samples (maize, wheat, oat). DON detection, spectral offer more novel efficient strategies LFIA.

Язык: Английский

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Quantitative immunochromatographic assay for rapid detection of rosemary herb extract in ham sausage and dairy products

Food Chemistry, Год журнала: 2024, Номер 465, С. 142073 - 142073

Опубликована: Ноя. 15, 2024

Язык: Английский

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Ferrous Tungstate Nanomaterials with Excellent Enzyme-Mimicking Activity to Enhance Lateral Flow Immunoassay Sensitivity

Analytical Chemistry, Год журнала: 2025, Номер 97(5), С. 2714 - 2723

Опубликована: Янв. 30, 2025

Lateral flow immunochromatography (LFIA) with gold nanoparticles (AuNPs) is widely used in the biomedical field as a rapid and simple vitro detection technique. However, conventional AuNP-LFIA has limitations sensitivity range. In this study, nonprecious metal iron-based bimetallic FeWO4 nanomaterials convenient excellent enzyme-mimetic catalytic activities were synthesized by one-pot hydrothermal method. Here, combined C-reactive protein (CRP) antibodies to form novel signal-enhancing probe for analysis of CRP. The further achieves significant signal amplification catalyzing oxidation reaction 3-amino-9-ethylcarbazole LFIA assay, thereby improving accuracy assay. application FeWO4-based improved limit CRP 19.38 ng/mL after amplification, which approximately 30-fold lower than that addition, method demonstrated good stability reproducibility, providing promising prospective strategy early diagnosis inflammatory diseases.

Язык: Английский

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Enhancing meat safety and quality: Innovations in protein-based sensing technologies for contaminant detection

Food Control, Год журнала: 2025, Номер unknown, С. 111208 - 111208

Опубликована: Фев. 1, 2025

Язык: Английский

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Nanobody-Based Lateral Flow Assay for Rapid Zika Virus Detection

ACS Synthetic Biology, Год журнала: 2025, Номер unknown

Опубликована: Март 7, 2025

Zika virus infections remain severely underdiagnosed due to their initial mild clinical symptoms. However, recent outbreaks have revealed neurological complications in adults and severe deformities newborns, emphasizing the critical need for accurate diagnosis. Lateral flow assays (LFAs) provide a rapid, cost-effective, user-friendly method antigen testing at point-of-care, bedside, or home settings. LFAs utilizing nanobodies multiple benefits over traditional antibody-based techniques, as are much smaller, more stable, simpler manufacture. We introduce nanobody-based LFA rapid identification of antigens. Starting from two previously reported recognizing nonstructural protein 1 (NS1), we evaluate periplasmic cytosolic nanobody expression test different purification tags immobilization strategies. quantify binding kinetics validate mutually noncompetitive binding. Avidity effects boost capture tetrameric target by 3 orders magnitude point general strategy higher sensitivity sensing. The detects NS1 with limit detection ranging 25 ng/mL buffer urine. This nanobody-LFA has potential facilitate on-site self-diagnosis, improve our understanding infection prevalence, support public health initiatives regions affected outbreaks.

Язык: Английский

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Comparative Analysis of Immunization Strategies for Broad-Specificity Antibody Preparation and Immunoassay Development for Sulfonamides Detection in Chicken and Pork Samples

Food Bioscience, Год журнала: 2025, Номер unknown, С. 106350 - 106350

Опубликована: Март 1, 2025

Язык: Английский

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Black palladium nanoparticle-based lateral flow immunoassay for cardiovascular disease diagnosis

RSC Advances, Год журнала: 2025, Номер 15(20), С. 15490 - 15496

Опубликована: Янв. 1, 2025

The PdNP-based LFIA for the detection of Lp-PLA2 address sensitivity limitations colorimetric LFIA, due to excellent visual discriminability and target recognition ability.

Язык: Английский

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A colorimetric and SERS-based LFIA for sensitive and simultaneous detection of three stroke biomarkers: An ultra-fast and sensitive point-of-care testing platform

Talanta, Год журнала: 2024, Номер 283, С. 127166 - 127166

Опубликована: Ноя. 6, 2024

Язык: Английский

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